Application | Comment | Organism |
---|---|---|
biotechnology | development of a method to synthesize 2-oxoglutaramate which is not commercially available and development of an enzyme assay method to measure the hydrolysis of 2-oxoglutaramate and 2-oxosuccinamate in a 96-well plate format | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, dialysis, DE-52 chromatography, hydroxylapatite chromatography, purified 3600fold, 15.1% recovery | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
126 | - |
2-oxoglutaramate as substrate, pH 8.5, 37°C | Rattus norvegicus |
570 | - |
2-oxosuccinamate as substrate, pH 8.5, 37°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutaramate + H2O | enzyme activity measured in a 96-well plate format | Rattus norvegicus | 2-oxoglutarate + NH3 | - |
? | |
2-oxosuccinamate + H2O | enzyme activity measured in a 96-well plate format | Rattus norvegicus | oxaloacetate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
omega-amidase/Nit2 | omega-amidase has been shown to be identical to Nit2, a putative tumor suppressor protein | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Rattus norvegicus |