Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.28 extracted from

  • Swift, S.M.; Seal, B.S.; Garrish, J.K.; Oakley, B.B.; Hiett, K.; Yeh, H.Y.; Woolsey, R.; Schegg, K.M.; Line, J.E.; Donovan, D.M.
    A thermophilic phage endolysin fusion to a Clostridium perfringens-specific cell wall binding domain creates an anti-Clostridium antimicrobial with improved thermostability (2015), Viruses, 7, 3019-3034 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.28

Application

Application Comment Organism
agriculture the recombinant thermostable chimeric endolysin can potentially be utilized as a feed additive to control the bacterium Clostridium perfringens during poultry production Clostridium phage phiCP26F
agriculture the recombinant thermostable chimeric endolysin can potentially be utilized as a feed additive to control the bacterium Clostridium perfringens during poultry production Geobacillus virus E2
drug development a thermophilic phage endolysin fusion to a Clostridium perfringens-specific cell wall binding domain creates an anti-Clostridium antimicrobial with improved thermostability, overview Clostridium phage phiCP26F
drug development a thermophilic phage endolysin fusion to a Clostridium perfringens-specific cell wall binding domain creates an anti-Clostridium antimicrobial with improved thermostability, overview Geobacillus virus E2

Cloned(Commentary)

Cloned (Comment) Organism
synthesis of a gene, codon optimized for Escherichia coli expression, that encodes the catalytic domain of the bacteriophage phiGVE2 amidase and the cell-wall binding (CWB) domain of the endolysin encoded by the genome of phiCP26F, recombinant expression of His-tagged chimeric enzyme PlyGVE2CpCWB in Escherichia coli strain BL21(DE3) Clostridium phage phiCP26F
synthesis of a gene, codon optimized for Escherichia coli expression, that encodes the catalytic domain of the bacteriophage phiGVE2 amidase and the cell-wall binding (CWB) domain of the endolysin encoded by the genome of phiCP26F, recombinant expression of His-tagged chimeric enzyme PlyGVE2CpCWB in Escherichia coli strain BL21(DE3) Geobacillus virus E2

Protein Variants

Protein Variants Comment Organism
additional information a codon optimized gene for the PlyGVE2 predicted N-acetylmuramoyl-L-alanine amidase endolysin domain (179 amino acids) of Geobacillus virus E2 page phiGVE2 is synthesized in-frame with the CWB domain (53 amino acids) of PlyCP26F from Clostridium perfringens-specific bacteriophage phiCP26F which is identical to the PlyCP39O endolysin CWB domain. The resulting protein, PlyGVE2CpCWB, lyses Clostridium perfringens in liquid and solid cultures Clostridium phage phiCP39-O
additional information a thermophilic phage endolysin fusion to a Clostridium perfringens-specific cell wall binding domain creates an anti-Clostridium antimicrobial with improved thermostability, overview. A codon optimized gene for the PlyGVE2 predicted N-acetylmuramoyl-L-alanine amidase endolysin domain (179 amino acids) from Geobacillus virus E2 page phiGVE2 is synthesized in-frame with the CWB domain (53 amino acids) of PlyCP26F from Clostridium perfringens-specific bacteriophage phiCP26F which is identical to the PlyCP39O endolysin CWB domain from Clostridium phage phiCP39-O. The resulting protein, PlyGVE2CpCWB, lyses Clostridium perfringens in liquid and solid cultures Clostridium phage phiCP26F
additional information a thermophilic phage endolysin fusion to a Clostridium perfringens-specific cell wall binding domain creates an anti-Clostridium antimicrobial with improved thermostability, overview. A codon optimized gene for the PlyGVE2 predicted N-acetylmuramoyl-L-alanine amidase endolysin domain (179 amino acids) from Geobacillus virus E2 page phiGVE2 is synthesized in-frame with the CWB domain (53 amino acids) of PlyCP26F from Clostridium perfringens-specific bacteriophage phiCP26F. The resulting protein, PlyGVE2CpCWB, lyses Clostridium perfringens in liquid and solid cultures Geobacillus virus E2

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl the recombinant chimeric enzyme PlyGVE2CpCWB shows full activity at 10 mM NaCl, 40% activity at 150 mM NaCl, and 16% active at 600 mM NaCl, pH 8.0 Clostridium phage phiCP26F
NaCl the recombinant chimeric enzyme PlyGVE2CpCWB shows full activity at 10 mM NaCl, 40% activity at 150 mM NaCl, and 16% active at 600 mM NaCl, pH 8.0 Geobacillus virus E2

Organism

Organism UniProt Comment Textmining
Clostridium phage phiCP26F F2VHX9
-
-
Clostridium phage phiCP39-O B6CXF7
-
-
Geobacillus virus E2 A6M970
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged chimeric enzyme PlyGVE2CpCWB from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Clostridium phage phiCP26F
recombinant His-tagged chimeric enzyme PlyGVE2CpCWB from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Geobacillus virus E2

Subunits

Subunits Comment Organism
? x * 27261, chimeric enzyme PlyGVE2CpCWB, sequence calculation, x * 27300, His-tagged chimeric enzyme PlyGVE2CpCWB, SDS-PAGE Clostridium phage phiCP26F
? x * 27261, chimeric enzyme PlyGVE2CpCWB, sequence calculation, x * 27300, His-tagged chimeric enzyme PlyGVE2CpCWB, SDS-PAGE Geobacillus virus E2

Synonyms

Synonyms Comment Organism
amidase-hydrolase UniProt Clostridium phage phiCP39-O
bacteriophage phiGVE2 amidase
-
 Geobacillus virus E2
endolysin
-
 Clostridium phage phiCP39-O
endolysin
-
 Clostridium phage phiCP26F
endolysin
-
 Geobacillus virus E2
phage endolysin
-
 Clostridium phage phiCP39-O
phage endolysin
-
 Clostridium phage phiCP26F
phi26F_gp22
-
 Clostridium phage phiCP26F
phiCP26F endolysin
-
 Clostridium phage phiCP26F
phiGVE2 endolysin
-
 Geobacillus virus E2
PlyCP26F
-
 Clostridium phage phiCP26F
PlyCP39O
-
 Clostridium phage phiCP39-O

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22 37 assay at Clostridium phage phiCP26F
22 37 assay at, chimeric enzyme PlyGVE2CpCWB Geobacillus virus E2
60
-
 wild-type N-acetylmuramoyl-L-alanine amidase Geobacillus virus E2

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 activity range, wild-type N-acetylmuramoyl-L-alanine amidase Geobacillus virus E2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 PlyGVE2CpCWB is more tolerant to heat treatment than PlyCP26F Clostridium phage phiCP26F
4 42 recombinant chimeric endolysin PlyGVE2CpCWB is completely stable for at least 30 min Geobacillus virus E2
4 22 PlyCP26F and PlyGVE2CpCWB are completely stable for at least 30 min Clostridium phage phiCP26F
37
-
 PlyCP26F loses roughly 10% of its activity after 30 min at 37°C, while PlyGVE2CpCWB is fully active Clostridium phage phiCP26F
42
-
 PlyCP26F loses roughly 40% of its activity after 30 min at 42°C, while PlyGVE2CpCWB is fully active Clostridium phage phiCP26F
50
-
 recombinant chimeric endolysin PlyGVE2CpCWB, over 95% activity remaining after 30 min Geobacillus virus E2
50
-
 recombinant chimeric endolysin PlyGVE2CpCWB, over 95% activity remaining after 30 min, PlyCP26F loses over 95% activity Clostridium phage phiCP26F
55
-
 recombinant chimeric endolysin PlyGVE2CpCWB, 57% activity remaining after 30 min Geobacillus virus E2
55
-
 recombinant chimeric endolysin PlyGVE2CpCWB, 57% activity remaining after 30 min, PlyCP26F loses over 95% activity Clostridium phage phiCP26F
65
-
 recombinant chimeric endolysin PlyGVE2CpCWB, 10% activity remaining after 30 min, inactivation of PlyCP26F Clostridium phage phiCP26F

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 recombinant isolated catalytic domain of PlyGVE2CpCWB Clostridium phage phiCP26F
6
-
 recombinant isolated catalytic domain of PlyGVE2CpCWB Geobacillus virus E2
8
-
 recombinant chimeric enzyme PlyGVE2CpCWB Clostridium phage phiCP26F
8
-
 recombinant chimeric enzyme PlyGVE2CpCWB Geobacillus virus E2

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, recombinant chimeric enzyme PlyGVE2CpCWB Clostridium phage phiCP26F
4 10 activity range, recombinant chimeric enzyme PlyGVE2CpCWB Geobacillus virus E2

General Information

General Information Comment Organism
physiological function PlyGVE2CpCWB chimeric mutant effectiveness in lysis against various bacteria, overview Geobacillus virus E2
physiological function the two N-acetylmuramoyl-L-alanine amidases from two bacteriophages, PhiCP26F and PhiCP39O, are identical in the C-terminal cell-wall binding domain, but have only 55% identity to each other in the N-terminal catalytic domain. Both endolysins, PlyCP26F and PlyCP39O, lyse their parental phage host strains of Clostridium perfringens as well as other strains of the bacterium when exposed externally, but do not lyse bacteria beyond the species Clostridium phage phiCP39-O
physiological function the two N-acetylmuramoyl-L-alanine amidases from two bacteriophages, PhiCP26F and PhiCP39O, are identical in the C-terminal cell-wall binding domain, but have only 55% identity to each other in the N-terminal catalytic domain. Both endolysins, PlyCP26F and PlyCP39O, lyse their parental phage host strains of Clostridium perfringens as well as other strains of the bacterium when exposed externally, but do not lyse bacteria beyond the species. PlyGVE2CpCWB chimeric mutant effectiveness in lysis against various bacteria, overview Clostridium phage phiCP26F