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Literature summary for 3.5.1.28 extracted from

  • Luetzner, N.; Paetzold, B.; Zoll, S.; Stehle, T.; Kalbacher, H.
    Development of a novel fluorescent substrate for Autolysin E, a bacterial type II amidase (2009), Biochem. Biophys. Res. Commun., 380, 554-558.
    View publication on PubMed
Search for more literature for 3.5.1.28

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Staphylococcus epidermidis
benzoyl-Arg-p-nitroanilide inhibition: moderately at 1 mM, completely at 10 mM Staphylococcus epidermidis
EDTA
-
 Staphylococcus epidermidis
Zn2+ 100 mM Staphylococcus epidermidis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2849
-
 7-methoxycoumarin-4-yl-acetyl-Ala-D-isoGln-Lys(2,4-dinitrophenyl)-D-Ala-Arg
-
 Staphylococcus epidermidis
0.3601
-
 7-methoxycoumarin-4-yl-acetyl-Ala-D-isoGlu-Lys(2,4-dinitrophenyl)-D-Ala-Arg
-
 Staphylococcus epidermidis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Staphylococcus epidermidis

Organism

Organism UniProt Comment Textmining
Staphylococcus epidermidis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-yl-acetyl-Ala-D-isoGln-Lys(2,4-dinitrophenyl)-D-Ala-Arg + H2O
-
 Staphylococcus epidermidis ?
-
 ?
7-methoxycoumarin-4-yl-acetyl-Ala-D-isoGlu-Lys(2,4-dinitrophenyl)-D-Ala-Arg + H2O
-
 Staphylococcus epidermidis ?
-
 ?

Synonyms

Synonyms Comment Organism
AmiE
-
 Staphylococcus epidermidis
autolysin E
-
 Staphylococcus epidermidis
N-acetylmuramoyl-L-alanine amidase
-
 Staphylococcus epidermidis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00159
-
 7-methoxycoumarin-4-yl-acetyl-Ala-D-isoGln-Lys(2,4-dinitrophenyl)-D-Ala-Arg
-
 Staphylococcus epidermidis
0.00163
-
 7-methoxycoumarin-4-yl-acetyl-Ala-D-isoGlu-Lys(2,4-dinitrophenyl)-D-Ala-Arg
-
 Staphylococcus epidermidis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Staphylococcus epidermidis

pH Range

pH Minimum pH Maximum Comment Organism
5.7 9 >80% activity Staphylococcus epidermidis