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Literature summary for 3.5.1.30 extracted from

  • Kim, H.T.; Khang, T.U.; Baritugo, K.A.; Hyun, S.M.; Kang, K.H.; Jung, S.H.; Song, B.K.; Park, K.; Oh, M.K.; Kim, G.B.; Kim, H.U.; Lee, S.Y.; Park, S.J.; Joo, J.C.
    Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical (2019), Metab. Eng., 51, 99-109 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene davA, coexpression with Pseudomonas putida genes davB, davT, and davD in Corynebacterium glutamicum engineered strain H30_GAHis under control of strong synthetic promoters PH30 and PH36, fed-batch fermentation Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information Corynebacterium glutamicum KCTC 1857, which naturally producs high amounts of L-lysine, a direct precursor for the production of cadaverine, 5-AVA, and glutaric acid, is metabolically engineered for the production of glutaric acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons and plasticizers. Corynebacterium glutamicum gabT and gabD genes and Pseudomonas putida davT and davD genes encoding 5-aminovalerate transaminase and glutarate semialdehyde dehydrogenase, respectively, are examined in Corynebacterium glutamicum for the construction of a glutaric acid biosynthesis pathway along with Pseudomonas putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. Evaluation of strong synthetic promoters PH30 and PH36, usage of Corynebacterium glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His6-tag are established to improve the production of glutaric acid. N-terminal His6-tagged DavB is most suitable for the production of glutaric acid from glucose. Fed-batch fermentation using the final engineered Corynebacterium glutamicum H30_GAHis strain, expressing davTDA genes along with N-terminally His6-tagged davB produce 24.5 g/l of glutaric acid with low accumulation of L-lysine (1.7 g/l), wherein 5-AVA accumulation is not observed during fermentation Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-aminopentanamide + H2O Pseudomonas putida
-
 5-aminopentanoate + NH3
-
 ?
5-aminopentanamide + H2O Pseudomonas putida ATCC 12633
-
 5-aminopentanoate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida B3IVI7
-
-
Pseudomonas putida ATCC 12633 B3IVI7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-aminopentanamide + H2O
-
 Pseudomonas putida 5-aminopentanoate + NH3
-
 ?
5-aminopentanamide + H2O
-
 Pseudomonas putida ATCC 12633 5-aminopentanoate + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Pseudomonas putida