Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.26 extracted from

  • Noronkoski, T.; Stoineva, I.B.; Petkov, D.D.; Mononen, I.
    Recombinant human glycosylasparaginase catalyzes hydrolysis of L-asparagine (1997), FEBS Lett., 412, 149-152.
    View publication on PubMed
Search for more literature for 3.5.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in NIH-3T3 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-Diazo-4-oxo-L-norvaline
-
 Homo sapiens
L-asparagine competitive inhibition, Ki: 0.454 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.208
-
 N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine
-
 Homo sapiens
0.66
-
 L-asparagine
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Homo sapiens 21% activity compared to N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine as substrate L-aspartate + NH3
-
 r
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O Homo sapiens involved in degradation of glycoproteins 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, recombinant enzyme Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.34
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O 21% activity compared to N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine as substrate Homo sapiens L-aspartate + NH3
-
 r
L-aspartic acid beta-(7-amido-4-methylcoumarin) + H2O
-
 Homo sapiens 7-amino-4-methylcoumarin + L-aspartate
-
 ?
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O involved in degradation of glycoproteins Homo sapiens 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate
-
 r

Synonyms

Synonyms Comment Organism
glycosylasparaginase
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 optimal activity within Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.454
-
 L-asparagine competitive inhibition Homo sapiens