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Literature summary for 3.5.1.32 extracted from

  • Miyagawa, E.; Yano, J.; Hamakado, T.; Kido, Y.; Nishimoto, K.; Motoki, Y.
    Crystallization and properties of N-benzoylglycine amidohydrolase from Pseudomonas putida (1985), Agric. Biol. Chem., 49, 2881-2886.
No PubMed abstract available
Search for more literature for 3.5.1.32

Crystallization (Commentary)

Crystallization (Comment) Organism
tetrahydral crystals Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 80% inhibition at 1 mM Pseudomonas putida
Ag2+ complete inhibition at 1 mM Pseudomonas putida
Ba2+ slight inhibition at 1 mM Pseudomonas putida
Co2+ slight inhibition at 1 mM Pseudomonas putida
Cu2+ complete inhibition at 1 mM Pseudomonas putida
Fe2+ 80% inhibition at 1 mM Pseudomonas putida
Fe3+ 42% inhibition at 1 mM Pseudomonas putida
Hg2+ complete inhibition at 1 mM Pseudomonas putida
iodoacetic acid 60% inhibition at 1 mM Pseudomonas putida
Mn2+ 60% inhibition at 1 mM Pseudomonas putida
Ni2+ complete inhibition at 1 mM Pseudomonas putida
o-phenanthroline complete inhibition at 1 mM Pseudomonas putida
p-chloromercuribenzoic acid complete inhibition at 1 mM Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 4-chloro-N-benzoylglycine
-
 Pseudomonas putida
0.54
-
 4-acetyl-N-benzoylglycine
-
 Pseudomonas putida
0.54
-
 N-[(4-hydroxyphenyl)carbonyl]glycine
-
 Pseudomonas putida
0.71
-
 4-chloro-N-benzoyl-L-alanine
-
 Pseudomonas putida
0.72
-
 hippurate
-
 Pseudomonas putida
0.87
-
 N-benzoyl-L-alanine
-
 Pseudomonas putida
0.87
-
 N-benzoyl-L-aminobutyric acid
-
 Pseudomonas putida
0.95
-
 4-hydroxy-N-benzoyl-L-alanine
-
 Pseudomonas putida
1.1
-
 4-acetyl-N-benzoyl-L-alanine
-
 Pseudomonas putida
1.2
-
 4-nitro-N-benzoylglycine
-
 Pseudomonas putida
1.25
-
 4-nitro-N-benzoyl-L-alanine
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 4 * 42000, SDS-PAGE Pseudomonas putida
170000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
cell culture induction of enzyme synthesis by hippurate-containing media Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
426
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-acetyl-N-benzoyl-L-alanine + H2O low activity Pseudomonas putida 4-acetylbenzoic acid + L-alanine
-
 ?
4-acetyl-N-benzoylglycine + H2O
-
 Pseudomonas putida 4-acetylbenzoic acid + glycine
-
 ?
4-chloro-N-benzoyl-L-alanine + H2O
-
 Pseudomonas putida 4-chlorobenzoic acid + L-alanine
-
 ?
4-chloro-N-benzoylglycine + H2O
-
 Pseudomonas putida 4-chlorobenzoic acid + glycine
-
 ?
4-hydroxy-N-benzoyl-L-alanine low activity Pseudomonas putida 4-hydroxybenzoic acid + L-alanine
-
 ?
4-hydroxy-N-benzoylglycine + H2O
-
 Pseudomonas putida 4-hydroxybenzoic acid + glycine
-
 ?
4-hydroxy-N-benzoylglycine-L-histidyl-L-leucine + H2O
-
 Pseudomonas putida 4-hydroxybenzoic acid + L-histidyl-L-leucine
-
 ?
4-nitro-N-benzoyl-L-alanine + H2O
-
 Pseudomonas putida 4-nitrobenzoic acid + L-alanine
-
 ?
4-nitro-N-benzoylglycine + H2O best substrate Pseudomonas putida 4-nitrobenzoic acid + glycine
-
 ?
N-(4-nitrobenzoyl)-Gly-Gly-Gly + H2O
-
 Pseudomonas putida 4-nitrobenzoic acid + Gly-Gly-Gly
-
 ?
N-benzoyl-DL-methionine + H2O poor substrate Pseudomonas putida benzoic acid + DL-methionine
-
 ?
N-benzoyl-L-alanine + H2O
-
 Pseudomonas putida benzoic acid + L-alanine
-
 ?
N-benzoyl-L-aminobutyric acid + H2O low activity Pseudomonas putida benzoic acid + L-aminobutyric acid
-
 ?
N-benzoyl-L-valine + H2O poor substrate Pseudomonas putida benzoic acid + L-valine
-
 ?
N-benzoylglycine + H2O hippurate Pseudomonas putida benzoic acid + glycine
-
 ?
N-benzoylglycylglycylglycine + H2O
-
 Pseudomonas putida benzoic acid + glycylglycylglycine
-
 ?
N-[(4-hydroxyphenyl)carbonyl]glycine + H2O
-
 Pseudomonas putida ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 42000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 stable between, labile in acidic pH Pseudomonas putida