Application | Comment | Organism |
---|---|---|
synthesis | - |
Brevibacterium sp. |
synthesis | degradation of acrylamide | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aromatic amide | - |
Rhodococcus sp. | |
Co2+ | - |
Rhodococcus sp. | |
Cu2+ | - |
Rhodococcus sp. | |
Fe2+ | activity enhanced | Rhodococcus sp. | |
Hg2+ | - |
Rhodococcus sp. | |
iodoacetate | - |
Pseudomonas aeruginosa | |
iodoacetate | - |
Rhodococcus sp. | |
Mg2+ | partially inhibited | Rhodococcus sp. | |
Ni2+ | - |
Rhodococcus sp. | |
specific iron chelator | - |
Rhodococcus sp. | |
thiol blocking reagent | - |
Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
Acrylamide | - |
Rhodococcus sp. | |
2.6 | - |
Propionamide | - |
Rhodococcus sp. | |
2.7 | - |
acetamide | - |
Rhodococcus sp. | |
3.2 | - |
butyramide | - |
Rhodococcus sp. | |
3.5 | - |
Isobutyramide | - |
Rhodococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activity enhanced | Rhodococcus sp. | |
Cr2+ | activity enhanced | Rhodococcus sp. | |
Iron | 8 mol of iron per mol of enzyme, detection by inductively coupled plasma-atomic emission spectrometry | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, SDS-PAGE | Pseudomonas sp. |
35000 | - |
6 * 35000, SDS-PAGE | Pseudomonas aeruginosa |
43000 | - |
gel filtration | Pseudomonas sp. |
43000 | - |
4 * 43000 | Brevibacterium sp. |
44500 | - |
8 * 44500, SDS-PAGE | Rhodococcus sp. |
180000 | - |
- |
Brevibacterium sp. |
200000 | - |
gel filtration and sedimentation equilibrium analysis, sedimentation coeffient 10.6 S | Pseudomonas aeruginosa |
360000 | - |
PAGE, nondenaturing conditions | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylamide + H2O | Rhodococcus sp. | - |
acrylic acid + NH3 | - |
? | |
butyramide + H2O | Rhodococcus sp. | - |
n-butyrate + NH3 | - |
? | |
isobutyramide + H2O | Rhodococcus sp. | - |
isobutyrate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sp. | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Rhodococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitation, ammonium sulfate fractionation, DEAE-Sepharose chromatography, Mono Q column and gel filtration, purified approximately 138fold with a yield of 20% | Rhodococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.6 | - |
micromol of NH3 * min-1 * mg of protein-1 | Pseudomonas sp. |
274.1 | - |
micromol of NH3 * min-1 * mg of protein-1 | Brevibacterium sp. |
3500 | - |
micromol of NH3 * min-1 * mg of protein-1 | Rhodococcus sp. |
5000 | - |
substrate isobutyramide | Rhodococcus sp. |
5900 | - |
substrate acrylamide | Rhodococcus sp. |
7700 | - |
substrate butyramide | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylamide + H2O | - |
Rhodococcus sp. | acrylic acid + NH3 | - |
? | |
butyramide + H2O | - |
Rhodococcus sp. | n-butyrate + NH3 | - |
? | |
isobutyramide + H2O | - |
Rhodococcus sp. | isobutyrate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Pseudomonas sp. |
hexamer | 6 * 35000, SDS-PAGE | Pseudomonas aeruginosa |
octamer | 8 * 44500, SDS-PAGE | Rhodococcus sp. |
tetramer | 4 * 43000 | Brevibacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
at 40°C | Rhodococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Rhodococcus sp. |