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Literature summary for 3.5.1.24 extracted from
- Molecular cloning, characterization, and comparison of four bile salt hydrolase-related enzymes from Lactobacillus plantarum GD2 of human origin (2018), Food Biotechnol., 32, 191-205 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bsh1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL-1 Blue | Lactiplantibacillus plantarum |
| gene bsh2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL-1 Blue | Lactiplantibacillus plantarum |
| gene bsh3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL-1 Blue | Lactiplantibacillus plantarum |
| gene bsh4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL-1 Blue | Lactiplantibacillus plantarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholic acid + H2O | Lactiplantibacillus plantarum | - |
chenodeoxycholate + glycine | - |
? |  |
| glycochenodeoxycholic acid + H2O | Lactiplantibacillus plantarum GD2 | - |
chenodeoxycholate + glycine | - |
? | |
| glycocholic acid + H2O | Lactiplantibacillus plantarum | - |
cholate + glycine | - |
? | |
| glycocholic acid + H2O | Lactiplantibacillus plantarum GD2 | - |
cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Lactiplantibacillus plantarum | - |
deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | Lactiplantibacillus plantarum GD2 | - |
deoxycholate + glycine | - |
? | |
| taurochenodeoxycholic acid + H2O | Lactiplantibacillus plantarum | - |
chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | Lactiplantibacillus plantarum GD2 | - |
chenodeoxycholate + taurine | - |
? | |
| taurocholic acid + H2O | Lactiplantibacillus plantarum | - |
cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Lactiplantibacillus plantarum | - |
deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | Lactiplantibacillus plantarum GD2 | - |
deoxycholate + taurine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum | A0A193H6Q2 | isolated from human gut or faeces | - |
| Lactiplantibacillus plantarum | A0A1B1FMJ8 | isolated from human gut or faeces | - |
| Lactiplantibacillus plantarum | A0A1I9RYI4 | isolated from human gut or faeces | - |
| Lactiplantibacillus plantarum | KU961675 | isolated from human gut or faeces | - |
| Lactiplantibacillus plantarum GD2 | A0A193H6Q2 | isolated from human gut or faeces | - |
| Lactiplantibacillus plantarum GD2 | A0A1B1FMJ8 | isolated from human gut or faeces | - |
| Lactiplantibacillus plantarum GD2 | A0A1I9RYI4 | isolated from human gut or faeces | - |
| Lactiplantibacillus plantarum GD2 | KU961675 | isolated from human gut or faeces | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycochenodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | chenodeoxycholate + glycine | - |
? | |
| glycochenodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum GD2 | chenodeoxycholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Lactiplantibacillus plantarum | cholate + glycine | - |
? | |
| glycocholic acid + H2O | best substrate | Lactiplantibacillus plantarum | cholate + glycine | - |
? | |
| glycocholic acid + H2O | - |
Lactiplantibacillus plantarum GD2 | cholate + glycine | - |
? | |
| glycocholic acid + H2O | best substrate | Lactiplantibacillus plantarum GD2 | cholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | best substrate | Lactiplantibacillus plantarum | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum GD2 | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | best substrate | Lactiplantibacillus plantarum GD2 | deoxycholate + glycine | - |
? | |
| additional information | isozyme BSH1 shows very high overall activity compared to the other BSH isozymes from Lactobacillus plantarum strain GD2. Substrate specificity, overview | Lactiplantibacillus plantarum | ? | - |
? | |
| additional information | isozyme BSH2 shows very low overall activity compared to the other BSH isozymes from Lactobacillus plantarum strain GD2. Substrate specificity, overview. Activity with taurodeoxycholic acid and taurochenodeoxycholic acid is below detection limit | Lactiplantibacillus plantarum | ? | - |
? | |
| additional information | isozyme BSH3 activity compared to the other BSH isozymes from Lactobacillus plantarum strain GD2, substrate specificity, overview. Activity with taurochenodeoxycholic acid is below detection limit | Lactiplantibacillus plantarum | ? | - |
? | |
| additional information | isozyme BSH4 activity compared to the other BSH isozymes from Lactobacillus plantarum strain GD2, substrate specificity, overview | Lactiplantibacillus plantarum | ? | - |
? | |
| additional information | isozyme BSH2 shows very low overall activity compared to the other BSH isozymes from Lactobacillus plantarum strain GD2. Substrate specificity, overview. Activity with taurodeoxycholic acid and taurochenodeoxycholic acid is below detection limit | Lactiplantibacillus plantarum GD2 | ? | - |
? | |
| additional information | isozyme BSH1 shows very high overall activity compared to the other BSH isozymes from Lactobacillus plantarum strain GD2. Substrate specificity, overview | Lactiplantibacillus plantarum GD2 | ? | - |
? | |
| additional information | isozyme BSH3 activity compared to the other BSH isozymes from Lactobacillus plantarum strain GD2, substrate specificity, overview. Activity with taurochenodeoxycholic acid is below detection limit | Lactiplantibacillus plantarum GD2 | ? | - |
? | |
| additional information | isozyme BSH4 activity compared to the other BSH isozymes from Lactobacillus plantarum strain GD2, substrate specificity, overview | Lactiplantibacillus plantarum GD2 | ? | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | chenodeoxycholate + taurine | - |
? | |
| taurochenodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum GD2 | chenodeoxycholate + taurine | - |
? | |
| taurocholic acid + H2O | - |
Lactiplantibacillus plantarum | cholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | deoxycholate + taurine | - |
? | |
| taurodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum GD2 | deoxycholate + taurine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35718, BSH3, sequence calculation | Lactiplantibacillus plantarum |
| ? | x * 36166, BSH4, sequence calculation | Lactiplantibacillus plantarum |
| ? | x * 36523, BSH2, sequence calculation | Lactiplantibacillus plantarum |
| ? | x * 37077, BSH1, sequence calculation | Lactiplantibacillus plantarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bile salt hydrolase | - |
Lactiplantibacillus plantarum |
| BSH | - |
Lactiplantibacillus plantarum |
| BSH1 | - |
Lactiplantibacillus plantarum |
| BSH2 | - |
Lactiplantibacillus plantarum |
| BSH3 | - |
Lactiplantibacillus plantarum |
| BSH4 | - |
Lactiplantibacillus plantarum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lactiplantibacillus plantarum | sequence calculation | - |
5.2 |
| Lactiplantibacillus plantarum | sequence calculation | - |
5.21 |
| Lactiplantibacillus plantarum | sequence calculation | - |
5.68 |
| Lactiplantibacillus plantarum | sequence calculation | - |
5.93 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the bile salt hydrolases and the penicillin V acylase family, phylogenetic analysis | Lactiplantibacillus plantarum |
| additional information | five putative active site residues, Cys2, Arg16, Asp19, Asn170, and Arg223 (bsh-1 enzyme amino acid numbering), are found in all four bsh proteins (BSH1-4) of Lactobacillus plantarum strain GD2, while the amino acid motifs around the active sites of BSH enzymes are not conserved among the four isozymes | Lactiplantibacillus plantarum |
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