Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.4 extracted from

  • Soong, C.L.; Ogawa, J.; Shimizu, S.
    A novel amidase, half-amidase, for half-amide hydrolysis involved in the metabolism of cyclic imides (2000), Appl. Environ. Microbiol., 66, 1947-1952.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis
-
Blastobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ inactivates enzyme activity by 76% Blastobacter sp.
Cd2+ inactivates enzyme activity by 79% Blastobacter sp.
Cu2+ inactivates enzyme activity by 90% Blastobacter sp.
Hg2+ inactivates enzyme activity by 99% Blastobacter sp.
succinate
-
Blastobacter sp.
sulfhydyl reagents
-
Blastobacter sp.
Zn2+ inactivates enzyme activity by 88% Blastobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
glutaramic acid
-
Blastobacter sp.
6.2
-
succinamic acid
-
Blastobacter sp.
6.5
-
Benzamide
-
Blastobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE Blastobacter sp.
48000
-
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE Blastobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzamide Blastobacter sp.
-
benzoic acid + NH3
-
?
glutaramic acid + H2O Blastobacter sp.
-
glutaric acid + NH3
-
?
monoamidated dicarboxylate + H2O Blastobacter sp.
-
dicarboxylate + ammonia
-
?
succinamic acid + H2O Blastobacter sp.
-
succinic acid + NH3
-
?

Organism

Organism UniProt Comment Textmining
Blastobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cell extract purified by DEAE-Sephacel column, concentrated by ultrafiltration, phenyl-Sepharose column, Sephacryl S-200 column, Mono Q HR 5/5 and Mono Q PC 1.6/5 column Blastobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzamide
-
Blastobacter sp. benzoic acid + NH3
-
?
glutaramic acid + H2O
-
Blastobacter sp. glutaric acid + NH3
-
?
monoamidated dicarboxylate + H2O
-
Blastobacter sp. dicarboxylate + ammonia
-
?
succinamic acid + H2O
-
Blastobacter sp. succinic acid + NH3
-
?

Subunits

Subunits Comment Organism
monomer 1 * 48000, gel filtration and 1 * 46000, SDS-PAGE Blastobacter sp.

Synonyms

Synonyms Comment Organism
half-amidase for half-amide hydrolysis Blastobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Blastobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00217
-
Benzamide
-
Blastobacter sp.
0.0372
-
glutaramic acid
-
Blastobacter sp.
0.096
-
succinamic acid
-
Blastobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 various buffer systems, 80% of the activity retained at 30°C for 30 min Blastobacter sp.