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show all sequences of 3.5.1.3

Purification and properties of dicarboxylate omega-amidase from Bacillus subtilis 168 and Thermus aquaticus YT-1

Fernald, N.J.; Ramaley, R.F.; Arch. Biochem. Biophys. 153, 95-104 (1972)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
no activation by ATP or Coenzyme A
Bacillus subtilis
additional information
no activation by ATP or Coenzyme A
Thermus aquaticus
General Stability
General Stability
Organism
does not require mercaptoethanol or succinate for stability
Thermus aquaticus
does not require mercaptoethanol or succinate for stability
Bacillus subtilis
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
amide and acyl transfer activity: no inhibition by high concentrations of hydroxylamine; no inhibition by EDTA
Bacillus subtilis
additional information
amide and acyl transfer activity: no inhibition by high concentrations of hydroxylamine; no inhibition by EDTA
Thermus aquaticus
NH4+
biosynthesis of omega-amidase is repressed by free ammonium ions
Bacillus subtilis
NH4+
synthesis of omega-amidase is repressed by about 50%
Thermus aquaticus
phosphate
phosphate buffer: slightly inhibitory
Bacillus subtilis
phosphate
phosphate buffer: slightly inhibitory
Thermus aquaticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3
-
succinamate
-
Bacillus subtilis
1.34
-
succinamate
-
Thermus aquaticus
30
-
succinate
-
Thermus aquaticus
50
-
hydroxylamine
with substrate succinamate
Bacillus subtilis
63
-
succinate
-
Bacillus subtilis
100
-
hydroxylamine
with substrate succinamate
Thermus aquaticus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no activation by Mg2+; no Mg2+ requirement for omega-amidase activity; no requirement for any metal ions for hydroxamate formation
Bacillus subtilis
additional information
no activation by Mg2+; no Mg2+ requirement for omega-amidase activity; no requirement for any metal ions for hydroxamate formation
Thermus aquaticus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
38500
gel filtration
Thermus aquaticus
120000
-
gel filtration
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoglutaramate + H2O
Thermus aquaticus
alpha-ketoglutaramate
2-oxoglutarate + NH3
-
Thermus aquaticus
?
2-oxoglutaramate + H2O
Bacillus subtilis
alpha-ketoglutaramate
2-oxoglutarate + NH3
-
Bacillus subtilis
?
2-oxoglutaramate + H2O
Thermus aquaticus
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
2-oxoglutarate + NH3
-
Thermus aquaticus
?
2-oxoglutaramate + H2O
Bacillus subtilis
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
2-oxoglutarate + NH3
-
Bacillus subtilis
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
168, wild-type
-
Bacillus subtilis 168
-
168, wild-type
-
Thermus aquaticus
-
gram-negative extreme thermophilic bacterium; YT-1
-
Thermus aquaticus YT-1
-
YT-1
-
Purification (Commentary)
Commentary
Organism
-
Bacillus subtilis
-
Thermus aquaticus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.9
-
-
Bacillus subtilis
66
-
-
Thermus aquaticus
Storage Stability
Storage Stability
Organism
-20°C, protein concentration above 0.1 mg/ml, 0.05 M potassium phosphate, pH 7.2, 0.05 M KCl, 3 months, quite stable
Bacillus subtilis
-20°C, protein concentration above 0.1 mg/ml, 3 months, quite stable
Thermus aquaticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutaramate + H2O
alpha-ketoglutaramate
288886
Thermus aquaticus
2-oxoglutarate + NH3
-
288886
Thermus aquaticus
?
2-oxoglutaramate + H2O
alpha-ketoglutaramate
288886
Bacillus subtilis
2-oxoglutarate + NH3
-
288886
Bacillus subtilis
?
2-oxoglutaramate + H2O
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
288886
Thermus aquaticus
2-oxoglutarate + NH3
-
288886
Thermus aquaticus
?
2-oxoglutaramate + H2O
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
288886
Bacillus subtilis
2-oxoglutarate + NH3
-
288886
Bacillus subtilis
?
asparagine + H2O
-
288886
Bacillus subtilis
aspartate + NH3
-
-
-
?
fumarate + H2O
-
288886
Thermus aquaticus
?
-
-
-
?
fumarate + hydroxylamine
acyl transfer reaction
288886
Thermus aquaticus
fumaryl hydroxamate + H2O
-
-
-
?
L-asparagine + hydroxylamine
amide transfer reaction
288886
Bacillus subtilis
L-asparaginyl hydroxamate + H2O
-
-
-
?
malate + H2O
-
288886
Thermus aquaticus
?
-
-
-
?
malate + H2O
-
288886
Bacillus subtilis
?
-
-
-
?
malate + hydroxylamine
acyl transfer reaction
288886
Thermus aquaticus
malyl hydroxamate + H2O
-
-
-
?
malate + hydroxylamine
acyl transfer reaction
288886
Bacillus subtilis
malyl hydroxamate + H2O
-
-
-
?
additional information
substrate specificity
288886
Thermus aquaticus
?
-
-
-
-
additional information
substrate specificity
288886
Bacillus subtilis
?
-
-
-
-
additional information
no reaction with monocarboxylic acids
288886
Thermus aquaticus
?
-
-
-
-
additional information
no reaction with monocarboxylic acids
288886
Bacillus subtilis
?
-
-
-
-
additional information
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
288886
Thermus aquaticus
?
-
-
-
-
additional information
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
288886
Bacillus subtilis
?
-
-
-
-
additional information
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
288886
Thermus aquaticus
?
-
-
-
-
additional information
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
288886
Bacillus subtilis
?
-
-
-
-
additional information
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
288886
Thermus aquaticus
?
-
-
-
-
additional information
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
288886
Bacillus subtilis
?
-
-
-
-
additional information
substrate specificity
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
no reaction with monocarboxylic acids
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
substrate specificity
288886
Thermus aquaticus YT-1
?
-
-
-
-
additional information
no reaction with monocarboxylic acids
288886
Thermus aquaticus YT-1
?
-
-
-
-
additional information
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
288886
Thermus aquaticus YT-1
?
-
-
-
-
additional information
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
288886
Thermus aquaticus YT-1
?
-
-
-
-
additional information
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
288886
Thermus aquaticus YT-1
?
-
-
-
-
succinamate + H2O
amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine
288886
Thermus aquaticus
succinic acid + NH3
-
-
-
?
succinamate + H2O
amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine
288886
Bacillus subtilis
succinic acid + NH3
-
-
-
?
succinamate + hydroxylamine
amide transfer reaction
288886
Thermus aquaticus
succinyl hydroxamate + NH3
-
-
-
r
succinamate + hydroxylamine
amide transfer reaction
288886
Bacillus subtilis
succinyl hydroxamate + NH3
-
-
-
r
succinamide + H2O
-
288886
Thermus aquaticus
succinate + NH3
-
-
-
?
succinamide + H2O
-
288886
Bacillus subtilis
succinate + NH3
-
-
-
?
succinamide + hydroxylamine
amide transfer reaction
288886
Thermus aquaticus
succinyl hydroxamate + ?
-
-
-
r
succinamide + hydroxylamine
amide transfer reaction
288886
Bacillus subtilis
succinyl hydroxamate + ?
-
-
-
r
succinate + hydroxylamine
very high substrate concentration required for acyl transfer reaction
288886
Thermus aquaticus
succinyl hydroxamate + H2O
-
-
-
r
succinate + hydroxylamine
very high substrate concentration required for acyl transfer reaction
288886
Bacillus subtilis
succinyl hydroxamate + H2O
-
-
-
r
succinate + hydroxylamine
acyl transfer reaction
288886
Thermus aquaticus
succinyl hydroxamate + H2O
-
-
-
r
succinate + hydroxylamine
acyl transfer reaction
288886
Bacillus subtilis
succinyl hydroxamate + H2O
-
-
-
r
succinimide + hydroxylamine
amide transfer reaction
288886
Thermus aquaticus
?
-
-
-
?
succinimide + hydroxylamine
amide transfer reaction
288886
Bacillus subtilis
?
-
-
-
?
succinyl hydroxamate + H2O
-
288886
Thermus aquaticus
succinate + hydroxylamine
-
-
-
r
succinyl hydroxamate + H2O
-
288886
Bacillus subtilis
succinate + hydroxylamine
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
45
56
broad temperature optimum
Bacillus subtilis
70
-
assay at
Thermus aquaticus
80
-
-
Thermus aquaticus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
63
about 50% of maximum activity
Bacillus subtilis
63
87
about 50% of maximum activity
Thermus aquaticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Bacillus subtilis
additional information
-
-
Thermus aquaticus
6.5
-
acyl transferase activity, sharp optimum
Bacillus subtilis
6.5
-
acyl transferase activity, sharp optimum
Thermus aquaticus
7
9
amide transferase activity, broad optimum
Bacillus subtilis
7
9
amide transferase activity, broad optimum
Thermus aquaticus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no requirement for any cofactors for hydroxamate formation
Bacillus subtilis
additional information
no requirement for any cofactors for hydroxamate formation
Thermus aquaticus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
no activation by ATP or Coenzyme A
Bacillus subtilis
additional information
no activation by ATP or Coenzyme A
Thermus aquaticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no requirement for any cofactors for hydroxamate formation
Bacillus subtilis
additional information
no requirement for any cofactors for hydroxamate formation
Thermus aquaticus
General Stability (protein specific)
General Stability
Organism
does not require mercaptoethanol or succinate for stability
Thermus aquaticus
does not require mercaptoethanol or succinate for stability
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
amide and acyl transfer activity: no inhibition by high concentrations of hydroxylamine; no inhibition by EDTA
Bacillus subtilis
additional information
amide and acyl transfer activity: no inhibition by high concentrations of hydroxylamine; no inhibition by EDTA
Thermus aquaticus
NH4+
biosynthesis of omega-amidase is repressed by free ammonium ions
Bacillus subtilis
NH4+
synthesis of omega-amidase is repressed by about 50%
Thermus aquaticus
phosphate
phosphate buffer: slightly inhibitory
Bacillus subtilis
phosphate
phosphate buffer: slightly inhibitory
Thermus aquaticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3
-
succinamate
-
Bacillus subtilis
1.34
-
succinamate
-
Thermus aquaticus
30
-
succinate
-
Thermus aquaticus
50
-
hydroxylamine
with substrate succinamate
Bacillus subtilis
63
-
succinate
-
Bacillus subtilis
100
-
hydroxylamine
with substrate succinamate
Thermus aquaticus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no activation by Mg2+; no Mg2+ requirement for omega-amidase activity; no requirement for any metal ions for hydroxamate formation
Bacillus subtilis
additional information
no activation by Mg2+; no Mg2+ requirement for omega-amidase activity; no requirement for any metal ions for hydroxamate formation
Thermus aquaticus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
38500
gel filtration
Thermus aquaticus
120000
-
gel filtration
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoglutaramate + H2O
Thermus aquaticus
alpha-ketoglutaramate
2-oxoglutarate + NH3
-
Thermus aquaticus
?
2-oxoglutaramate + H2O
Bacillus subtilis
alpha-ketoglutaramate
2-oxoglutarate + NH3
-
Bacillus subtilis
?
2-oxoglutaramate + H2O
Thermus aquaticus
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
2-oxoglutarate + NH3
-
Thermus aquaticus
?
2-oxoglutaramate + H2O
Bacillus subtilis
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
2-oxoglutarate + NH3
-
Bacillus subtilis
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus subtilis
-
Thermus aquaticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.9
-
-
Bacillus subtilis
66
-
-
Thermus aquaticus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, protein concentration above 0.1 mg/ml, 0.05 M potassium phosphate, pH 7.2, 0.05 M KCl, 3 months, quite stable
Bacillus subtilis
-20°C, protein concentration above 0.1 mg/ml, 3 months, quite stable
Thermus aquaticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutaramate + H2O
alpha-ketoglutaramate
288886
Thermus aquaticus
2-oxoglutarate + NH3
-
288886
Thermus aquaticus
?
2-oxoglutaramate + H2O
alpha-ketoglutaramate
288886
Bacillus subtilis
2-oxoglutarate + NH3
-
288886
Bacillus subtilis
?
2-oxoglutaramate + H2O
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
288886
Thermus aquaticus
2-oxoglutarate + NH3
-
288886
Thermus aquaticus
?
2-oxoglutaramate + H2O
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
288886
Bacillus subtilis
2-oxoglutarate + NH3
-
288886
Bacillus subtilis
?
asparagine + H2O
-
288886
Bacillus subtilis
aspartate + NH3
-
-
-
?
fumarate + H2O
-
288886
Thermus aquaticus
?
-
-
-
?
fumarate + hydroxylamine
acyl transfer reaction
288886
Thermus aquaticus
fumaryl hydroxamate + H2O
-
-
-
?
L-asparagine + hydroxylamine
amide transfer reaction
288886
Bacillus subtilis
L-asparaginyl hydroxamate + H2O
-
-
-
?
malate + H2O
-
288886
Thermus aquaticus
?
-
-
-
?
malate + H2O
-
288886
Bacillus subtilis
?
-
-
-
?
malate + hydroxylamine
acyl transfer reaction
288886
Thermus aquaticus
malyl hydroxamate + H2O
-
-
-
?
malate + hydroxylamine
acyl transfer reaction
288886
Bacillus subtilis
malyl hydroxamate + H2O
-
-
-
?
additional information
substrate specificity
288886
Thermus aquaticus
?
-
-
-
-
additional information
substrate specificity
288886
Bacillus subtilis
?
-
-
-
-
additional information
no reaction with monocarboxylic acids
288886
Thermus aquaticus
?
-
-
-
-
additional information
no reaction with monocarboxylic acids
288886
Bacillus subtilis
?
-
-
-
-
additional information
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
288886
Thermus aquaticus
?
-
-
-
-
additional information
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
288886
Bacillus subtilis
?
-
-
-
-
additional information
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
288886
Thermus aquaticus
?
-
-
-
-
additional information
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
288886
Bacillus subtilis
?
-
-
-
-
additional information
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
288886
Thermus aquaticus
?
-
-
-
-
additional information
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
288886
Bacillus subtilis
?
-
-
-
-
additional information
substrate specificity
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
no reaction with monocarboxylic acids
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
288886
Bacillus subtilis 168
?
-
-
-
-
additional information
substrate specificity
288886
Thermus aquaticus YT-1
?
-
-
-
-
additional information
no reaction with monocarboxylic acids
288886
Thermus aquaticus YT-1
?
-
-
-
-
additional information
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
288886
Thermus aquaticus YT-1
?
-
-
-
-
additional information
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
288886
Thermus aquaticus YT-1
?
-
-
-
-
additional information
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
288886
Thermus aquaticus YT-1
?
-
-
-
-
succinamate + H2O
amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine
288886
Thermus aquaticus
succinic acid + NH3
-
-
-
?
succinamate + H2O
amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine
288886
Bacillus subtilis
succinic acid + NH3
-
-
-
?
succinamate + hydroxylamine
amide transfer reaction
288886
Thermus aquaticus
succinyl hydroxamate + NH3
-
-
-
r
succinamate + hydroxylamine
amide transfer reaction
288886
Bacillus subtilis
succinyl hydroxamate + NH3
-
-
-
r
succinamide + H2O
-
288886
Thermus aquaticus
succinate + NH3
-
-
-
?
succinamide + H2O
-
288886
Bacillus subtilis
succinate + NH3
-
-
-
?
succinamide + hydroxylamine
amide transfer reaction
288886
Thermus aquaticus
succinyl hydroxamate + ?
-
-
-
r
succinamide + hydroxylamine
amide transfer reaction
288886
Bacillus subtilis
succinyl hydroxamate + ?
-
-
-
r
succinate + hydroxylamine
very high substrate concentration required for acyl transfer reaction
288886
Thermus aquaticus
succinyl hydroxamate + H2O
-
-
-
r
succinate + hydroxylamine
very high substrate concentration required for acyl transfer reaction
288886
Bacillus subtilis
succinyl hydroxamate + H2O
-
-
-
r
succinate + hydroxylamine
acyl transfer reaction
288886
Thermus aquaticus
succinyl hydroxamate + H2O
-
-
-
r
succinate + hydroxylamine
acyl transfer reaction
288886
Bacillus subtilis
succinyl hydroxamate + H2O
-
-
-
r
succinimide + hydroxylamine
amide transfer reaction
288886
Thermus aquaticus
?
-
-
-
?
succinimide + hydroxylamine
amide transfer reaction
288886
Bacillus subtilis
?
-
-
-
?
succinyl hydroxamate + H2O
-
288886
Thermus aquaticus
succinate + hydroxylamine
-
-
-
r
succinyl hydroxamate + H2O
-
288886
Bacillus subtilis
succinate + hydroxylamine
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
45
56
broad temperature optimum
Bacillus subtilis
70
-
assay at
Thermus aquaticus
80
-
-
Thermus aquaticus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
63
about 50% of maximum activity
Bacillus subtilis
63
87
about 50% of maximum activity
Thermus aquaticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
-
Bacillus subtilis
additional information
-
-
Thermus aquaticus
6.5
-
acyl transferase activity, sharp optimum
Bacillus subtilis
6.5
-
acyl transferase activity, sharp optimum
Thermus aquaticus
7
9
amide transferase activity, broad optimum
Bacillus subtilis
7
9
amide transferase activity, broad optimum
Thermus aquaticus
Other publictions for EC 3.5.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734871
Zhang
Identification and characteriz ...
Arabidopsis thaliana, Arabidopsis thaliana Col
Phytochemistry
99
36-43
2014
-
-
1
-
-
-
-
3
2
-
2
2
-
5
-
-
1
-
-
1
-
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
2
-
2
2
-
-
-
1
-
1
-
-
10
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
721018
Cobzaru
Homologous gene clusters of ni ...
Nocardioides sp., Nocardioides sp. JS614 / ATCC BAA-499, Paenarthrobacter nicotinovorans, Rhodococcus opacus
Res. Microbiol.
162
285-291
2011
-
-
2
-
-
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
5
-
3
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
5
-
3
-
-
-
3
-
-
-
-
6
6
-
-
-
710840
Krasnikov
Assay and purification of omeg ...
Rattus norvegicus
Anal. Biochem.
391
144-150
2009
-
1
-
-
-
-
-
-
4
-
1
-
-
4
-
-
1
-
-
4
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
1
-
4
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
711316
Jaisson
Molecular identification of om ...
Mus musculus
Biochimie
91
1066-1071
2009
-
-
1
-
-
-
-
8
-
-
-
-
-
5
-
-
1
-
-
1
-
-
6
-
1
-
-
8
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
1
-
1
-
-
6
-
1
-
-
8
2
-
-
-
-
-
-
-
8
8
711317
Krasnikov
Identification of the putative ...
Homo sapiens, Rattus norvegicus
Biochimie
91
1072-1080
2009
-
-
1
-
-
-
-
3
-
-
8
4
-
4
-
-
2
-
-
3
-
-
4
2
2
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
8
4
-
-
-
2
-
3
-
-
4
2
2
-
-
-
2
-
-
-
-
1
1
-
-
-
288890
Makar
Glutamine transaminase K and o ...
Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus
J. Neurochem.
62
1983-1988
1994
-
-
-
-
-
-
-
-
-
-
-
8
-
8
-
-
-
-
-
20
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
20
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288891
Cooper
High activities of glutamine t ...
Rattus norvegicus
J. Neurochem.
61
1731-1741
1993
-
-
-
-
-
-
-
-
3
-
-
5
-
4
-
-
-
-
-
7
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
5
-
-
-
-
-
7
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288884
Cooper
alpha-Keto acid omega-amidase ...
Embryophyta, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae
Methods Enzymol.
113
350-358
1985
-
3
-
-
-
2
8
3
5
1
2
8
-
6
1
-
1
1
1
13
2
2
24
1
-
-
3
-
2
-
-
-
-
-
-
-
3
-
-
-
-
2
-
8
-
3
5
1
2
8
-
1
-
1
1
13
2
2
24
1
-
-
3
-
2
-
-
-
-
-
-
-
-
-
288885
Calderon
omega-Amidase pathway in the d ...
Neurospora crassa
J. Bacteriol.
161
807-809
1985
-
-
-
-
-
-
3
-
-
-
-
2
-
2
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288893
Cooper
The glutamine transaminase-ome ...
Canis lupus familiaris, Embryophyta, Enterococcus faecalis, Escherichia coli, Homo sapiens, Lactuca sativa, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Spinacia oleracea
CRC Crit. Rev. Biochem.
4
281-303
1977
-
-
-
-
-
-
-
-
3
-
1
11
-
10
-
-
1
1
-
20
-
-
36
1
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
11
-
-
-
1
-
20
-
-
36
1
-
-
1
-
3
-
1
-
-
-
-
-
-
-
288894
Cooper
The glutamine transaminase-ome ...
Homo sapiens, Rattus norvegicus
J. Neurochem.
28
771-778
1977
-
-
-
-
-
-
-
-
6
-
-
6
-
2
-
-
-
-
-
9
2
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
6
-
-
-
-
-
9
2
-
6
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
288886
Fernald
Purification and properties of ...
Bacillus subtilis 168, Bacillus subtilis, Thermus aquaticus, Thermus aquaticus YT-1
Arch. Biochem. Biophys.
153
95-104
1972
2
-
-
-
-
2
6
6
-
2
2
4
-
109
-
-
2
-
-
-
2
2
48
-
4
2
-
-
6
-
-
2
-
-
-
2
-
-
2
-
-
2
-
6
-
6
-
2
2
4
-
-
-
2
-
-
2
2
48
-
4
2
-
-
6
-
-
-
-
-
-
-
-
-
288887
Ramaley
Dicarboxylate omega-amidase of ...
Bacillus subtilis 168, Bacillus subtilis
Arch. Biochem. Biophys.
153
88-94
1972
3
-
-
-
-
-
1
-
2
-
-
-
-
89
-
-
1
-
-
-
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
288888
Hersh
Rat liver omega-amidase. Kinet ...
Rattus norvegicus
Biochemistry
11
2251-2256
1972
2
-
-
-
-
-
6
14
-
-
-
2
4
1
-
-
1
1
-
2
1
-
26
-
1
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
6
-
14
-
-
-
2
4
-
-
1
-
2
1
-
26
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
288892
Hersh
Rat liver omega-amidase. Purof ...
Rattus norvegicus
Biochemistry
10
2884-2891
1971
-
-
-
-
-
-
12
23
1
1
1
4
-
2
-
-
1
1
-
2
2
1
49
1
1
-
2
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
12
-
23
1
1
1
4
-
-
-
1
-
2
2
1
49
1
1
-
2
-
2
-
4
-
-
-
-
-
-
-
288889
Meister
Hydrolysis and transfer reacti ...
Rattus norvegicus
J. Biol. Chem.
215
441-460
1955
1
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
1
1
-
2
-
-
24
-
-
-
-
-
6
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
2
-
-
24
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-