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Literature summary for 3.5.1.24 extracted from
- Bile-salt-hydrolases from the probiotic strain Lactobacillus johnsonii La1 mediate anti-giardial activity in vitro and in vivo (2018), Front. Microbiol., 8, 2707 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | active BSH derived from the probiotic Lactobacillus johnsonii strain La1 may yield anti-giardial effects in vitro and in vivo. The enzyme may be useful in approaches for the treatment of widely spread but neglected infectious disease, such as giardiasis, both in human and in veterinary medicine | Lactobacillus johnsonii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bsh12 or LJ1412, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of His-tagged enzyme in Escherichia coli strain SE1, subcloning in Escherichia coli strain CYS21 | Lactobacillus johnsonii |
| gene bsh47 or LJ1147, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of His-tagged enzyme in Escherichia coli strain SE1, subcloning in Escherichia coli strain CYS21 | Lactobacillus johnsonii |
| gene bsh56 or LJ1156, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of His-tagged enzyme in Escherichia coli strain SE1, subcloning in Escherichia coli strain CYS21 | Lactobacillus johnsonii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycocholic acid + H2O | Lactobacillus johnsonii | - |
cholate + glycine | - |
? |  |
| glycocholic acid + H2O | Lactobacillus johnsonii La1 | - |
cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Lactobacillus johnsonii | - |
deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Lactobacillus johnsonii La1 | - |
deoxycholate + glycine | - |
? | |
| additional information | Lactobacillus johnsonii | Escherichia coli strain SE1 expressing recombinant BSH56 efficiently hydrolyzes both tauro- and glyco-conjugated bile salts | ? | - |
? | |
| additional information | Lactobacillus johnsonii | recombinant BSH47 efficiently hydrolyzes tauro-conjugated bile salts, whereas no BSH activity is detected for glyco-conjugated bile salts in transformed Escherichia coli strain SE1 | ? | - |
? | |
| additional information | Lactobacillus johnsonii La1 | recombinant BSH47 efficiently hydrolyzes tauro-conjugated bile salts, whereas no BSH activity is detected for glyco-conjugated bile salts in transformed Escherichia coli strain SE1 | ? | - |
? | |
| additional information | Lactobacillus johnsonii La1 | Escherichia coli strain SE1 expressing recombinant BSH56 efficiently hydrolyzes both tauro- and glyco-conjugated bile salts | ? | - |
? | |
| taurocholic acid + H2O | Lactobacillus johnsonii | - |
cholate + taurine | - |
? | |
| taurocholic acid + H2O | Lactobacillus johnsonii La1 | - |
cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Lactobacillus johnsonii | - |
deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Lactobacillus johnsonii La1 | - |
deoxycholate + taurine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus johnsonii | Q74IV4 | - |
- |
| Lactobacillus johnsonii | Q74JG0 | - |
- |
| Lactobacillus johnsonii | Q74LX7 | - |
- |
| Lactobacillus johnsonii La1 | Q74IV4 | - |
- |
| Lactobacillus johnsonii La1 | Q74JG0 | - |
- |
| Lactobacillus johnsonii La1 | Q74LX7 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycocholic acid + H2O | - |
Lactobacillus johnsonii | cholate + glycine | - |
? | |
| glycocholic acid + H2O | low activity | Lactobacillus johnsonii | cholate + glycine | - |
? | |
| glycocholic acid + H2O | low activity | Lactobacillus johnsonii La1 | cholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Lactobacillus johnsonii La1 | cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Lactobacillus johnsonii | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | low activity | Lactobacillus johnsonii | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | low activity | Lactobacillus johnsonii La1 | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Lactobacillus johnsonii La1 | deoxycholate + glycine | - |
? | |
| additional information | Escherichia coli strain SE1 expressing recombinant BSH56 efficiently hydrolyzes both tauro- and glyco-conjugated bile salts | Lactobacillus johnsonii | ? | - |
? | |
| additional information | recombinant BSH47 efficiently hydrolyzes tauro-conjugated bile salts, whereas no BSH activity is detected for glyco-conjugated bile salts in transformed Escherichia coli strain SE1 | Lactobacillus johnsonii | ? | - |
? | |
| additional information | no significant enzymatic activity is detected for BSH12 with glycocholic acid, taurocholic acid, glycodeoxycholic acid, and taurodeoxycholic acid | Lactobacillus johnsonii | ? | - |
? | |
| additional information | no significant enzymatic activity is detected for BSH12 with glycocholic acid, taurocholic acid, glycodeoxycholic acid, and taurodeoxycholic acid | Lactobacillus johnsonii La1 | ? | - |
? | |
| additional information | recombinant BSH47 efficiently hydrolyzes tauro-conjugated bile salts, whereas no BSH activity is detected for glyco-conjugated bile salts in transformed Escherichia coli strain SE1 | Lactobacillus johnsonii La1 | ? | - |
? | |
| additional information | Escherichia coli strain SE1 expressing recombinant BSH56 efficiently hydrolyzes both tauro- and glyco-conjugated bile salts | Lactobacillus johnsonii La1 | ? | - |
? | |
| taurocholic acid + H2O | - |
Lactobacillus johnsonii | cholate + taurine | - |
? | |
| taurocholic acid + H2O | preferred substrate | Lactobacillus johnsonii | cholate + taurine | - |
? | |
| taurocholic acid + H2O | - |
Lactobacillus johnsonii La1 | cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Lactobacillus johnsonii | deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Lactobacillus johnsonii La1 | deoxycholate + taurine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35800, recombinant enzyme, SDS-PAGE | Lactobacillus johnsonii |
| ? | x * 37100, recombinant enzyme, SDS-PAGE | Lactobacillus johnsonii |
| ? | x * 37400, recombinant enzyme, SDS-PAGE | Lactobacillus johnsonii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bile salt hydrolase | - |
Lactobacillus johnsonii |
| Bile-Salt-Hydrolase | - |
Lactobacillus johnsonii |
| BSH12 | - |
Lactobacillus johnsonii |
| BSH47 | - |
Lactobacillus johnsonii |
| BSH56 | - |
Lactobacillus johnsonii |
| LJ1412 | - |
Lactobacillus johnsonii |
| LJ_0056 | - |
Lactobacillus johnsonii |
| LJ_1147 | - |
Lactobacillus johnsonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Lactobacillus johnsonii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Lactobacillus johnsonii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | bile salt hydrolase (BSH)-like activities from the probiotic Lactobacillus johnsonii strain La1 may contribute to the anti-giardial activity displayed by this strain. Giardia duodenalis (syn. G. lamblia, G. intestinalis) is the protozoan parasite responsible for giardiasis, themost common and widely spread intestinal parasitic disease worldwide, affecting both humans and animals. After cysts ingestion (through either contaminated food or water), Giardia excysts in the upper intestinal tract to release replicating trophozoites that are responsible for the production of symptoms | Lactobacillus johnsonii |
| physiological function | bile salt hydrolase (BSH)-like activities from the probiotic Lactobacillus johnsonii strain La1 may contribute to the anti-giardial activity displayed by this strain. Giardia duodenalis (syn. G. lamblia, G. intestinalis) is the protozoan parasite responsible for giardiasis, themost common and widely spread intestinal parasitic disease worldwide, affecting both humans and animals. After cysts ingestion (through either contaminated food or water), Giardia excysts in the upper intestinal tract to release replicating trophozoites that are responsible for the production of symptoms. Isozyme BSH47 exerts significant anti-giardial effects when tested in a murine model of giardiasis via Giardia duodenalis strain WB6 | Lactobacillus johnsonii |
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