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Literature summary for 3.5.1.28 extracted from

  • Singer, H.J.; Wise, E.M.; Park, J.T.
    Properties and purification of N-acetylmuramyl-L-alanine amidase from Staphylococcus aureus H (1972), J. Bacteriol., 112, 932-939.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
ammonium acetate 18% activation at 0.15 M Staphylococcus aureus
ammonium sulfate 20% activation at 0.15 M Staphylococcus aureus
NH4Cl 8% activation at 0.15 M Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Staphylococcus aureus
EDTA
-
 Staphylococcus aureus
K+
-
 Staphylococcus aureus
Na+
-
 Staphylococcus aureus
p-chloromercuribenzene sulfonate
-
 Staphylococcus aureus
Pb2+
-
 Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
800000 1000000 sucrose density gradient centrifugation, gel filtration Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Storage Stability

Storage Stability Organism
-20°C or 4°C, overnight, in glass tubes, total loss of activity Staphylococcus aureus
-20°C, crude cell extract, 2 years, 20% loss of activity Staphylococcus aureus
-20°C, overnight, in polycarbonate tubes, 25-50% loss of activity Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
murein + H2O also murein extracted from cells grown in the presence of penicillin, N-acetylated murein Staphylococcus aureus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 10 min at 25°C result in 25% loss of inactivation Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Staphylococcus aureus