BRENDA - Enzyme Database show
show all sequences of 3.5.1.3

Rat liver omega-amidase. Purofication and properties

Hersh, L.B.; Biochemistry 10, 2884-2891 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-(2-nitrobenzoic acid)
0.02 mM: complete inhibition; Ellman's reagent
Rattus norvegicus
ethanol
0.05 M: no inhibition
Rattus norvegicus
Glutaramate
competitive inhibition, 10 mM glutaramate, hydrolysis of methyl alpha-ketoglutarate: 43% inhibition, hydrolysis of ethyl alpha-ketoglutarate: 34% inhibition
Rattus norvegicus
iodoacetamide
0.2 mM: complete inhibition
Rattus norvegicus
iodoacetate
0.2 mM: complete inhibition
Rattus norvegicus
methylamine
0.05 M methylamine: 62% inhibition
Rattus norvegicus
additional information
no inhibition by 0.05 M methanol, ethanol, propanol, ethylamine, propylamine, butylamine, dimethylamine, dierhylamine and triethylamine; no inhibition by EDTA
Rattus norvegicus
N-ethylmaleimide
0.2 mM: complete inhibition
Rattus norvegicus
NH4+
0.05 M ammonia: 72% inhibition
Rattus norvegicus
p-mercuribenzoate
0.02 mM: complete inhibition
Rattus norvegicus
succinamate
competitive inhibition, 5 mM succinamate, hydrolysis of methyl alpha-ketoglutarate: 57% inhibition, hydrolysis of ethyl alpha-ketoglutarate: 42% inhibition
Rattus norvegicus
sulfhydryl reagents
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic studies, criteria and data
Rattus norvegicus
0.01
-
2-Oxoglutaramate
true Km of omega-amidase for alpha-ketoglutaramate open-chain structure may be of the order of 0.01 mM
Rattus norvegicus
0.03
-
gamma-methyl 2-oxoglutarate
hydrolysis
Rattus norvegicus
0.06
-
gamma-ethyl 2-oxoglutarate
hydrolysis
Rattus norvegicus
0.2
-
succinamate
hydrolysis
Rattus norvegicus
0.7
-
methyl succinate
hydrolysis
Rattus norvegicus
0.8
-
succinyl hydroxamate
hydrolysis
Rattus norvegicus
1.8
-
methyl glutarate
hydrolysis
Rattus norvegicus
3.1
-
Glutaramate
hydrolysis
Rattus norvegicus
3.3
-
2-oxoglutarate
hydrolysis
Rattus norvegicus
3.6
-
hydroxylamine
hydroxaminolysis, substrate: methyl glutarate
Rattus norvegicus
4
-
hydroxylamine
hydroxaminolysis, substrate: glutaramate
Rattus norvegicus
5.4
-
hydroxylamine
hydroxaminolysis, substrate: succinamate
Rattus norvegicus
6.8
-
propyl glutarate
hydrolysis
Rattus norvegicus
8
-
methyl succinate
hydroxaminolysis
Rattus norvegicus
8.3
-
ethyl succinate
hydrolysis
Rattus norvegicus
9.9
-
ethyl glutarate
hydrolysis
Rattus norvegicus
17
-
ethyl succinate
hydroxaminolysis
Rattus norvegicus
17
-
succinamate
hydroxaminolysis
Rattus norvegicus
18
-
propyl glutarate
hydroxaminolysis
Rattus norvegicus
25
-
Glutaramate
hydroxaminolysis
Rattus norvegicus
31
-
ethyl glutarate
hydroxaminolysis
Rattus norvegicus
52
-
methyl glutarate
hydroxaminolysis
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no effect of 0.05 M NaCl, KCl, MgCl2, CaCl2, or BaCl2
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
liver, high-speed sedimentation equilibrium and amino-acid analysis
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoglutaramate + H2O
Rattus norvegicus
alpha-ketoglutaramate
2-oxoglutarate + NH3
-
Rattus norvegicus
?
additional information
Rattus norvegicus
conversion of glutamine into alpha-ketoglutarate and ammonia is brought about by two distinct enzymes: glutamine transaminase and omega-amidase
?
-
-
-
additional information
Rattus norvegicus
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
?
-
-
-
additional information
Rattus norvegicus
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Reaction
Reaction
Commentary
Organism
a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3
mechanism
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rattus norvegicus
11.7
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
3°C, 1 mM dithiothreitol, 2 weeks, less than 10% activity lost
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutaramate + H2O
alpha-ketoglutaramate
288892
Rattus norvegicus
2-oxoglutarate + NH3
-
288892
Rattus norvegicus
?
2-oxoglutaramate + hydroxylamine
no hydroxaminolysis of alpha-ketoglutaramate at pH 8
288892
Rattus norvegicus
2-oxoglutaryl hydroxamate + NH3
-
-
-
-
2-oxoglutaramate + methylamine
alpha-ketoglutaramate
288892
Rattus norvegicus
?
-
-
-
?
2-oxoglutaramate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
2-oxosuccinamate + H2O
alpha-ketosuccinamate
288892
Rattus norvegicus
2-oxosuccinate + NH3
-
-
-
?
butyl glutarate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaramate + butanol
-
-
-
?
delta-ethyl 2-oxoglutarate + H2O
delta-ethyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
delta-methyl 2-oxoglutarate + H2O
delta-methyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
ethyl glutarate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
ethyl glutarate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaramate + ethanol
-
-
-
?
ethyl glutarate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
ethyl succinate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
ethyl succinate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
succinamate + ethanol
-
-
-
?
ethyl succinate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
gamma-ethyl 2-oxoglutarate + H2O
gamma-methyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
gamma-ethyl 2-oxoglutarate + methylamine
transamidation, poor
288892
Rattus norvegicus
?
-
-
-
?
gamma-ethyl 2-oxoglutarate + methylamine
gamma-ethyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
gamma-methyl 2-oxoglutarate + H2O
gamma-methyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
gamma-methyl 2-oxoglutarate + methylamine
transamidation, very poor
288892
Rattus norvegicus
?
-
-
-
?
glutaramate + H2O
-
288892
Rattus norvegicus
glutarate + NH3
-
-
-
?
glutaramate + hydroxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaryl hydroxamate + NH3
-
-
-
?
glutaramate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
glutarate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
methyl glutarate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
methyl glutarate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaramate + methanol
-
-
-
?
methyl glutarate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
methyl succinate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
methyl succinate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
succinamate + methanol
-
-
-
?
methyl succinate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
additional information
broad specificity
288892
Rattus norvegicus
?
-
-
-
-
additional information
substrate specificity
288892
Rattus norvegicus
?
-
-
-
-
additional information
no hydrolysis of glutamine, asparagine, N-methyl-alpha-ketoglutaramate, dimethyl glutarate, or the anilides of succinate or glutarate. No hydrolysis of N-methylsuccinamate or N-ethylsuccinamate
288892
Rattus norvegicus
?
-
-
-
-
additional information
enzyme has esterolytic activity
288892
Rattus norvegicus
?
-
-
-
-
additional information
hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, gamma-ester, glutarate, succinate, and p-chloro, p-methyl, and unsubstituted phenyl esters of glutarate
288892
Rattus norvegicus
?
-
-
-
-
additional information
hydrolyzes monomethyl and ethyl esters of alpha-ketoglutarate, delta esters, succinate, and glutarate
288892
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes methylamide formation
288892
Rattus norvegicus
?
-
-
-
-
additional information
no hydroxaminolysis of delta-methyl glutamate, delta-ethyl glutamate, malonate, glutamate, glutarate, aspartate, succinate, glutamine, asparagine, succinanilate, glutaranilate, alpha-ketoglutaramate N-methylsuccinamate, and N-ethylsuccinamate at pH 8
288892
Rattus norvegicus
?
-
-
-
-
additional information
hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, delta-ester, glutarate, and succinate, and monopropyl ester of glutarate
288892
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes hydroxaminolysis and transamidation reactions with esters and amides
288892
Rattus norvegicus
?
-
-
-
-
additional information
conversion of glutamine into alpha-ketoglutarate and ammonia is brought about by two distinct enzymes: glutamine transaminase and omega-amidase
288892
Rattus norvegicus
?
-
-
-
-
additional information
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
288892
Rattus norvegicus
?
-
-
-
-
additional information
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
288892
Rattus norvegicus
?
-
-
-
-
propyl glutarate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
propyl glutarate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaramate + propanol
-
-
-
?
succinamate + H2O
-
288892
Rattus norvegicus
succinic acid + NH3
-
-
-
?
succinamate + hydroxylamine
hydroxaminolysis
288892
Rattus norvegicus
succinyl hydroxamate + NH3
-
-
-
r
succinamate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
succinate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
succinyl hydroxamate + H2O
-
288892
Rattus norvegicus
succinate + hydroxylamine
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
liver, 2 * 27000-28000, two subunits may be nonidentical, high-speed sedimentation equilibrium in the presence of 7 M guanidine hydrochloride or 7 M urea containing 0.1 M 2-mercaptoethanol
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rattus norvegicus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
30 min, 0.02 M Tris-HCl, pH 8.5, no loss of activity
Rattus norvegicus
60
-
5 min, 70% loss of activity
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Rattus norvegicus
8.5
-
assay at
Rattus norvegicus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
-
Rattus norvegicus
4
-
20 min, 90% loss of activity
Rattus norvegicus
5
-
20 min, 32% loss of activity
Rattus norvegicus
6
10
20 min, 30°C, stable between, no irreversible inactivation
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-(2-nitrobenzoic acid)
0.02 mM: complete inhibition; Ellman's reagent
Rattus norvegicus
ethanol
0.05 M: no inhibition
Rattus norvegicus
Glutaramate
competitive inhibition, 10 mM glutaramate, hydrolysis of methyl alpha-ketoglutarate: 43% inhibition, hydrolysis of ethyl alpha-ketoglutarate: 34% inhibition
Rattus norvegicus
iodoacetamide
0.2 mM: complete inhibition
Rattus norvegicus
iodoacetate
0.2 mM: complete inhibition
Rattus norvegicus
methylamine
0.05 M methylamine: 62% inhibition
Rattus norvegicus
additional information
no inhibition by 0.05 M methanol, ethanol, propanol, ethylamine, propylamine, butylamine, dimethylamine, dierhylamine and triethylamine; no inhibition by EDTA
Rattus norvegicus
N-ethylmaleimide
0.2 mM: complete inhibition
Rattus norvegicus
NH4+
0.05 M ammonia: 72% inhibition
Rattus norvegicus
p-mercuribenzoate
0.02 mM: complete inhibition
Rattus norvegicus
succinamate
competitive inhibition, 5 mM succinamate, hydrolysis of methyl alpha-ketoglutarate: 57% inhibition, hydrolysis of ethyl alpha-ketoglutarate: 42% inhibition
Rattus norvegicus
sulfhydryl reagents
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic studies, criteria and data
Rattus norvegicus
0.01
-
2-Oxoglutaramate
true Km of omega-amidase for alpha-ketoglutaramate open-chain structure may be of the order of 0.01 mM
Rattus norvegicus
0.03
-
gamma-methyl 2-oxoglutarate
hydrolysis
Rattus norvegicus
0.06
-
gamma-ethyl 2-oxoglutarate
hydrolysis
Rattus norvegicus
0.2
-
succinamate
hydrolysis
Rattus norvegicus
0.7
-
methyl succinate
hydrolysis
Rattus norvegicus
0.8
-
succinyl hydroxamate
hydrolysis
Rattus norvegicus
1.8
-
methyl glutarate
hydrolysis
Rattus norvegicus
3.1
-
Glutaramate
hydrolysis
Rattus norvegicus
3.3
-
2-oxoglutarate
hydrolysis
Rattus norvegicus
3.6
-
hydroxylamine
hydroxaminolysis, substrate: methyl glutarate
Rattus norvegicus
4
-
hydroxylamine
hydroxaminolysis, substrate: glutaramate
Rattus norvegicus
5.4
-
hydroxylamine
hydroxaminolysis, substrate: succinamate
Rattus norvegicus
6.8
-
propyl glutarate
hydrolysis
Rattus norvegicus
8
-
methyl succinate
hydroxaminolysis
Rattus norvegicus
8.3
-
ethyl succinate
hydrolysis
Rattus norvegicus
9.9
-
ethyl glutarate
hydrolysis
Rattus norvegicus
17
-
ethyl succinate
hydroxaminolysis
Rattus norvegicus
17
-
succinamate
hydroxaminolysis
Rattus norvegicus
18
-
propyl glutarate
hydroxaminolysis
Rattus norvegicus
25
-
Glutaramate
hydroxaminolysis
Rattus norvegicus
31
-
ethyl glutarate
hydroxaminolysis
Rattus norvegicus
52
-
methyl glutarate
hydroxaminolysis
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no effect of 0.05 M NaCl, KCl, MgCl2, CaCl2, or BaCl2
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
liver, high-speed sedimentation equilibrium and amino-acid analysis
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoglutaramate + H2O
Rattus norvegicus
alpha-ketoglutaramate
2-oxoglutarate + NH3
-
Rattus norvegicus
?
additional information
Rattus norvegicus
conversion of glutamine into alpha-ketoglutarate and ammonia is brought about by two distinct enzymes: glutamine transaminase and omega-amidase
?
-
-
-
additional information
Rattus norvegicus
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
?
-
-
-
additional information
Rattus norvegicus
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rattus norvegicus
11.7
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
3°C, 1 mM dithiothreitol, 2 weeks, less than 10% activity lost
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutaramate + H2O
alpha-ketoglutaramate
288892
Rattus norvegicus
2-oxoglutarate + NH3
-
288892
Rattus norvegicus
?
2-oxoglutaramate + hydroxylamine
no hydroxaminolysis of alpha-ketoglutaramate at pH 8
288892
Rattus norvegicus
2-oxoglutaryl hydroxamate + NH3
-
-
-
-
2-oxoglutaramate + methylamine
alpha-ketoglutaramate
288892
Rattus norvegicus
?
-
-
-
?
2-oxoglutaramate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
2-oxosuccinamate + H2O
alpha-ketosuccinamate
288892
Rattus norvegicus
2-oxosuccinate + NH3
-
-
-
?
butyl glutarate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaramate + butanol
-
-
-
?
delta-ethyl 2-oxoglutarate + H2O
delta-ethyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
delta-methyl 2-oxoglutarate + H2O
delta-methyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
ethyl glutarate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
ethyl glutarate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaramate + ethanol
-
-
-
?
ethyl glutarate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
ethyl succinate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
ethyl succinate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
succinamate + ethanol
-
-
-
?
ethyl succinate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
gamma-ethyl 2-oxoglutarate + H2O
gamma-methyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
gamma-ethyl 2-oxoglutarate + methylamine
transamidation, poor
288892
Rattus norvegicus
?
-
-
-
?
gamma-ethyl 2-oxoglutarate + methylamine
gamma-ethyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
gamma-methyl 2-oxoglutarate + H2O
gamma-methyl alpha-ketoglutarate
288892
Rattus norvegicus
?
-
-
-
?
gamma-methyl 2-oxoglutarate + methylamine
transamidation, very poor
288892
Rattus norvegicus
?
-
-
-
?
glutaramate + H2O
-
288892
Rattus norvegicus
glutarate + NH3
-
-
-
?
glutaramate + hydroxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaryl hydroxamate + NH3
-
-
-
?
glutaramate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
glutarate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
methyl glutarate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
methyl glutarate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaramate + methanol
-
-
-
?
methyl glutarate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
methyl succinate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
methyl succinate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
succinamate + methanol
-
-
-
?
methyl succinate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
additional information
broad specificity
288892
Rattus norvegicus
?
-
-
-
-
additional information
substrate specificity
288892
Rattus norvegicus
?
-
-
-
-
additional information
no hydrolysis of glutamine, asparagine, N-methyl-alpha-ketoglutaramate, dimethyl glutarate, or the anilides of succinate or glutarate. No hydrolysis of N-methylsuccinamate or N-ethylsuccinamate
288892
Rattus norvegicus
?
-
-
-
-
additional information
enzyme has esterolytic activity
288892
Rattus norvegicus
?
-
-
-
-
additional information
hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, gamma-ester, glutarate, succinate, and p-chloro, p-methyl, and unsubstituted phenyl esters of glutarate
288892
Rattus norvegicus
?
-
-
-
-
additional information
hydrolyzes monomethyl and ethyl esters of alpha-ketoglutarate, delta esters, succinate, and glutarate
288892
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes methylamide formation
288892
Rattus norvegicus
?
-
-
-
-
additional information
no hydroxaminolysis of delta-methyl glutamate, delta-ethyl glutamate, malonate, glutamate, glutarate, aspartate, succinate, glutamine, asparagine, succinanilate, glutaranilate, alpha-ketoglutaramate N-methylsuccinamate, and N-ethylsuccinamate at pH 8
288892
Rattus norvegicus
?
-
-
-
-
additional information
hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, delta-ester, glutarate, and succinate, and monopropyl ester of glutarate
288892
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes hydroxaminolysis and transamidation reactions with esters and amides
288892
Rattus norvegicus
?
-
-
-
-
additional information
conversion of glutamine into alpha-ketoglutarate and ammonia is brought about by two distinct enzymes: glutamine transaminase and omega-amidase
288892
Rattus norvegicus
?
-
-
-
-
additional information
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
288892
Rattus norvegicus
?
-
-
-
-
additional information
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
288892
Rattus norvegicus
?
-
-
-
-
propyl glutarate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
propyl glutarate + hydoxylamine
hydroxaminolysis
288892
Rattus norvegicus
glutaramate + propanol
-
-
-
?
succinamate + H2O
-
288892
Rattus norvegicus
succinic acid + NH3
-
-
-
?
succinamate + hydroxylamine
hydroxaminolysis
288892
Rattus norvegicus
succinyl hydroxamate + NH3
-
-
-
r
succinamate + methylamine
transamidation
288892
Rattus norvegicus
?
-
-
-
?
succinate + H2O
-
288892
Rattus norvegicus
?
-
-
-
?
succinyl hydroxamate + H2O
-
288892
Rattus norvegicus
succinate + hydroxylamine
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
liver, 2 * 27000-28000, two subunits may be nonidentical, high-speed sedimentation equilibrium in the presence of 7 M guanidine hydrochloride or 7 M urea containing 0.1 M 2-mercaptoethanol
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rattus norvegicus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
30 min, 0.02 M Tris-HCl, pH 8.5, no loss of activity
Rattus norvegicus
60
-
5 min, 70% loss of activity
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Rattus norvegicus
8.5
-
assay at
Rattus norvegicus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
-
Rattus norvegicus
4
-
20 min, 90% loss of activity
Rattus norvegicus
5
-
20 min, 32% loss of activity
Rattus norvegicus
6
10
20 min, 30°C, stable between, no irreversible inactivation
Rattus norvegicus
Other publictions for EC 3.5.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734871
Zhang
Identification and characteriz ...
Arabidopsis thaliana, Arabidopsis thaliana Col
Phytochemistry
99
36-43
2014
-
-
1
-
-
-
-
3
2
-
2
2
-
5
-
-
1
-
-
1
-
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
2
-
2
2
-
-
-
1
-
1
-
-
10
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
721018
Cobzaru
Homologous gene clusters of ni ...
Nocardioides sp., Nocardioides sp. JS614 / ATCC BAA-499, Paenarthrobacter nicotinovorans, Rhodococcus opacus
Res. Microbiol.
162
285-291
2011
-
-
2
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
5
-
3
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
5
-
3
-
-
-
3
-
-
-
-
6
6
-
-
-
710840
Krasnikov
Assay and purification of omeg ...
Rattus norvegicus
Anal. Biochem.
391
144-150
2009
-
1
-
-
-
-
-
-
4
-
1
-
-
4
-
-
1
-
-
4
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
1
-
4
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
711316
Jaisson
Molecular identification of om ...
Mus musculus
Biochimie
91
1066-1071
2009
-
-
1
-
-
-
-
8
-
-
-
-
-
5
-
-
1
-
-
1
-
-
6
-
1
-
-
8
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
1
-
1
-
-
6
-
1
-
-
8
2
-
-
-
-
-
-
-
8
8
711317
Krasnikov
Identification of the putative ...
Homo sapiens, Rattus norvegicus
Biochimie
91
1072-1080
2009
-
-
1
-
-
-
-
3
-
-
8
4
-
4
-
-
2
-
-
3
-
-
4
2
2
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
8
4
-
-
-
2
-
3
-
-
4
2
2
-
-
-
2
-
-
-
-
1
1
-
-
-
288890
Makar
Glutamine transaminase K and o ...
Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus
J. Neurochem.
62
1983-1988
1994
-
-
-
-
-
-
-
-
-
-
-
8
-
8
-
-
-
-
-
20
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
20
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288891
Cooper
High activities of glutamine t ...
Rattus norvegicus
J. Neurochem.
61
1731-1741
1993
-
-
-
-
-
-
-
-
3
-
-
5
-
4
-
-
-
-
-
7
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
5
-
-
-
-
-
7
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288884
Cooper
alpha-Keto acid omega-amidase ...
Embryophyta, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae
Methods Enzymol.
113
350-358
1985
-
3
-
-
-
2
8
3
5
1
2
8
-
6
1
-
1
1
1
13
2
2
24
1
-
-
3
-
2
-
-
-
-
-
-
-
3
-
-
-
-
2
-
8
-
3
5
1
2
8
-
1
-
1
1
13
2
2
24
1
-
-
3
-
2
-
-
-
-
-
-
-
-
-
288885
Calderon
omega-Amidase pathway in the d ...
Neurospora crassa
J. Bacteriol.
161
807-809
1985
-
-
-
-
-
-
3
-
-
-
-
2
-
2
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288893
Cooper
The glutamine transaminase-ome ...
Canis lupus familiaris, Embryophyta, Enterococcus faecalis, Escherichia coli, Homo sapiens, Lactuca sativa, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Spinacia oleracea
CRC Crit. Rev. Biochem.
4
281-303
1977
-
-
-
-
-
-
-
-
3
-
1
11
-
10
-
-
1
1
-
20
-
-
36
1
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
11
-
-
-
1
-
20
-
-
36
1
-
-
1
-
3
-
1
-
-
-
-
-
-
-
288894
Cooper
The glutamine transaminase-ome ...
Homo sapiens, Rattus norvegicus
J. Neurochem.
28
771-778
1977
-
-
-
-
-
-
-
-
6
-
-
6
-
2
-
-
-
-
-
9
2
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
6
-
-
-
-
-
9
2
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
288886
Fernald
Purification and properties of ...
Bacillus subtilis 168, Bacillus subtilis, Thermus aquaticus, Thermus aquaticus YT-1
Arch. Biochem. Biophys.
153
95-104
1972
2
-
-
-
-
2
6
6
-
2
2
4
-
109
-
-
2
-
-
-
2
2
48
-
4
2
-
-
6
-
-
2
-
-
-
2
-
-
2
-
-
2
-
6
-
6
-
2
2
4
-
-
-
2
-
-
2
2
48
-
4
2
-
-
6
-
-
-
-
-
-
-
-
-
288887
Ramaley
Dicarboxylate omega-amidase of ...
Bacillus subtilis 168, Bacillus subtilis
Arch. Biochem. Biophys.
153
88-94
1972
3
-
-
-
-
-
1
-
2
-
-
-
-
89
-
-
1
-
-
-
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
288888
Hersh
Rat liver omega-amidase. Kinet ...
Rattus norvegicus
Biochemistry
11
2251-2256
1972
2
-
-
-
-
-
6
14
-
-
-
2
4
1
-
-
1
1
-
2
1
-
26
-
1
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
6
-
14
-
-
-
2
4
-
-
1
-
2
1
-
26
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
288892
Hersh
Rat liver omega-amidase. Purof ...
Rattus norvegicus
Biochemistry
10
2884-2891
1971
-
-
-
-
-
-
12
23
1
1
1
4
-
2
-
-
1
1
-
2
2
1
49
1
1
-
2
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
12
-
23
1
1
1
4
-
-
-
1
-
2
2
1
49
1
1
-
2
-
2
-
4
-
-
-
-
-
-
-
288889
Meister
Hydrolysis and transfer reacti ...
Rattus norvegicus
J. Biol. Chem.
215
441-460
1955
1
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
1
1
-
2
-
-
24
-
-
-
-
-
6
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
2
-
-
24
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-