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Literature summary for 3.5.1.28 extracted from
- Characterization of a novel cell wall binding domain-containing Staphylococcus aureus endolysin LysSA97 (2017), Appl. Microbiol. Biotechnol., 101, 147-158 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene SA97_036, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged cell wall binding domain CBD fused to GFP in Escherichia coli strain BL21(DE3) | Staphylococcus phage SA97 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | the enzyme is stable up to 300 mM and the activity only slightly affected, best without NaCl, 20% inhibition at 300 mM | Staphylococcus phage SA97 |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | the enzyme contains a cell wall binding domain | Staphylococcus phage SA97 | 5618 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus phage SA97 | A0A0F6N3N1 | i.e. Staphylococcus aureus phage SA97 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His-tagged cell wall binding domain CBD fused to GFP from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to near homogeneity | Staphylococcus phage SA97 |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the enzyme LysSA97 structure reveals a putative cell wall binding domain (CBD) as well as two enzymatically active domains (EADs) containing cysteine, histidine-dependent amidohydrolases/peptidases (CHAP, PF05257) and N-acetylmuramoyl-L-alanine amidase (amidase-3, PF01520) domains. The putative CBD of LysSA97 shows a specific binding spectrum against staphylococcal cells comparable to SH3 domain (PF08460) | Staphylococcus phage SA97 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endolysin | - |
Staphylococcus phage SA97 |
| LysSA97 | - |
Staphylococcus phage SA97 |
| SA97_036 | - |
Staphylococcus phage SA97 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
assay at | Staphylococcus phage SA97 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 65 | activity range, profile overview | Staphylococcus phage SA97 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
recombinant enzyme | Staphylococcus phage SA97 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 10 | activity range, profile overview | Staphylococcus phage SA97 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the putative cell wall binding domain (CBD) of LysSA97 shows a specific binding spectrum against staphylococcal cells comparable to SH3 domain (PF08460) | Staphylococcus phage SA97 |
| physiological function | enzyme LysSA97 specifically lyses the staphylococcal strains and effectively disrupts staphylococcal biofilms | Staphylococcus phage SA97 |
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