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Literature summary for 3.5.1.36 extracted from

  • Hersh, L.B.
    5-Hydroxy-N-methylpyroglutamate synthase. Purification and mechanism of action (1970), J. Biol. Chem., 245, 3526-3535.
    View publication on PubMed
Search for more literature for 3.5.1.36

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxy-4-methoxy-benzophenone with 1 micromol 2-hydroxy-4-methoxy-benzophenone the activity is 0% after 10 min Pseudomonas sp.
DTNB with 20 micromol DTNB the activity is 5% after 10 min Pseudomonas sp.
iodoacetamide with 20 micromol iodoacetamide the activity is 20% after 10 min Pseudomonas sp.
iodoacetate with 20 micromol iodoacetate the activity is 33% after 10 min Pseudomonas sp.
N-Methylmaleimide with 20 micromol N-methylmaleimide the activity is 2% after 10 min Pseudomonas sp.
NEM with 20 micromol NEM the activity is 0% after 10 min Pseudomonas sp.
oxalacetate a competitive inhibitor Pseudomonas sp.
p-hydroxymercurisulfonate with 1 micromol p-hydroxymercurisulfonate the activity is 0% after 10 min Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 alpha-ketoglutaramate
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-ketoglutaramate + H2O Pseudomonas sp.
-
 alpha-ketoglutarate + NH3
-
 ?
alpha-ketoglutaramate + H2O Pseudomonas sp. MA / M.A. / ATCC 23819
-
 alpha-ketoglutarate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-

Purification (Commentary)

Purification (Comment) Organism
extraction, addition of streptomycin sulfate, adjust the pH value, centrifugation, ammonium sulfate precipitation, dialysis, purification with DEAE, Sephadex and hydroxyapatite column with dialysis and ultrafiltration steps Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
 after purification Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, 20 mM potassium phosphate buffer, pH 7.4, containing 5 mM alpha-ketoglutarate, six months. In the absence of alpha-ketoglutarate the enzyme slowly loses activity over a period of several weeks Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ketoglutaramate + H2O
-
 Pseudomonas sp. alpha-ketoglutarate + NH3
-
 ?
alpha-ketoglutaramate + H2O
-
 Pseudomonas sp. MA / M.A. / ATCC 23819 alpha-ketoglutarate + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0178
-
 alpha-ketoglutarate turnover number based on the molecular weight of 90000 Pseudomonas sp.