BRENDA - Enzyme Database show
show all sequences of 3.5.1.30

delta-Aminovaleramidase of Pseudomonas putida

Reitz, M.S.; Rodwell, V.W.; J. Biol. Chem. 245, 3091-3096 (1970)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithioerythritol
effectively reverses heavy metal inactivation
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
AgNO3
0.00125 mM, 100% inhibition
Pseudomonas putida
arsenite
characteristic of enzymes with essential vicinal sulfhydryl groups
Pseudomonas putida
Ba2+
5 mM, inhibition from 10-40%
Pseudomonas putida
Ca2+
5 mM, inhibition from 10-40%
Pseudomonas putida
Cd(acetate)2
0.00125 mM, 94% inhibition
Pseudomonas putida
Cd2+
0.5 mM, complete inhibition , characteristic of enzymes with essential vicinal sulfhydryl groups
Pseudomonas putida
Co2+
5.0 mM,75% inhibition
Pseudomonas putida
Cu2+
5.0 mM, complete inhibition
Pseudomonas putida
Fe2+
5 mM, inhibition from 10-40%
Pseudomonas putida
Hg(NO3)2
0.187 mM, 100% inhibition
Pseudomonas putida
Hg2+
0.5 mM, complete inhibition
Pseudomonas putida
HgCl2
0.00125 mM, 100% inhibition
Pseudomonas putida
Mg2+
5 mM, inhibition from 10-40%
Pseudomonas putida
NaAsO2
0.0125 mM, 47% inhibition
Pseudomonas putida
p-hydroxymercuribenzoate
0.00025 mM, 44% inhibition, inhibition partially reversed by dithioerythritol
Pseudomonas putida
Pb2+
5.0 mM, complete inhibition
Pseudomonas putida
Sn2+
5.0 mM, complete inhibition
Pseudomonas putida
Zn2+
0.5 mM, complete inhibition
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
12
-
6-Aminohexanamide
-
Pseudomonas putida
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pseudomonas putida
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Al3+
0.125 mM stimulates activity 37%
Pseudomonas putida
Fe3+
0.125 mM stimulates activity 25%
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-aminopentanamide + H2O
Pseudomonas putida
known intermediate in lysine catabolism
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
purified 400fold from the soluble fraction of cell-free extract
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
130
-
-
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-aminopentanamide + H2O
-
172031
Pseudomonas putida
5-aminopentanoate + NH3
-
-
-
?
5-aminopentanamide + H2O
known intermediate in lysine catabolism
172031
Pseudomonas putida
?
-
-
-
-
6-aminohexanamide + H2O
-
172031
Pseudomonas putida
6-aminohexanoate + NH3
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Pseudomonas putida
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
stable below pH 6.5, irreversibly inactivated above pH 9
Pseudomonas putida
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithioerythritol
effectively reverses heavy metal inactivation
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AgNO3
0.00125 mM, 100% inhibition
Pseudomonas putida
arsenite
characteristic of enzymes with essential vicinal sulfhydryl groups
Pseudomonas putida
Ba2+
5 mM, inhibition from 10-40%
Pseudomonas putida
Ca2+
5 mM, inhibition from 10-40%
Pseudomonas putida
Cd(acetate)2
0.00125 mM, 94% inhibition
Pseudomonas putida
Cd2+
0.5 mM, complete inhibition , characteristic of enzymes with essential vicinal sulfhydryl groups
Pseudomonas putida
Co2+
5.0 mM,75% inhibition
Pseudomonas putida
Cu2+
5.0 mM, complete inhibition
Pseudomonas putida
Fe2+
5 mM, inhibition from 10-40%
Pseudomonas putida
Hg(NO3)2
0.187 mM, 100% inhibition
Pseudomonas putida
Hg2+
0.5 mM, complete inhibition
Pseudomonas putida
HgCl2
0.00125 mM, 100% inhibition
Pseudomonas putida
Mg2+
5 mM, inhibition from 10-40%
Pseudomonas putida
NaAsO2
0.0125 mM, 47% inhibition
Pseudomonas putida
p-hydroxymercuribenzoate
0.00025 mM, 44% inhibition, inhibition partially reversed by dithioerythritol
Pseudomonas putida
Pb2+
5.0 mM, complete inhibition
Pseudomonas putida
Sn2+
5.0 mM, complete inhibition
Pseudomonas putida
Zn2+
0.5 mM, complete inhibition
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
12
-
6-Aminohexanamide
-
Pseudomonas putida
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pseudomonas putida
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Al3+
0.125 mM stimulates activity 37%
Pseudomonas putida
Fe3+
0.125 mM stimulates activity 25%
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-aminopentanamide + H2O
Pseudomonas putida
known intermediate in lysine catabolism
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
purified 400fold from the soluble fraction of cell-free extract
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
130
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-aminopentanamide + H2O
-
172031
Pseudomonas putida
5-aminopentanoate + NH3
-
-
-
?
5-aminopentanamide + H2O
known intermediate in lysine catabolism
172031
Pseudomonas putida
?
-
-
-
-
6-aminohexanamide + H2O
-
172031
Pseudomonas putida
6-aminohexanoate + NH3
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Pseudomonas putida
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
stable below pH 6.5, irreversibly inactivated above pH 9
Pseudomonas putida
Other publictions for EC 3.5.1.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728239
Park
Metabolic engineering of Esche ...
Pseudomonas putida, Pseudomonas putida ATCC 12633
Metab. Eng.
16
42-47
2013
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
172030
Reitz
-
delta-Aminovaleramidase (Pseud ...
Pseudomonas putida, Pseudomonas putida P2-ATCC *25571
Methods Enzymol.
17
158-165
1971
1
-
-
-
-
-
3
3
1
-
-
2
-
2
-
-
1
-
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
3
1
-
-
2
-
-
-
1
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
172031
Reitz
delta-Aminovaleramidase of Pse ...
Pseudomonas putida
J. Biol. Chem.
245
3091-3096
1970
1
-
-
-
-
-
18
1
1
2
1
1
-
2
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
18
-
1
1
2
1
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-