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Literature summary for 3.5.1.4 extracted from
- Overproduction and rapid purification of the amidase of Streptococcus pneumoniae (1987), Arch. Microbiol., 149, 52-56.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
strain RB791, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
and strain HB101 | - |
| Escherichia coli RB791 | - |
and strain HB101 | - |
| Streptococcus pneumoniae | - |
wild-type strain R6 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified in a single-step procedure using a choline-Sepharose 6B column | Streptococcus pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
- |
Streptococcus pneumoniae |
| 600 | - |
strain RB971 harboring pGL100 with lactose | Escherichia coli |
| 1600 | - |
strain HB101 harboring pGL80 | Escherichia coli |
| 1700 | - |
strain HB101 harboring pGL81 | Escherichia coli |
| 10000 | - |
strain RB971 harboring pGL100 without lactose | Escherichia coli |
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