Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.28 extracted from

  • Romero, P.; Lopez, R.; Garcia, E.
    Characterization of LytA-like N-acetylmuramoyl-L-alanine amidases from two new Streptococcus mitis bacteriophages provides insights into the properties of the major pneumococcal autolysin (2004), J. Bacteriol., 186, 8229-8239.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.28

Cloned(Commentary)

Cloned (Comment) Organism
gene lytAB6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of the phage enzyme in Escherichia coli strain DH10B Streptococcus mitis
gene lytAHER, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of the phage enzyme in Escherichia coli strain DH10B Streptococcus mitis

Inhibitors

Inhibitors Comment Organism Structure
choline IC50: 0.4 mM; IC50: 2 mM Streptococcus mitis
deoxycholate at 1%; at 1% Streptococcus mitis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme posseses transmembrane segments Streptococcus mitis 16020
-
membrane the enzyme possesses transmembrane segments Streptococcus mitis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36782
-
 x * 36782, sequence calculation Streptococcus mitis
36919
-
 x * 36919, sequence calculation Streptococcus mitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus mitis the enzyme performs cell wall lysis ?
-
 ?
additional information Streptococcus mitis HER1055 the enzyme performs cell wall lysis ?
-
 ?
additional information Streptococcus mitis B6 the enzyme performs cell wall lysis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus mitis Q706F5 Streptococcus mitis bacteriophage PHIB6
-
Streptococcus mitis Q706F9 Streptococcus mitis bacteriophage PHIHER; isolated from Streptococcus mitis strain HER1055
-
Streptococcus mitis B6 Q706F5 Streptococcus mitis bacteriophage PHIB6
-
Streptococcus mitis HER1055 Q706F9 Streptococcus mitis bacteriophage PHIHER; isolated from Streptococcus mitis strain HER1055
-

Purification (Commentary)

Purification (Comment) Organism
recombinant phage enzyme from Escherichia coli Streptococcus mitis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000044
-
-
 Streptococcus mitis
0.0000046
-
-
 Streptococcus mitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme performs cell wall lysis Streptococcus mitis ?
-
 ?
additional information the enzyme performs cell wall lysis Streptococcus mitis HER1055 ?
-
 ?
additional information the enzyme performs cell wall lysis Streptococcus mitis B6 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36782, sequence calculation Streptococcus mitis
? x * 36919, sequence calculation Streptococcus mitis

Synonyms

Synonyms Comment Organism
LytA-like N-acetylmuramoyl-L-alanine amidase
-
 Streptococcus mitis
LytAB6
-
 Streptococcus mitis
LytAHER
-
 Streptococcus mitis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Streptococcus mitis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.4
-
 IC50: 0.4 mM Streptococcus mitis choline
2
-
 IC50: 2 mM Streptococcus mitis choline