Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | strong, 5 mM, 94% inhibition | Achromobacter lyticus | |
dithiothreitol | 5 mM, 53% inhibition | Achromobacter lyticus | |
EDTA | less inhibitory than 1,10-phenanthroline, 10 mM, 25% inhibition | Achromobacter lyticus | |
additional information | not inhibited by diisopropylfluorophosphate, iodoacetic acid, Zn2+, Ca2+, Mg2+, each at 5 mM | Achromobacter lyticus | |
NaCl | sensitive to salt concentration, loses its lytic activity in 10 mM Tris-HCl containing 100 mM NaCl | Achromobacter lyticus | |
Tris-HCl | lytic activity in 30 mM Tris-HCl is only 30% of that in 10 mM solution, almost inactive in 70 mM Tris-HCl | Achromobacter lyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | probably a metalloenzyme, no effect on lytic activity by Zn2+, Ca2+ or Mg2+, each at 5 mM | Achromobacter lyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19396 | - |
1 * 19396, sequence calculation | Achromobacter lyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Achromobacter lyticus | bacteriolytic/cell wall hydrolytic amidase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter lyticus | P81717 | - |
- |
Purification (Comment) | Organism |
---|---|
from achromopeptidase | Achromobacter lyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | bacteriolytic/cell wall hydrolytic amidase | Achromobacter lyticus | ? | - |
? | |
peptidoglucan + H2O | ChwA primarily hydrolyzes the N-acetylmuramoyl-L-alanyl amide bond, splits the linkage between polysaccharides and peptides, bacteriolytic/cell wall hydrolytic amidase, CwhA lyses CHCl3-treated Escherichia coli JM109 most efficiently, followed by Micrococcus luteus, Staphylococcus aureus IFO 13276, Enterococcus faecalis IFO 3971, Pediococcus acidilactici IFO3385 and intact Escherichia coli JM109 | Achromobacter lyticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 19396, sequence calculation | Achromobacter lyticus |
Synonyms | Comment | Organism |
---|---|---|
CwhA | cell wall hydrolytic amidase | Achromobacter lyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Achromobacter lyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Achromobacter lyticus |