Literature summary for 3.5.1.28 extracted from

Young, B.F.; Roth, B.M.; Davies, C.
1H, 13C, and 15N resonance assignments of N-acetylmuramyl-L-alanine amidase (AmiC) N-terminal domain (NTD) from Neisseria gonorrhoeae (2019), Biomol. NMR Assign., 13, 63-66 .

Search for more literature for 3.5.1.28

Cloned(Commentary)

Cloned (Comment)	Organism
gene amiC, recombinant expression of N-terminally His6-tagged enzyme AmiC residues 29-159 fused to GB1 in Escherichia coli strain BL21(DE3), cells are grown in M9 minimal media containing 13C6-glucose and 15NH4Cl as carbon and nitrogen sources, respectively	Neisseria gonorrhoeae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of enzyme AmiC residues 29-159 fused to GB1, the AmiC-NTD construct contains four beta strands, followed by a single alpha-helix and then a further four beta-strands	Neisseria gonorrhoeae

Organism

Organism	UniProt	Comment	Textmining
Neisseria gonorrhoeae	Q5F6P7	-	-
Neisseria gonorrhoeae ATCC 700825	Q5F6P7	-	-
Neisseria gonorrhoeae FA 1090	Q5F6P7	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme AmiC residues 29-159 fused to GB1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by His6-TEV protease, and again nickel affinity chromatography	Neisseria gonorrhoeae

Synonyms

Synonyms	Comment	Organism
AmiC	-	Neisseria gonorrhoeae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Neisseria gonorrhoeae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Neisseria gonorrhoeae

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Release 2023.1 (January 2023)