Application | Comment | Organism |
---|---|---|
synthesis | - |
Blastobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | inactivates enzyme activity by 76% | Blastobacter sp. | |
Cd2+ | inactivates enzyme activity by 79% | Blastobacter sp. | |
Cu2+ | inactivates enzyme activity by 90% | Blastobacter sp. | |
Hg2+ | inactivates enzyme activity by 99% | Blastobacter sp. | |
succinate | - |
Blastobacter sp. | |
sulfhydyl reagents | - |
Blastobacter sp. | |
Zn2+ | inactivates enzyme activity by 88% | Blastobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
glutaramic acid | - |
Blastobacter sp. | |
6.2 | - |
succinamic acid | - |
Blastobacter sp. | |
6.5 | - |
Benzamide | - |
Blastobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE | Blastobacter sp. |
48000 | - |
1 * 48000, gel filtration and 1 * 46000, SDS-PAGE | Blastobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzamide | Blastobacter sp. | - |
benzoic acid + NH3 | - |
? | |
glutaramic acid + H2O | Blastobacter sp. | - |
glutaric acid + NH3 | - |
? | |
monoamidated dicarboxylate + H2O | Blastobacter sp. | - |
dicarboxylate + ammonia | - |
? | |
succinamic acid + H2O | Blastobacter sp. | - |
succinic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastobacter sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cell extract purified by DEAE-Sephacel column, concentrated by ultrafiltration, phenyl-Sepharose column, Sephacryl S-200 column, Mono Q HR 5/5 and Mono Q PC 1.6/5 column | Blastobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzamide | - |
Blastobacter sp. | benzoic acid + NH3 | - |
? | |
glutaramic acid + H2O | - |
Blastobacter sp. | glutaric acid + NH3 | - |
? | |
monoamidated dicarboxylate + H2O | - |
Blastobacter sp. | dicarboxylate + ammonia | - |
? | |
succinamic acid + H2O | - |
Blastobacter sp. | succinic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48000, gel filtration and 1 * 46000, SDS-PAGE | Blastobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
half-amidase | for half-amide hydrolysis | Blastobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Blastobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00217 | - |
Benzamide | - |
Blastobacter sp. | |
0.0372 | - |
glutaramic acid | - |
Blastobacter sp. | |
0.096 | - |
succinamic acid | - |
Blastobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | various buffer systems, 80% of the activity retained at 30°C for 30 min | Blastobacter sp. |