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Literature summary for 3.5.1.28 extracted from

  • Li, S.; Norioka, S.; Sakiyama, F.
    Purification, characterization, and primary structure of a novel cell wall hydrolytic amidase, CwhA, from Achromobacter lyticus (2000), J. Biochem., 127, 1033-1039.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline strong, 5 mM, 94% inhibition Achromobacter lyticus
dithiothreitol 5 mM, 53% inhibition Achromobacter lyticus
EDTA less inhibitory than 1,10-phenanthroline, 10 mM, 25% inhibition Achromobacter lyticus
additional information not inhibited by diisopropylfluorophosphate, iodoacetic acid, Zn2+, Ca2+, Mg2+, each at 5 mM Achromobacter lyticus
NaCl sensitive to salt concentration, loses its lytic activity in 10 mM Tris-HCl containing 100 mM NaCl Achromobacter lyticus
Tris-HCl lytic activity in 30 mM Tris-HCl is only 30% of that in 10 mM solution, almost inactive in 70 mM Tris-HCl Achromobacter lyticus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information probably a metalloenzyme, no effect on lytic activity by Zn2+, Ca2+ or Mg2+, each at 5 mM Achromobacter lyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19396
-
1 * 19396, sequence calculation Achromobacter lyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Achromobacter lyticus bacteriolytic/cell wall hydrolytic amidase ?
-
?

Organism

Organism UniProt Comment Textmining
Achromobacter lyticus P81717
-
-

Purification (Commentary)

Purification (Comment) Organism
from achromopeptidase Achromobacter lyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information bacteriolytic/cell wall hydrolytic amidase Achromobacter lyticus ?
-
?
peptidoglucan + H2O ChwA primarily hydrolyzes the N-acetylmuramoyl-L-alanyl amide bond, splits the linkage between polysaccharides and peptides, bacteriolytic/cell wall hydrolytic amidase, CwhA lyses CHCl3-treated Escherichia coli JM109 most efficiently, followed by Micrococcus luteus, Staphylococcus aureus IFO 13276, Enterococcus faecalis IFO 3971, Pediococcus acidilactici IFO3385 and intact Escherichia coli JM109 Achromobacter lyticus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 19396, sequence calculation Achromobacter lyticus

Synonyms

Synonyms Comment Organism
CwhA cell wall hydrolytic amidase Achromobacter lyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Achromobacter lyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Achromobacter lyticus