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Literature summary for 3.5.1.4 extracted from

  • Kobayashi, M.; Komeda, H.; Nagasawa, T.; Nishiyama, M.; Hoinouchi, Sueharu.; Beppu, T.; Yamada, H.; Shimizu.
    Amidase couple with low-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1 (1993), Eur. J. Biochem., 217, 327-336.
    View publication on PubMed
Search for more literature for 3.5.1.4

Application

Application Comment Organism
synthesis acrylamide production Rhodococcus rhodochrous

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 22% of origin activity Escherichia coli
Cu2+ 58% of origin activity Escherichia coli
Fe2+ 58% of origin activity Escherichia coli
Hg2+ 7% of the original activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 Benzamide
-
 Escherichia coli
0.48
-
 Propionamide
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54630
-
-
 Rhodococcus rhodochrous
55000
-
 2 * 55000 Escherichia coli
110000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-(+)-2-chloropropionamide + H2O Escherichia coli
-
 (+)-2-chloropropionic acid + NH3
-
 ?
(R)-(+)-2-chloropropionamide + H2O Escherichia coli JM109
-
 (+)-2-chloropropionic acid + NH3
-
 ?
(S)-(-)-2-chloropropionamide + H2O Escherichia coli
-
 (-)-2-chloropropionic acid + NH3
-
 ?
(S)-(-)-2-chloropropionamide + H2O Escherichia coli JM109
-
 (-)-2-chloropropionic acid + NH3
-
 ?
benzamide + H2O Escherichia coli
-
 benzoic acid + NH3
-
 ?
benzamide + H2O Escherichia coli JM109
-
 benzoic acid + NH3
-
 ?
RCONH2 + H2O Escherichia coli
-
 RCOOH + NH3
-
 ?
RCONH2 + H2O Rhodococcus rhodochrous
-
 RCOOH + NH3
-
 ?
RCONH2 + H2O Escherichia coli JM109
-
 RCOOH + NH3
-
 ?
RCONH2 + H2O Rhodococcus rhodochrous J1
-
 RCOOH + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 harboring pALJ30 and pALJ20
-
Escherichia coli JM109
-
 harboring pALJ30 and pALJ20
-
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous J1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
preparation of cell-free extracts by sonification, DEAE-Sephacel column chromatography, ammonium sulfate fraction, Mono-Q column chromatography, Superose 12 column chromatography, yield 30.4% Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00243
-
 harboring pALJ20, substrate cyclohexanecarboxamide with cobalt Escherichia coli
0.0135
-
 substrate cyclohexanecarboxamide with cobalt Rhodococcus rhodochrous
0.468
-
 harboring pALJ30, substrate cyclohexanecarboxamide with cobalt Escherichia coli
12.2
-
 substrate benzamide Escherichia coli
12.2
-
 transformant, substrate benzamide Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-(+)-2-chloropropionamide + H2O
-
 Escherichia coli (+)-2-chloropropionic acid + NH3
-
 ?
(R)-(+)-2-chloropropionamide + H2O
-
 Escherichia coli JM109 (+)-2-chloropropionic acid + NH3
-
 ?
(S)-(-)-2-chloropropionamide + H2O
-
 Escherichia coli (-)-2-chloropropionic acid + NH3
-
 ?
(S)-(-)-2-chloropropionamide + H2O
-
 Escherichia coli JM109 (-)-2-chloropropionic acid + NH3
-
 ?
benzamide + H2O
-
 Escherichia coli benzoic acid + NH3
-
 ?
benzamide + H2O
-
 Escherichia coli JM109 benzoic acid + NH3
-
 ?
propionamide + H2O
-
 Escherichia coli propionate + NH3
-
 ?
propionamide + H2O
-
 Escherichia coli JM109 propionate + NH3
-
 ?
RCONH2 + H2O
-
 Escherichia coli RCOOH + NH3
-
 ?
RCONH2 + H2O
-
 Rhodococcus rhodochrous RCOOH + NH3
-
 ?
RCONH2 + H2O
-
 Escherichia coli JM109 RCOOH + NH3
-
 ?
RCONH2 + H2O
-
 Rhodococcus rhodochrous J1 RCOOH + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 55000 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.7 10
-
 Escherichia coli