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Literature summary for 3.5.1.4 extracted from

  • Hirrlinger, B.; Stolz, A.; Knackmuss, H.J.
    Purification and properties of an amidase from Rhodococcus erythropolis MP50 which enantioselectively hydrolyzes 2-arylpropionamides (1996), J. Bacteriol., 178, 3501-3507.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.4

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0% of origin activity Rhodococcus erythropolis
Cu2+ 0% of origin activity Rhodococcus erythropolis
dithiothreitol 97% of origin activity Rhodococcus erythropolis
iodoacetate 81% of origin activity Rhodococcus erythropolis
p-hydroxymercuribenzoate 53% of origin activity Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.067
-
 ketoprofen amide
-
 Rhodococcus erythropolis
0.069
-
 phenylacetamide
-
 Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 8 * 61000, SDS-PAGE Rhodococcus erythropolis
480000
-
 gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ketoprofen amide Rhodococcus erythropolis
-
 2-(3-benzoylphenyl)propionic acid + NH3
-
 ?
ketoprofen amide Rhodococcus erythropolis MP50
-
 2-(3-benzoylphenyl)propionic acid + NH3
-
 ?
phenylacetamide Rhodococcus erythropolis
-
 phenylacetic acid + NH3
-
 ?
phenylacetamide Rhodococcus erythropolis MP50
-
 phenylacetic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
-
-
Rhodococcus erythropolis MP50
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion-exchange chromatography, phenyl-Superose column, desalted with a Hitrap desalting column Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
 substrate urea Rhodococcus erythropolis
0.67
-
 activity in cell extract, substrate 1 mM phenylacetamide Rhodococcus erythropolis
0.81
-
 activity in cell extract, substrate 1 mM ketoprofen amide Rhodococcus erythropolis
1.3
-
 activity in cell extract, substrate 1 mM naproxen amide Rhodococcus erythropolis
4.1
-
 activity after purification, substrate 5 mM phenylacetamide Rhodococcus erythropolis
4.83
-
 substrate methacrylamide Rhodococcus erythropolis
6.69
-
 substrate cyclohexanamide Rhodococcus erythropolis
16.39
-
 substrate hexanamide Rhodococcus erythropolis

Storage Stability

Storage Stability Organism
room temperature, half-life about 2000 days with ammonium sulfate Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ketoprofen amide
-
 Rhodococcus erythropolis 2-(3-benzoylphenyl)propionic acid + NH3
-
 ?
ketoprofen amide
-
 Rhodococcus erythropolis MP50 2-(3-benzoylphenyl)propionic acid + NH3
-
 ?
phenylacetamide
-
 Rhodococcus erythropolis phenylacetic acid + NH3
-
 ?
phenylacetamide
-
 Rhodococcus erythropolis MP50 phenylacetic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 61000, SDS-PAGE Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Rhodococcus erythropolis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 65
-
 Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rhodococcus erythropolis

pH Range

pH Minimum pH Maximum Comment Organism
2 10
-
 Rhodococcus erythropolis