Application | Comment | Organism |
---|---|---|
nutrition | BSH is a key mechanistic microbiome target for the development of non-antibiotic food additives to improve animal feed production and for the design of measures to control obesity in humans | Ligilactobacillus salivarius |
Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His-tagged BSH in Escherichia coli strain BL21(DE3) | Ligilactobacillus salivarius |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, mixing 200 nl of 16.0 mg/ml protein in 10 mM sodium acetate, pH 5.5, 400 mM NaCl, 1 mM DTT, 1 mM EDTA, and 10% glycerol with 200 nl of reservoir solution containing 20% PEG 3350, and 0.2 M potassium dihydrogen phosphate, pH 4.8, and equilibration against 0.15 ml reservoir solution, X-ray diffraction structure determination and analysis at 1.90 A resolution, molecular replacement using the structure of Clostridium perfringens BSH as a starting model. Two BSH molecules are packed perfectly as a dimer in one asymmetric unit | Ligilactobacillus salivarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Ligilactobacillus salivarius | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ligilactobacillus salivarius | J7GY78 | - |
- |
Ligilactobacillus salivarius NRRL B-30514 | J7GY78 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged BSH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange and hydrophobic interaction chromatography, and gel filtration | Ligilactobacillus salivarius |
Source Tissue | Comment | Organism | Textmining |
---|
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Ligilactobacillus salivarius |
Synonyms | Comment | Organism |
---|---|---|
bile salt hydrolase | - |
Ligilactobacillus salivarius |
BSH | - |
Ligilactobacillus salivarius |
lsBSH | - |
Ligilactobacillus salivarius |
General Information | Comment | Organism |
---|---|---|
evolution | bile salt hydrolase (BSH) is a member of the N-terminal nucleophile hydrolase superfamily. Structure comparisons | Ligilactobacillus salivarius |
additional information | identification of residues involved in catalysis and substrate specificity, structure-function analysis of BSH | Ligilactobacillus salivarius |
physiological function | bile salt hydrolase (BSH) is a gut-bacterial enzyme that negatively influences host fat digestion and energy harvesting. The BSH enzyme activity functions as a gateway reaction in the small intestine by the deconjugation of glycineconjugated or taurine-conjugated bile acids. BSH enzyme catalyzes the deconjugation of glycine-conjugated or taurine-conjugated bile acids, which is an essential gateway reaction in the metabolism of bile acids in the small intestine | Ligilactobacillus salivarius |