Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Pseudomonas aeruginosa | |
iodoacetamide | no effect of 2-chloropropionamide | Pseudomonas putida | |
N-ethylmaleimide | inhibition at 10 mM, C-amidase | Pseudomonas putida | |
p-hydroxymercuribenzoate | - |
Pseudomonas aeruginosa | |
p-hydroxymercuribenzoate | inhibition at 10 mM, inhibits the C-amidase | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80 | - |
2-Chloropropionamide | A-amidase | Pseudomonas putida | |
100 | - |
acetamide | A-amidase | Pseudomonas putida | |
206 | - |
2-Chloropropionamide | strain PPW3, C-amidase | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
strain PPW3, C-amidase, determined by gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloropropionamide + H2O | Pseudomonas putida | - |
2-chloropropionate + NH3 | - |
? | |
2-chloropropionamide + H2O | Pseudomonas putida PP3 | - |
2-chloropropionate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
PAU3 and PAU5 | - |
Pseudomonas putida | - |
and strain PPW3 | - |
Pseudomonas putida PP3 | - |
and strain PPW3 | - |
Purification (Comment) | Organism |
---|---|
strain PPW3, C-amidase, ammonium sulfate fractionation, gel filtration, DEAE-Sephadex, preparative PAGE, purification factor 40 | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00003 | - |
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3 | Pseudomonas putida |
0.00024 | - |
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3 | Pseudomonas putida |
0.00027 | - |
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PP3 | Pseudomonas putida |
0.0017 | - |
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU3 | Pseudomonas aeruginosa |
0.019 | - |
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3 | Pseudomonas putida |
0.023 | - |
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3 | Pseudomonas putida |
0.031 | - |
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5 | Pseudomonas putida |
0.039 | - |
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5 | Pseudomonas putida |
0.078 | - |
A-amidase, substrate was 2-chloropropionamide | Pseudomonas putida |
0.12 | - |
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PPW3 | Pseudomonas putida |
0.14 | - |
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5 | Pseudomonas putida |
0.25 | - |
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PAU5 | Pseudomonas putida |
0.914 | - |
strain PPW, activity of C-amidase after preparative PAGE, final yield 17% of original activity | Pseudomonas putida |
1.89 | - |
strain PPW3, C-amidase, substrate 2-chloropropionamide | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloropropionamide + H2O | - |
Pseudomonas putida | 2-chloropropionate + NH3 | - |
? | |
2-chloropropionamide + H2O | - |
Pseudomonas putida PP3 | 2-chloropropionate + NH3 | - |
? | |
acetamide + H2O | - |
Pseudomonas putida | acetate + NH3 | - |
? | |
acetamide + H2O | - |
Pseudomonas putida PP3 | acetate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | strain PPW3, C-amidase, subunit molecular mass of 29000 Da, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
A-amidase | specificity towards aliphatic amides | Pseudomonas aeruginosa |
C-amidase | specificity towards chlorinated aliphatic amides | Pseudomonas putida |