Protein Variants | Comment | Organism |
---|---|---|
additional information | three twin-arginine signal peptides from twin-arginine translocation (Tat) pathway are synthesized, fused with bsh gene, inserted into expression vectors pET-20b(+) and pET-22b(+), and transformed into four different Escherichia coli hosts, respectively. Periplasmic export across the cytoplasmic membrane of bile salt hydrolase expressed in recombinant Escherichia coli by the twin-arginine signal peptides is achieved. Among the 24 recombinant bacteria obtained, Escherichia coli strain BL21(DE3) pLysS (pET-20b(+)-dmsA-bsh) shows the highest BSH activity in periplasmic fraction, which is further increased to 1.21 U/ml by orthogonal experimental design, overview. The presence of BSH in the periplasm is proven to be caused by the export rather than cell leakage. Export across the cytoplasmic membrane is initiated by the interaction of the signal peptides with the Tat export machinery, which in Escherichia coli is made up of the membrane protein TatABC | Lactiplantibacillus plantarum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | bile salt hydrolase, a hydrolytic and intracellular enzyme, can fold fully in the cytoplasm | Lactiplantibacillus plantarum | 5737 | - |
additional information | bile salt hydrolase from Lactobacillus plantarum BBE7 could not be efficiently exported by PelB signal peptide of the general secretory (Sec) pathway | Lactiplantibacillus plantarum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycodeoxycholic acid + H2O | Lactiplantibacillus plantarum | - |
deoxycholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | Lactiplantibacillus plantarum BBE7 | - |
deoxycholate + glycine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | B9V401 | - |
- |
Lactiplantibacillus plantarum BBE7 | B9V401 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | deoxycholate + glycine | - |
? | |
glycodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum BBE7 | deoxycholate + glycine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bile salt hydrolase | - |
Lactiplantibacillus plantarum |
BSH | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Lactiplantibacillus plantarum |
General Information | Comment | Organism |
---|---|---|
physiological function | bile salt hydrolase catalyzes the hydrolysis of glycine- or taurine-conjugated bile acids into the free bile acids and a glycine/taurine moiety, which will increase the de novo synthesis of bile acids from cholesterol in the human host, thus lowering the host serum cholesterol level | Lactiplantibacillus plantarum |