Cloned (Comment) | Organism |
---|---|
gene davA, recombinant expression in Corynebacterium glutamicum, coexpression with gene davB from Pseudomonas putida encoding lysine 2-monooxygenase (EC 1.13.12.2) | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | systems metabolic engineering of Corynebacterium glutamicum for construction of a recombinant microbial cell factory for the production of the carbon-5 platform chemicals 5-aminovalerate and glutarate, method development on basis of the lysine-hyperproducing strain Corynebacterium glutamicum LYS-12 and optimization, detailed overview. Related to the presence of endogenous genes coding for 5-aminovalerate transaminase (gabT) and glutarate semialdehyde dehydrogenase, 5-aminovalerate is partially converted to glutarate. Moreover, residual l-lysine is secreted as by-product. The issue of by-product formation is then addressed by deletion of the lysE gene, encoding the l-lysine exporter. Additionally, a putative gabT gene is deleted to enhance 5-aminovalerate production. Fed-batch fermentation. Method optimization, overview. Production of 28 g/l of 5-aminovalerate with a maximal space to time yield of 0.9 g/l per h from engineered AVA-3 strain | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminopentanamide + H2O | Pseudomonas putida | - |
5-aminopentanoate + NH3 | - |
? | |
5-aminopentanamide + H2O | Pseudomonas putida ATCC 12633 | - |
5-aminopentanoate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | B3IVI7 | - |
- |
Pseudomonas putida ATCC 12633 | B3IVI7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminopentanamide + H2O | - |
Pseudomonas putida | 5-aminopentanoate + NH3 | - |
? | |
5-aminopentanamide + H2O | - |
Pseudomonas putida ATCC 12633 | 5-aminopentanoate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-aminovaleramidase | - |
Pseudomonas putida |
5-aminovaleramide amidase | - |
Pseudomonas putida |
DavA | - |
Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
metabolism | metabolic pathway design for the recombinant production of 5-aminovalerate and glutarate in Corynebacterium glutamicum starting from the pathway precursor L-lysine and involving 5-aminovaleramide amidase, overview | Pseudomonas putida |