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Literature summary for 3.5.1.24 extracted from
- A highly active bile salt hydrolase from Enterococcus faecalis shows positive cooperative kinetics (2016), Process Biochem., 51, 263-269 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect on enzyme activity by EDTA | Enterococcus faecalis | |
| penicilin V | Pen V, is expected to cause competitive inhibition of BSH activity, but EfBSH shows enhanced activity towards glycodeoxycholic acid in the presence of Pen V, and no inhibition is observed, contrary to the expected results | Enterococcus faecalis |  |
| Triton X-100 | slightly activating | Enterococcus faecium | |
| Triton X-100 | slightly activating | Enterococcus faecalis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bsh, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 | Enterococcus faecalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition | Enterococcus faecalis | |
| Cu2+ | moderate inhibition | Enterococcus faecalis | |
| Hg2+ | complete inhibition | Enterococcus faecalis | |
| additional information | no effect on enzyme activity by EDTA. Penicilin V (Pen V) is expected to cause competitive inhibition of BSH activity, but EfBSH shows enhanced activity towards glycodeoxycholic acid in the presence of Pen V, and no inhibition is observed, contrary to the expected results | Enterococcus faecalis | |
| Penicillin V | Pen V, modulating effect of the non-substrate ligand Pen V on the hydrolysing ability of EfBSH towards bile acid such as glycodeoxycholic acid, competitive inhibition of BSH activity by Pen V | Enterococcus faecium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the enzyme demonstrates unique enzyme kinetics of non-linear regression, thereby displaying positive cooperativity | Enterococcus faecium | |
| additional information | - |
additional information | the enzyme demonstrates unique enzyme kinetics of non-linear regression, thereby displaying positive cooperativity, steady-state kinetics | Enterococcus faecalis | |
| 4.49 | - |
taurochenodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 9.28 | - |
taurodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 9.94 | - |
glycodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 11.07 | - |
glycocholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 13.16 | - |
taurocholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 16.97 | - |
glycochenodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slightly activating | Enterococcus faecalis | |
| Co2+ | slightly activating | Enterococcus faecalis | |
| Mg2+ | slightly activating | Enterococcus faecalis | |
| Mn2+ | slightly activating | Enterococcus faecalis | |
| Ni2+ | slightly activating | Enterococcus faecalis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholic acid + H2O | Enterococcus faecalis | - |
chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | Enterococcus faecalis NCIM 2403 | - |
chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | Enterococcus faecalis T2 | - |
chenodeoxycholate + glycine | - |
? | |
| glycocholic acid + H2O | Enterococcus faecium | - |
cholate + glycine | - |
? | |
| glycocholic acid + H2O | Enterococcus faecalis | - |
cholate + glycine | - |
? | |
| glycocholic acid + H2O | Enterococcus faecalis NCIM 2403 | - |
cholate + glycine | - |
? | |
| glycocholic acid + H2O | Enterococcus faecalis T2 | - |
cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Enterococcus faecium | - |
deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Enterococcus faecalis | - |
deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Enterococcus faecalis NCIM 2403 | - |
deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Enterococcus faecalis T2 | - |
deoxycholate + glycine | - |
? | |
| taurochenodeoxycholic acid + H2O | Enterococcus faecalis | - |
chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | Enterococcus faecalis NCIM 2403 | - |
chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | Enterococcus faecalis T2 | - |
chenodeoxycholate + taurine | - |
? | |
| taurocholic acid + H2O | Enterococcus faecalis | - |
cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Enterococcus faecalis | - |
deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Enterococcus faecalis NCIM 2403 | - |
deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Enterococcus faecalis T2 | - |
deoxycholate + taurine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | C7CXJ5 | - |
- |
| Enterococcus faecalis NCIM 2403 | C7CXJ5 | - |
- |
| Enterococcus faecalis T2 | C7CXJ5 | - |
- |
| Enterococcus faecium | Q83YZ2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration | Enterococcus faecalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1290 | - |
substrate glycodeoxycholic acid, pH 5.0, 50°C, purified recombinant enzyme | Enterococcus faecium |
| 1290 | - |
substrate glycodeoxycholic acid, pH 5.0, 50°C, purified recombinant enzyme | Enterococcus faecalis |
| 1390 | - |
substrate glycocholic acid, pH 5.0, 50°C, purified recombinant enzyme | Enterococcus faecium |
| 1390 | - |
substrate glycocholic acid, pH 5.0, 50°C, purified recombinant enzyme | Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholic acid + H2O | - |
Enterococcus faecalis | chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | - |
Enterococcus faecalis NCIM 2403 | chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | - |
Enterococcus faecalis T2 | chenodeoxycholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Enterococcus faecium | cholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Enterococcus faecalis | cholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Enterococcus faecalis NCIM 2403 | cholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Enterococcus faecalis T2 | cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Enterococcus faecium | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Enterococcus faecalis | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Enterococcus faecalis NCIM 2403 | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Enterococcus faecalis T2 | deoxycholate + glycine | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Enterococcus faecalis | chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Enterococcus faecalis NCIM 2403 | chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Enterococcus faecalis T2 | chenodeoxycholate + taurine | - |
? | |
| taurocholic acid + H2O | - |
Enterococcus faecalis | cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Enterococcus faecalis | deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Enterococcus faecalis NCIM 2403 | deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Enterococcus faecalis T2 | deoxycholate + taurine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000-30000, about, recombinant enzyme, SDS-PAGE | Enterococcus faecalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bile salt hydrolase | - |
Enterococcus faecium |
| bile salt hydrolase | - |
Enterococcus faecalis |
| BSH | - |
Enterococcus faecium |
| BSH | - |
Enterococcus faecalis |
| cholylglycine hydrolase | - |
Enterococcus faecium |
| cholylglycine hydrolase | - |
Enterococcus faecalis |
| EfBSH | - |
Enterococcus faecium |
| EfBSH | - |
Enterococcus faecalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Enterococcus faecium |
| 50 | - |
assay at | Enterococcus faecalis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | over 50% of maximal activity within this range | Enterococcus faecalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 40 | purified recombinant enzyme, 3 h, about 80% activity remaining | Enterococcus faecalis |
| 50 | - |
purified recombinant enzyme, 3 h, about 65% activity remaining | Enterococcus faecalis |
| 50 | - |
purified recombinant enzyme, considerably stable at | Enterococcus faecium |
| 60 | - |
purified recombinant enzyme, 3 h, inactivation | Enterococcus faecalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2260 | - |
glycochenodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 2500 | - |
taurocholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 2600 | - |
taurodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 3013 | - |
glycodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 3250 | - |
glycocholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 6210 | - |
taurochenodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Enterococcus faecium |
| 5 | - |
assay at | Enterococcus faecalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | over 50% of maximal activity within this range | Enterococcus faecalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | purified recombinant enzyme, considerably stable at | Enterococcus faecium |
| 5 | 8 | purified recombinant enzyme, about 80% activity remaining | Enterococcus faecalis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the active-site residues Cys2, Arg16, Asp19,Asn79, Asn170, and Arg223 are conserved | Enterococcus faecalis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 133.2 | - |
glycochenodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 190 | - |
taurocholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 280.2 | - |
taurodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 293.6 | - |
glycocholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 303.1 | - |
glycodeoxycholic acid | pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
| 1383.1 | - |
taurochenodeoxycholic acid | about, pH 5.0, 50°C, recombinant enzyme | Enterococcus faecalis | |
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