BRENDA - Enzyme Database show
show all sequences of 3.5.1.30

delta-Aminovaleramidase (Pseudomonas putida)

Reitz, M.S.; Rodwell, V.W.; Methods Enzymol. 17, 158-165 (1971)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
L-lysine
induces enzyme
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
-
Pseudomonas putida
HgCl2
-
Pseudomonas putida
p-hydroxymercuribenzoate
-
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
5-Aminopentanamide
-
Pseudomonas putida
4.1
-
gamma-aminobutyramide
-
Pseudomonas putida
6
-
methyl-delta-aminovalerate
-
Pseudomonas putida
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pseudomonas putida
5737
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-aminopentanamide + H2O
Pseudomonas putida
-
?
-
-
-
5-aminopentanamide + H2O
Pseudomonas putida P2-ATCC *25571
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
P2-ATCC*25571
-
Pseudomonas putida P2-ATCC *25571
-
P2-ATCC*25571
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
130
-
-
Pseudomonas putida
Storage Stability
Storage Stability
Organism
EDTA and dithioerythritol required for optimum stability, enzyme stored in their absence rapidly deteriorates
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutyramide + H2O
-
172030
Pseudomonas putida
4-aminobutanoate + NH3
-
-
-
?
4-aminobutyramide + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
4-aminobutanoate + NH3
-
-
-
?
5-aminopentanamide + H2O
-
172030
Pseudomonas putida
5-aminopentanoate + NH3
-
-
-
?
5-aminopentanamide + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
5-aminopentanoate + NH3
-
-
-
?
5-aminopentanamide + H2O
-
172030
Pseudomonas putida
?
-
-
-
-
5-aminopentanamide + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
?
-
-
-
-
6-aminohexanamide + H2O
-
172030
Pseudomonas putida
6-aminohexanoate + NH3
-
-
-
?
6-aminohexanamide + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
6-aminohexanoate + NH3
-
-
-
?
methyl-delta-aminovalerate + H2O
-
172030
Pseudomonas putida
5-aminopentanoate + methanol
-
-
-
?
methyl-delta-aminovalerate + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
5-aminopentanoate + methanol
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Pseudomonas putida
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
L-lysine
induces enzyme
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
-
Pseudomonas putida
HgCl2
-
Pseudomonas putida
p-hydroxymercuribenzoate
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
5-Aminopentanamide
-
Pseudomonas putida
4.1
-
gamma-aminobutyramide
-
Pseudomonas putida
6
-
methyl-delta-aminovalerate
-
Pseudomonas putida
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pseudomonas putida
5737
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-aminopentanamide + H2O
Pseudomonas putida
-
?
-
-
-
5-aminopentanamide + H2O
Pseudomonas putida P2-ATCC *25571
-
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
130
-
-
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
EDTA and dithioerythritol required for optimum stability, enzyme stored in their absence rapidly deteriorates
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutyramide + H2O
-
172030
Pseudomonas putida
4-aminobutanoate + NH3
-
-
-
?
4-aminobutyramide + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
4-aminobutanoate + NH3
-
-
-
?
5-aminopentanamide + H2O
-
172030
Pseudomonas putida
5-aminopentanoate + NH3
-
-
-
?
5-aminopentanamide + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
5-aminopentanoate + NH3
-
-
-
?
5-aminopentanamide + H2O
-
172030
Pseudomonas putida
?
-
-
-
-
5-aminopentanamide + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
?
-
-
-
-
6-aminohexanamide + H2O
-
172030
Pseudomonas putida
6-aminohexanoate + NH3
-
-
-
?
6-aminohexanamide + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
6-aminohexanoate + NH3
-
-
-
?
methyl-delta-aminovalerate + H2O
-
172030
Pseudomonas putida
5-aminopentanoate + methanol
-
-
-
?
methyl-delta-aminovalerate + H2O
-
172030
Pseudomonas putida P2-ATCC *25571
5-aminopentanoate + methanol
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Pseudomonas putida
Other publictions for EC 3.5.1.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728239
Park
Metabolic engineering of Esche ...
Pseudomonas putida, Pseudomonas putida ATCC 12633
Metab. Eng.
16
42-47
2013
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
172030
Reitz
-
delta-Aminovaleramidase (Pseud ...
Pseudomonas putida, Pseudomonas putida P2-ATCC *25571
Methods Enzymol.
17
158-165
1971
1
-
-
-
-
-
3
3
1
-
-
2
-
2
-
-
1
-
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
3
1
-
-
2
-
-
-
1
-
-
1
1
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
172031
Reitz
delta-Aminovaleramidase of Pse ...
Pseudomonas putida
J. Biol. Chem.
245
3091-3096
1970
1
-
-
-
-
-
18
1
1
2
1
1
-
2
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
18
-
1
1
2
1
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-