BRENDA - Enzyme Database show
show all sequences of 3.5.1.3

Hydrolysis and transfer reactions catalyzed by omega-amidase preparations

Meister, A.; Levintow, L.; Greenfield, R.E.; Abendschein, P.A.; J. Biol. Chem. 215, 441-460 (1955)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
no activation by ATP, ADP, phosphate or arsenate
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for Mg2+ or Mn2+ for formation of hydroxamate
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoglutaramate + H2O
Rattus norvegicus
alpha-ketoglutaramate
2-oxoglutarate + NH3
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Rattus norvegicus
Reaction
Reaction
Commentary
Organism
a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3
acyl-enzyme mechanism
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutaramate + H2O
best substrate
288889
Rattus norvegicus
2-oxoglutarate + NH3
-
288889
Rattus norvegicus
?
2-oxoglutaramate + H2O
alpha-ketoglutaramate
288889
Rattus norvegicus
2-oxoglutarate + NH3
-
288889
Rattus norvegicus
?
2-oxoglutaramate + hydroxylamine
alpha-ketoglutaramate
288889
Rattus norvegicus
2-oxoglutaryl hydroxamate + NH3
-
-
-
?
2-oxosuccinamate + H2O
alpha-ketosuccinamate
288889
Rattus norvegicus
2-oxosuccinate + NH3
-
-
-
?
2-oxosuccinamate + H2O
no formation of hydroxamate from alpha-ketosuccinamate
288889
Rattus norvegicus
2-oxosuccinate + NH3
-
-
-
?
caproamide + H2O
no hydroxamte formation
288889
Rattus norvegicus
caproic acid + NH3
-
-
-
?
glutaramate + H2O
-
288889
Rattus norvegicus
glutarate + NH3
-
-
-
?
glutaramate + hydroxylamine
amidase activity, hydrolysis proceeds at a slower rate than formation of glutaryl hydroxamate in the presence of hydroxylamine
288889
Rattus norvegicus
glutaryl hydroxamate + NH3
-
-
-
?
glutarate + hydroxylamine
at pH 5, not at pH 7
288889
Rattus norvegicus
glutaryl hydroxamate
-
-
-
?
L-isoasparagine + H2O
poor substrate
288889
Rattus norvegicus
L-aspartate + NH3
-
-
-
?
L-isoglutamine + H2O
poor substrate
288889
Rattus norvegicus
L-glutamate + NH3
-
-
-
?
L-leucine amide + H2O
no hydroxamte formation
288889
Rattus norvegicus
L-Leu + NH3
-
-
-
?
L-phenylalanine amide + H2O
poor substrate, 12% compared with alpha-ketoglutaramate
288889
Rattus norvegicus
L-Phe + NH3
-
-
-
?
additional information
substrate specificity
288889
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes hydrolysis and transfer reactions, enzyme-dicarboxylic acid complex may react either with H2O or hydroxylamine
288889
Rattus norvegicus
?
-
-
-
-
additional information
glutamine, acetamide, propionamide, butyramide, isobutyramide, isovaleramide, oxamate, malonamate, adipamate, oxamide, adipamide, alpha-ketoadipamate, alpha-keto-DL-gamma-methylglutaramate, DL-alpha-aminomalonamate, alpha-methyl-DL-asparagine, DL-gamma-methyl-L-glutamine, alpha-methyl-DL-glutamine, DL-beta-methyl-DL-glutamine, DL-proline, DL-alanine and L-tyrosine amide: no substrates
288889
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes hydroxaminolysis and transamidation reactions with esters and amides
288889
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes hydrolysis and hydroxaminolysis
288889
Rattus norvegicus
?
-
-
-
-
succinamate + H2O
amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine
288889
Rattus norvegicus
succinic acid + NH3
-
-
-
?
succinamate + hydroxylamine
catalyzes formation of hydroxamate much more rapidly than hydrolysis of the amide
288889
Rattus norvegicus
succinyl hydroxamate + NH3
-
-
-
r
succinate + hydroxylamine
at pH 5, not at pH 7
288889
Rattus norvegicus
succinyl hydroxamate + H2O
-
-
-
r
succinyl hydroxamate + H2O
-
288889
Rattus norvegicus
succinate + hydroxylamine
-
-
-
r
succinylmonohydroxamate + H2O
-
288889
Rattus norvegicus
succinate + hydroxylamine
-
-
-
r
valeramide + H2O
poor substrate
288889
Rattus norvegicus
valeric acid + NH3
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Rattus norvegicus
5
-
hydroxamate formation, substrate glutarate or succinate
Rattus norvegicus
6.5
7.5
broad pH-optimum, substrate glutaramate or succinamate; hydroxaminolysis
Rattus norvegicus
7
-
hydrolysis and transfer reactions; substrate: succinamate or glutaramate, assay at
Rattus norvegicus
8.5
9
pH-optimum for hydroxaminolysis of alpha-ketoglutaramate
Rattus norvegicus
9
-
optimal for hydrolysis of alpha-ketoglutaramate or alpha-ketosuccinamate
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no requirement for any cofactors for hydroxamate formation
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
no activation by ATP, ADP, phosphate or arsenate
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no requirement for any cofactors for hydroxamate formation
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Rattus norvegicus
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for Mg2+ or Mn2+ for formation of hydroxamate
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoglutaramate + H2O
Rattus norvegicus
alpha-ketoglutaramate
2-oxoglutarate + NH3
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutaramate + H2O
best substrate
288889
Rattus norvegicus
2-oxoglutarate + NH3
-
288889
Rattus norvegicus
?
2-oxoglutaramate + H2O
alpha-ketoglutaramate
288889
Rattus norvegicus
2-oxoglutarate + NH3
-
288889
Rattus norvegicus
?
2-oxoglutaramate + hydroxylamine
alpha-ketoglutaramate
288889
Rattus norvegicus
2-oxoglutaryl hydroxamate + NH3
-
-
-
?
2-oxosuccinamate + H2O
alpha-ketosuccinamate
288889
Rattus norvegicus
2-oxosuccinate + NH3
-
-
-
?
2-oxosuccinamate + H2O
no formation of hydroxamate from alpha-ketosuccinamate
288889
Rattus norvegicus
2-oxosuccinate + NH3
-
-
-
?
caproamide + H2O
no hydroxamte formation
288889
Rattus norvegicus
caproic acid + NH3
-
-
-
?
glutaramate + H2O
-
288889
Rattus norvegicus
glutarate + NH3
-
-
-
?
glutaramate + hydroxylamine
amidase activity, hydrolysis proceeds at a slower rate than formation of glutaryl hydroxamate in the presence of hydroxylamine
288889
Rattus norvegicus
glutaryl hydroxamate + NH3
-
-
-
?
glutarate + hydroxylamine
at pH 5, not at pH 7
288889
Rattus norvegicus
glutaryl hydroxamate
-
-
-
?
L-isoasparagine + H2O
poor substrate
288889
Rattus norvegicus
L-aspartate + NH3
-
-
-
?
L-isoglutamine + H2O
poor substrate
288889
Rattus norvegicus
L-glutamate + NH3
-
-
-
?
L-leucine amide + H2O
no hydroxamte formation
288889
Rattus norvegicus
L-Leu + NH3
-
-
-
?
L-phenylalanine amide + H2O
poor substrate, 12% compared with alpha-ketoglutaramate
288889
Rattus norvegicus
L-Phe + NH3
-
-
-
?
additional information
substrate specificity
288889
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes hydrolysis and transfer reactions, enzyme-dicarboxylic acid complex may react either with H2O or hydroxylamine
288889
Rattus norvegicus
?
-
-
-
-
additional information
glutamine, acetamide, propionamide, butyramide, isobutyramide, isovaleramide, oxamate, malonamate, adipamate, oxamide, adipamide, alpha-ketoadipamate, alpha-keto-DL-gamma-methylglutaramate, DL-alpha-aminomalonamate, alpha-methyl-DL-asparagine, DL-gamma-methyl-L-glutamine, alpha-methyl-DL-glutamine, DL-beta-methyl-DL-glutamine, DL-proline, DL-alanine and L-tyrosine amide: no substrates
288889
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes hydroxaminolysis and transamidation reactions with esters and amides
288889
Rattus norvegicus
?
-
-
-
-
additional information
catalyzes hydrolysis and hydroxaminolysis
288889
Rattus norvegicus
?
-
-
-
-
succinamate + H2O
amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine
288889
Rattus norvegicus
succinic acid + NH3
-
-
-
?
succinamate + hydroxylamine
catalyzes formation of hydroxamate much more rapidly than hydrolysis of the amide
288889
Rattus norvegicus
succinyl hydroxamate + NH3
-
-
-
r
succinate + hydroxylamine
at pH 5, not at pH 7
288889
Rattus norvegicus
succinyl hydroxamate + H2O
-
-
-
r
succinyl hydroxamate + H2O
-
288889
Rattus norvegicus
succinate + hydroxylamine
-
-
-
r
succinylmonohydroxamate + H2O
-
288889
Rattus norvegicus
succinate + hydroxylamine
-
-
-
r
valeramide + H2O
poor substrate
288889
Rattus norvegicus
valeric acid + NH3
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Rattus norvegicus
5
-
hydroxamate formation, substrate glutarate or succinate
Rattus norvegicus
6.5
7.5
broad pH-optimum, substrate glutaramate or succinamate; hydroxaminolysis
Rattus norvegicus
7
-
hydrolysis and transfer reactions; substrate: succinamate or glutaramate, assay at
Rattus norvegicus
8.5
9
pH-optimum for hydroxaminolysis of alpha-ketoglutaramate
Rattus norvegicus
9
-
optimal for hydrolysis of alpha-ketoglutaramate or alpha-ketosuccinamate
Rattus norvegicus
Other publictions for EC 3.5.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734871
Zhang
Identification and characteriz ...
Arabidopsis thaliana, Arabidopsis thaliana Col
Phytochemistry
99
36-43
2014
-
-
1
-
-
-
-
3
2
-
2
2
-
5
-
-
1
-
-
1
-
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
2
-
2
2
-
-
-
1
-
1
-
-
10
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
721018
Cobzaru
Homologous gene clusters of ni ...
Nocardioides sp., Nocardioides sp. JS614 / ATCC BAA-499, Paenarthrobacter nicotinovorans, Rhodococcus opacus
Res. Microbiol.
162
285-291
2011
-
-
2
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
5
-
3
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
5
-
3
-
-
-
3
-
-
-
-
6
6
-
-
-
710840
Krasnikov
Assay and purification of omeg ...
Rattus norvegicus
Anal. Biochem.
391
144-150
2009
-
1
-
-
-
-
-
-
4
-
1
-
-
4
-
-
1
-
-
4
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
1
-
4
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
711316
Jaisson
Molecular identification of om ...
Mus musculus
Biochimie
91
1066-1071
2009
-
-
1
-
-
-
-
8
-
-
-
-
-
5
-
-
1
-
-
1
-
-
6
-
1
-
-
8
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
1
-
1
-
-
6
-
1
-
-
8
2
-
-
-
-
-
-
-
8
8
711317
Krasnikov
Identification of the putative ...
Homo sapiens, Rattus norvegicus
Biochimie
91
1072-1080
2009
-
-
1
-
-
-
-
3
-
-
8
4
-
4
-
-
2
-
-
3
-
-
4
2
2
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
8
4
-
-
-
2
-
3
-
-
4
2
2
-
-
-
2
-
-
-
-
1
1
-
-
-
288890
Makar
Glutamine transaminase K and o ...
Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus
J. Neurochem.
62
1983-1988
1994
-
-
-
-
-
-
-
-
-
-
-
8
-
8
-
-
-
-
-
20
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
20
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288891
Cooper
High activities of glutamine t ...
Rattus norvegicus
J. Neurochem.
61
1731-1741
1993
-
-
-
-
-
-
-
-
3
-
-
5
-
4
-
-
-
-
-
7
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
5
-
-
-
-
-
7
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288884
Cooper
alpha-Keto acid omega-amidase ...
Embryophyta, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae
Methods Enzymol.
113
350-358
1985
-
3
-
-
-
2
8
3
5
1
2
8
-
6
1
-
1
1
1
13
2
2
24
1
-
-
3
-
2
-
-
-
-
-
-
-
3
-
-
-
-
2
-
8
-
3
5
1
2
8
-
1
-
1
1
13
2
2
24
1
-
-
3
-
2
-
-
-
-
-
-
-
-
-
288885
Calderon
omega-Amidase pathway in the d ...
Neurospora crassa
J. Bacteriol.
161
807-809
1985
-
-
-
-
-
-
3
-
-
-
-
2
-
2
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288893
Cooper
The glutamine transaminase-ome ...
Canis lupus familiaris, Embryophyta, Enterococcus faecalis, Escherichia coli, Homo sapiens, Lactuca sativa, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Spinacia oleracea
CRC Crit. Rev. Biochem.
4
281-303
1977
-
-
-
-
-
-
-
-
3
-
1
11
-
10
-
-
1
1
-
20
-
-
36
1
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
11
-
-
-
1
-
20
-
-
36
1
-
-
1
-
3
-
1
-
-
-
-
-
-
-
288894
Cooper
The glutamine transaminase-ome ...
Homo sapiens, Rattus norvegicus
J. Neurochem.
28
771-778
1977
-
-
-
-
-
-
-
-
6
-
-
6
-
2
-
-
-
-
-
9
2
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
6
-
-
-
-
-
9
2
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
288886
Fernald
Purification and properties of ...
Bacillus subtilis 168, Bacillus subtilis, Thermus aquaticus, Thermus aquaticus YT-1
Arch. Biochem. Biophys.
153
95-104
1972
2
-
-
-
-
2
6
6
-
2
2
4
-
109
-
-
2
-
-
-
2
2
48
-
4
2
-
-
6
-
-
2
-
-
-
2
-
-
2
-
-
2
-
6
-
6
-
2
2
4
-
-
-
2
-
-
2
2
48
-
4
2
-
-
6
-
-
-
-
-
-
-
-
-
288887
Ramaley
Dicarboxylate omega-amidase of ...
Bacillus subtilis 168, Bacillus subtilis
Arch. Biochem. Biophys.
153
88-94
1972
3
-
-
-
-
-
1
-
2
-
-
-
-
89
-
-
1
-
-
-
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
288888
Hersh
Rat liver omega-amidase. Kinet ...
Rattus norvegicus
Biochemistry
11
2251-2256
1972
2
-
-
-
-
-
6
14
-
-
-
2
4
1
-
-
1
1
-
2
1
-
26
-
1
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
6
-
14
-
-
-
2
4
-
-
1
-
2
1
-
26
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
288892
Hersh
Rat liver omega-amidase. Purof ...
Rattus norvegicus
Biochemistry
10
2884-2891
1971
-
-
-
-
-
-
12
23
1
1
1
4
-
2
-
-
1
1
-
2
2
1
49
1
1
-
2
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
12
-
23
1
1
1
4
-
-
-
1
-
2
2
1
49
1
1
-
2
-
2
-
4
-
-
-
-
-
-
-
288889
Meister
Hydrolysis and transfer reacti ...
Rattus norvegicus
J. Biol. Chem.
215
441-460
1955
1
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
1
1
-
2
-
-
24
-
-
-
-
-
6
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
2
-
-
24
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-