Application | Comment | Organism |
---|---|---|
synthesis | acrylamide production | Pseudomonas chlororaphis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | preincubation for 30 min, remaining activity in presence of 1 mM DTT | Pseudomonas chlororaphis | |
Cd2+ | preincubated for 30 min | Pseudomonas chlororaphis | |
Cu2+ | preincubated for 30 min, remaining activity in presence of 1 mM DTT | Pseudomonas chlororaphis | |
DTNB | preincubated for 30 min, remaining activity in presence of 1 mM DTT | Pseudomonas chlororaphis | |
Fe2+ | preincubated for 30 min | Pseudomonas chlororaphis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Pseudomonas chlororaphis |
105000 | - |
- |
Pseudomonas chlororaphis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
propionamide + H2O | Pseudomonas chlororaphis | - |
propionic acid + NH3 | - |
? | |
propionamide + H2O | Pseudomonas chlororaphis B23 | - |
propionic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas chlororaphis | - |
- |
- |
Pseudomonas chlororaphis B23 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, 30 to 55%, and hydrophobic, anion-exchange, gel filtration, and ceramic hydroxyapatite chromatography steps, DTT required to maintain enzymatic activity, overall level of purification 114-fold, recovery rate 21% | Pseudomonas chlororaphis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.7 | - |
turnover propionamide to propionate and ammonia | Pseudomonas chlororaphis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propionamide + H2O | - |
Pseudomonas chlororaphis | propionic acid + NH3 | - |
? | |
propionamide + H2O | - |
Pseudomonas chlororaphis B23 | propionic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Pseudomonas chlororaphis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.5 | - |
Propionamide | - |
Pseudomonas chlororaphis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.4 | 11 | 100% activity at pH values between 5.9 and 9.9 | Pseudomonas chlororaphis |
7 | 8.6 | against the substrate propionamide | Pseudomonas chlororaphis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.9 | 9.9 | in various buffer | Pseudomonas chlororaphis |