BRENDA - Enzyme Database
show all sequences of 3.5.1.29

Enzymes of vitamin B6 degradation. Purification and properties of two N-acetylamidohydrolases

Huynh, M.S.; Snell, E.E.; J. Biol. Chem. 260, 2379-2383 (1985)

Data extracted from this reference:

General Stability
General Stability
Organism
stable at room temperature between pH 6 and 10
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-hydroxyglutarate
competitive inhibition
Pseudomonas sp.
alpha-ketoglutarate
-
Arthrobacter sp.
alpha-ketoglutarate
competitive inhibition
Pseudomonas sp.
citrate
competitive inhibition
Pseudomonas sp.
diphosphate
-
Arthrobacter sp.
diphosphate
competitive inhibition
Pseudomonas sp.
fumarate
-
Arthrobacter sp.
fumarate
competitive inhibition
Pseudomonas sp.
glutamate
competitive inhibition
Pseudomonas sp.
Glutarate
competitive inhibition
Pseudomonas sp.
Hg2+
-
Pseudomonas sp.
Itaconate
competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit
Arthrobacter sp.
Itaconate
competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit
Pseudomonas sp.
malate
competitive inhibition
Pseudomonas sp.
Maleate
competitive inhibition
Pseudomonas sp.
malonate
competitive inhibition
Pseudomonas sp.
N-acetyl-DL-aspartate
-
Arthrobacter sp.
N-acetyl-DL-aspartate
competitive inhibition
Pseudomonas sp.
oxaloacetate
-
Arthrobacter sp.
oxaloacetate
competitive inhibition
Pseudomonas sp.
phosphate
-
Arthrobacter sp.
phosphate
competitive inhibition
Pseudomonas sp.
SO32-
inhibitory effect increases rapidly with time to a constant level, not competitve with substrate
Arthrobacter sp.
SO32-
inhibitory effect increases rapidly with time to a constant level, not competitve with substrate
Pseudomonas sp.
succinate
-
Arthrobacter sp.
succinate
competitive inhibition
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
(N-acetylaminomethylene)succinic acid
at pH 6.5 and 25°C
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32500
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-(acetamidomethylene)succinate + 2 H2O
Pseudomonas sp.
-
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
2-(acetamidomethylene)succinate + 2 H2O
Pseudomonas sp. IA
-
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arthrobacter sp.
-
Cr-7
-
Pseudomonas sp.
-
MA-1
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.12
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp.
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
-
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp.
acetate + succinate semialdehyde + NH3 + CO2
same products as formed by acid hydrolysis
172028
Pseudomonas sp.
ir
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp. IA
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
-
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp. IA
acetate + succinate semialdehyde + NH3 + CO2
same products as formed by acid hydrolysis
172028
Pseudomonas sp. IA
ir
Subunits
Subunits
Commentary
Organism
monomer
-
Arthrobacter sp.
monomer
SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
90
at pH 8 and 70°C stable for 1 min, loses activity completely in 1 min at 90°C
Pseudomonas sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.67
-
(N-acetylaminomethylene)succinic acid
-
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
pH 6.5 in 50 mM sodium phosphate, MES and HEPES buffers, pH 7.5 in Tris buffer
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
most efficient between pH 6.0 and 7.0
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
loses activity rapidly between 4.0 and 5.0
Pseudomonas sp.
General Stability (protein specific)
General Stability
Organism
stable at room temperature between pH 6 and 10
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-hydroxyglutarate
competitive inhibition
Pseudomonas sp.
alpha-ketoglutarate
-
Arthrobacter sp.
alpha-ketoglutarate
competitive inhibition
Pseudomonas sp.
citrate
competitive inhibition
Pseudomonas sp.
diphosphate
-
Arthrobacter sp.
diphosphate
competitive inhibition
Pseudomonas sp.
fumarate
-
Arthrobacter sp.
fumarate
competitive inhibition
Pseudomonas sp.
glutamate
competitive inhibition
Pseudomonas sp.
Glutarate
competitive inhibition
Pseudomonas sp.
Hg2+
-
Pseudomonas sp.
Itaconate
competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit
Arthrobacter sp.
Itaconate
competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit
Pseudomonas sp.
malate
competitive inhibition
Pseudomonas sp.
Maleate
competitive inhibition
Pseudomonas sp.
malonate
competitive inhibition
Pseudomonas sp.
N-acetyl-DL-aspartate
-
Arthrobacter sp.
N-acetyl-DL-aspartate
competitive inhibition
Pseudomonas sp.
oxaloacetate
-
Arthrobacter sp.
oxaloacetate
competitive inhibition
Pseudomonas sp.
phosphate
-
Arthrobacter sp.
phosphate
competitive inhibition
Pseudomonas sp.
SO32-
inhibitory effect increases rapidly with time to a constant level, not competitve with substrate
Arthrobacter sp.
SO32-
inhibitory effect increases rapidly with time to a constant level, not competitve with substrate
Pseudomonas sp.
succinate
-
Arthrobacter sp.
succinate
competitive inhibition
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
(N-acetylaminomethylene)succinic acid
at pH 6.5 and 25°C
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32500
-
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-(acetamidomethylene)succinate + 2 H2O
Pseudomonas sp.
-
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
2-(acetamidomethylene)succinate + 2 H2O
Pseudomonas sp. IA
-
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.12
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp.
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
-
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp.
acetate + succinate semialdehyde + NH3 + CO2
same products as formed by acid hydrolysis
172028
Pseudomonas sp.
ir
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp. IA
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
-
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp. IA
acetate + succinate semialdehyde + NH3 + CO2
same products as formed by acid hydrolysis
172028
Pseudomonas sp. IA
ir
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
-
Arthrobacter sp.
monomer
SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
90
at pH 8 and 70°C stable for 1 min, loses activity completely in 1 min at 90°C
Pseudomonas sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.67
-
(N-acetylaminomethylene)succinic acid
-
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
pH 6.5 in 50 mM sodium phosphate, MES and HEPES buffers, pH 7.5 in Tris buffer
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
most efficient between pH 6.0 and 7.0
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
loses activity rapidly between 4.0 and 5.0
Pseudomonas sp.
Other publictions for EC 3.5.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735080
Zhang
How does (E)-2-(acetamidomethy ...
Mesorhizobium loti
PLoS ONE
8
e53811
2013
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2
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3
3
-
-
-
719527
Nagase
The mll6786 gene encodes a rep ...
Mesorhizobium loti
FEMS Microbiol. Lett.
329
116-122
2012
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3
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711220
McCulloch
Structure determination and ch ...
Mesorhizobium japonicum MAFF 303099
Biochemistry
49
1226-1235
2010
-
-
1
1
5
-
-
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-
1
-
1
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1
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1
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1
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1
5
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1
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1
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1
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-
1
1
-
-
-
710733
Kobayashi
Crystallization and preliminar ...
Mesorhizobium japonicum MAFF 303099
Acta Crystallogr. Sect. F
65
829-831
2009
-
-
1
1
-
-
-
-
-
-
1
1
-
3
-
-
1
-
-
-
-
-
1
1
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-
1
-
1
-
-
-
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-
1
1
-
-
-
1
-
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-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
685255
Mukherjee
PLP Catabolism: Identification ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
Biochemistry
47
6233-6241
2008
-
-
1
-
-
-
-
1
-
-
-
2
-
14
-
-
1
-
-
-
-
-
4
-
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-
1
-
-
-
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1
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1
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2
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1
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4
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1
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-
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-
699701
Yuan
Gene identification and charac ...
Mesorhizobium loti, Mesorhizobium loti MAFF303099
J. Nutr. Sci. Vitaminol.
54
185-190
2008
-
-
1
-
-
-
1
1
1
1
3
-
-
14
-
-
1
-
-
-
2
-
2
1
1
-
1
1
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
1
1
3
-
-
-
-
1
-
-
2
-
2
1
1
-
1
1
3
-
-
-
-
-
-
-
-
-
172028
Huynh
Enzymes of vitamin B6 degradat ...
Arthrobacter sp., Pseudomonas sp.
J. Biol. Chem.
260
2379-2383
1985
-
-
-
-
-
1
26
1
-
-
1
2
-
4
-
-
1
-
-
-
1
-
4
2
1
-
1
1
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
26
-
1
-
-
1
2
-
-
-
1
-
-
1
-
4
2
1
-
1
1
1
1
1
-
-
-
-
-
-
-
172029
Nyns
The bacterial oxidation of vit ...
Pseudomonas sp.
J. Biol. Chem.
244
2601-2605
1969
-
-
-
-
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-
1
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1
-
1
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1
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2
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1
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1
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1
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1
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1
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2
-
1
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1
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