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Literature summary for 3.5.1.28 extracted from

  • Wang, Z.M.; Li, X.; Cocklin, R.R.; Wang, M.; Fukase, K.; Inamura, S.; Kusumoto, S.; Gupta, D.; Dziarski, R.
    Human peptidoglycan recognition protein-L is an N-acetylmuramoyl-L-alanine amidase (2003), J. Biol. Chem., 278, 49044-49052.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cells and in HepG2/C3A human hepatoblastoma cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C419A inactive mutant Homo sapiens
C530S inactive mutant Homo sapiens
H411A mutant with full amidase activity Homo sapiens
H436A mutant with full amidase activity Homo sapiens
additional information N-terminal DELTA1-343 deletion mutant of PGRP-L with reduced activity, inactive C-terminal DELTA344-576 deletion mutant of PGRP-L Homo sapiens
W442A mutant with reduced amidase activity Homo sapiens
Y447A inactive mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition, restored by 1 mM Zn2+, partially restored by 10 mM Mg2+ Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface recombinant PGRP-L shows primarily intracellular and cell surface location Homo sapiens 9986
-
intracellular recombinant PGRP-L shows primarily intracellular and cell surface location Homo sapiens 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ Zn2+-dependent Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96PD5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver PGRP-L, mainly in Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GlcNAc-MurNAc-L-Ala-D-isoGln-L-Lys-D-Ala)2 + H2O
-
Homo sapiens ?
-
?
biotin-peptidoglucan + H2O
-
Homo sapiens ?
-
?
additional information PGRP-L has no direct bacteriolytic activity for intact bacteria, not: MurNAc-L-Ala-D-isoGln, GlcNAc-MurNAc-L-Ala-D-isoGln Homo sapiens ?
-
?
MurNAc-L-Ala-D-isoGln-L-Lys + H2O MurNAc-tripeptide, minimum peptidoglycan fragment hydrolyzed by PGRP-L Homo sapiens MurNAc + L-Ala-D-isoGln-L-Lys
-
?
MurNAc-L-Ala-D-isoGln-L-Lys-D-Ala + H2O
-
Homo sapiens MurNAc + L-Ala-D-isoGln-L-Lys-D-Ala
-
?
peptidoglucan + H2O PGRP-L hydrolyzes the amide bond between MurNAc and L-Ala of peptidoglycan, Cys-419 is required for the amidase activity, polymeric soluble uncross-linked peptidoglycan from Staphylococcus aureus, digest products Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
peptidoglycan recognition protein-L
-
Homo sapiens
PGRP-L
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens