Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | slight activation | Lactobacillus gasseri | |
butanol | - |
Lactobacillus gasseri | |
DTT | slight activation at 10 mM | Lactobacillus gasseri | |
Isopropanol | - |
Lactobacillus gasseri | |
Triton X-100 | - |
Lactobacillus gasseri | |
Tween 80 | - |
Lactobacillus gasseri |
Cloned (Comment) | Organism |
---|---|
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Eschericchia coli strain DH5alpha | Lactobacillus gasseri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ampicillin | almost complete inhibition | Lactobacillus gasseri | |
ascorbic acid | moderate inhibition | Lactobacillus gasseri | |
CaCl2 | slight inhibition | Lactobacillus gasseri | |
CuCl2 | strong inhibition | Lactobacillus gasseri | |
CuSO4 | strong inhibition | Lactobacillus gasseri | |
doxycycline hydrochloride | almost complete inhibition | Lactobacillus gasseri | |
erythromycin | moderate inhibition | Lactobacillus gasseri | |
ethanol | slight inhibition | Lactobacillus gasseri | |
KIO3 | strong inhibition | Lactobacillus gasseri | |
Lincomycin | slight inhibition | Lactobacillus gasseri | |
methanol | slight inhibition | Lactobacillus gasseri | |
MgCl2 | - |
Lactobacillus gasseri | |
MgSO4 | - |
Lactobacillus gasseri | |
MnCl2 | - |
Lactobacillus gasseri | |
MnSO4 | - |
Lactobacillus gasseri | |
additional information | chloroform, 2-mercaptoethanol and EDTA show poor effects on the enzyme activity. Proteinase K almost completely abolishes the enzyme activity, strong inhibition by pepsin. The enzyme is not affected by alpha-amylase and catalase, and only slightly by lysozyme | Lactobacillus gasseri | |
NaHIO3 | strong inhibition | Lactobacillus gasseri | |
neomycin | strong inhibition | Lactobacillus gasseri | |
oxytetracycline | almost complete inhibition | Lactobacillus gasseri | |
Penicillin V | almost complete inhibition | Lactobacillus gasseri | |
riboflavin | almost complete inhibition | Lactobacillus gasseri | |
SDS | almost complete inhibition | Lactobacillus gasseri | |
ZnCl2 | - |
Lactobacillus gasseri | |
ZnSO4 | - |
Lactobacillus gasseri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycocholate + H2O | Lactobacillus gasseri | - |
cholate + glycine | - |
? | |
glycocholate + H2O | Lactobacillus gasseri FR4 | - |
cholate + glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus gasseri | B9V405 | - |
- |
Lactobacillus gasseri FR4 | B9V405 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactobacillus gasseri |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycocholate + H2O | - |
Lactobacillus gasseri | cholate + glycine | - |
? | |
glycocholate + H2O | - |
Lactobacillus gasseri FR4 | cholate + glycine | - |
? | |
additional information | purified recombinant LgBSH exhibits substrate specificity toward glyco-conjugated bile salts (GCA and GDCA) compared to tauro-conjugated bile salts. Substrate specificity of LgBSH, overview | Lactobacillus gasseri | ? | - |
? | |
additional information | purified recombinant LgBSH exhibits substrate specificity toward glyco-conjugated bile salts (GCA and GDCA) compared to tauro-conjugated bile salts. Substrate specificity of LgBSH, overview | Lactobacillus gasseri FR4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, recombinant enzyme, SDS-PAGE | Lactobacillus gasseri |
Synonyms | Comment | Organism |
---|---|---|
bile salt hydrolase | - |
Lactobacillus gasseri |
BSH | - |
Lactobacillus gasseri |
LgBSH | - |
Lactobacillus gasseri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
recombinant enzyme | Lactobacillus gasseri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range, profile overview | Lactobacillus gasseri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Lactobacillus gasseri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | activity range, profile overview | Lactobacillus gasseri |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme structure homology model of LgBSH using the structure of Enterococcus faecalis BSH (PDB ID 4WL3) as a template. Residues involved in catalysis are identified based on the superimposed structure, including Cys1, Arg16, Asp19, Asn79, Asn171, and Arg224. Molecular docking analysis of ligands | Lactobacillus gasseri |