Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli LMG194 and B180 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 1 M LiCl, 10% (w/v) polyethylene glycol 6000 in a 0.1 M sodium citrate buffer (pH 4) for the native structure, and 1 M MgSO4 in a 0.1 M sodium acetate buffer (pH 4.6) for the Se-Met structure. The apoenzyme in complex with the substrate anhydro-N-acetylmuramic-acid-L-Ala-gamma-D-Glu-L-Lys is crystallized using 50% (w/v) polyethylene glycol 6000, 0.1 M sodium citrate buffer (pH 4), and 5 mM EDTA. The holoenzyme in complex with the product L-Ala-gamma-D-Glu-L-Lys is crystallized using 1 M MgCl2 in a 0.1 M sodium acetate buffer, pH 4.6 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P75820 | - |
- |
Purification (Comment) | Organism |
---|---|
S-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S100 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,6-anhydro-N-acetylmuramic-acid-L-Ala-gamma-D-Glu-L-Lys + H2O | - |
Escherichia coli | 1,6-anhydro-N-acetylmuramate + L-Ala-gamma-D-Glu-L-Lys | - |
? | |
additional information | the enzyme has no activity on MurNAc-L-Ala-D-Glu | Escherichia coli | ? | - |
? | |
N-acetylmuramoyl-L-alanine + H2O | - |
Escherichia coli | N-acetylmuramate + L-alanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AmiD | - |
Escherichia coli |