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Literature summary for 3.5.1.36 extracted from
- The enzymatic synthesis of 5-hydroxy-N-methylpyroglutamic acid (1969), J. Biol. Chem., 244, 4677-4683.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by triethanolamine, 2-methylimidazole, diphosphate and Tricine buffer | Pseudomonas sp. | |
| Tris-HCl | - |
Pseudomonas sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38 | - |
alpha-ketoglutarate | - |
Pseudomonas sp. | |
| 46 | - |
methylamine | - |
Pseudomonas sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-ketoglutarate + methylamine | Pseudomonas sp. | - |
N-methyl-2-oxoglutaramate + H2O | - |
? | |
| alpha-ketoglutarate + methylamine | Pseudomonas sp. MA / M.A. / ATCC 23819 | - |
N-methyl-2-oxoglutaramate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
| Pseudomonas sp. MA / M.A. / ATCC 23819 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.98 | - |
after partial purification | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-ketoglutarate + methylamine | - |
Pseudomonas sp. | N-methyl-2-oxoglutaramate + H2O | - |
? | |
| alpha-ketoglutarate + methylamine | - |
Pseudomonas sp. MA / M.A. / ATCC 23819 | N-methyl-2-oxoglutaramate + H2O | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
after heated 12 min to 50°C the activity of enzyme is 12%. Untreated enzyme stable | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
enzymatic reaction exhibits a pH maximum of about 8 | Pseudomonas sp. |
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