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Literature summary for 3.5.1.3 extracted from
- Assay and purification of omega-amidase/Nit2, a ubiquitously expressed putative tumor suppressor, that catalyzes the deamidation of the alpha-keto acid analogues of glutamine and asparagine (2009), Anal. Biochem., 391, 144-150.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | development of a method to synthesize 2-oxoglutaramate which is not commercially available and development of an enzyme assay method to measure the hydrolysis of 2-oxoglutaramate and 2-oxosuccinamate in a 96-well plate format | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, dialysis, DE-52 chromatography, hydroxylapatite chromatography, purified 3600fold, 15.1% recovery | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 126 | - |
2-oxoglutaramate as substrate, pH 8.5, 37°C | Rattus norvegicus |
| 570 | - |
2-oxosuccinamate as substrate, pH 8.5, 37°C | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutaramate + H2O | enzyme activity measured in a 96-well plate format | Rattus norvegicus | 2-oxoglutarate + NH3 | - |
? |  |
| 2-oxosuccinamate + H2O | enzyme activity measured in a 96-well plate format | Rattus norvegicus | oxaloacetate + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| omega-amidase/Nit2 | omega-amidase has been shown to be identical to Nit2, a putative tumor suppressor protein | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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