Cloned (Comment) | Organism |
---|---|
gene PGRP-SB1, DNA and amino acid sequence determination and analysis, expression of His6-tagged 128 C-terminal residues and of his6-tagged 180 N-terminal residues in Escherichia coli strain XL-1I Blue | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inactivation through depletion of zinc ions | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | absolutely required for activity | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Drosophila melanogaster | the enzyme performs cell wall lysis, and shows antibacterial activity against Bacillus megaterium | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q70PY2 | gene PGRP-SB1 or CG9681; gene PGRP-SB1 or CG9681 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
S2 cell | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme performs cell wall lysis, and shows antibacterial activity against Bacillus megaterium | Drosophila melanogaster | ? | - |
? | |
N-acetylmuramoyl-L-alanine + H2O | the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, peptidoglycan substrate from Escherichia coli, PGRP-SB1 is highly active against peptidoglycans that have a diaminopimelic acid residue in the cross-linking peptide, but lacks activity to most lysine-containing peptidoglycans | Drosophila melanogaster | N-acetylmuramate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the peptidoglycan recognition protein, PGRP, family | Drosophila melanogaster |
PGRP-SB1 | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 25 | assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Drosophila melanogaster |