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Literature summary for 3.5.1.24 extracted from
- Secretory expression and characterization of a bile salt hydrolase from Lactobacillus plantarum in Escherichia coli (2013), J. Mol. Catal. B, 93, 57-64.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | - |
Lactiplantibacillus plantarum |  |
| EDTA | - |
Lactiplantibacillus plantarum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS and usage of the twin-arginine translocation (Tat) pathway as an alternative for this secretory production, enzyme secretion by the twin-arginine signal peptide of DMSO reductase subunit DmsA from Escherichia coli, method, overview. Most of the enzyme is expresed in intracellular inclusion bodies, but some is secreted to the cell culture medium | Lactiplantibacillus plantarum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 36% inhibition at 2 mM | Lactiplantibacillus plantarum | |
| CuCl2 | complete inhibition at 2 mM | Lactiplantibacillus plantarum | |
| FeCl3 | 42% inhibition at 2 mM, 95.6% at 10 mM | Lactiplantibacillus plantarum | |
| MgCl2 | 48% inhibition at 2 mM | Lactiplantibacillus plantarum | |
| MnCl2 | 75% inhibition at 0.1 mM, 97% at 10 mM | Lactiplantibacillus plantarum | |
| PMSF | 28% inhibition at 2 mM, 67% at 10 mM | Lactiplantibacillus plantarum | |
| Sodium periodate | about 99% inhibition at 0.1-2 mM, complete inhibition at 10 mM | Lactiplantibacillus plantarum | |
| ZnSO4 | 53% inhibition at 0.1 mM, 89% at 2 mM, 98% at 10 mM | Lactiplantibacillus plantarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.07 | - |
glycodeoxycholic acid | pH 6.0, 37°C, recombinant enzyme | Lactiplantibacillus plantarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Lactiplantibacillus plantarum |
| 140000 | 150000 | recombinant His-tagged enzyme, gel filtration | Lactiplantibacillus plantarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum | - |
- |
- |
| Lactiplantibacillus plantarum BBE7 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble His-tagged enzyme 39.34fold from Escherichia coli strain BL21(DE3) pLysS by ammonium sulfate fractionation, nickel affinity chromatography, and gel filtration, to homogeneity | Lactiplantibacillus plantarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 274.2 | - |
pH 6.0, 37°C, recombinant enzyme | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | deoxycholate + glycine | - |
? | |
| glycodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum BBE7 | deoxycholate + glycine | - |
? | |
| additional information | the recombinant enzyme is more efficient in hydrolyzing glycoconjugated bile salts than tauroconjugated bile salts | Lactiplantibacillus plantarum | ? | - |
? | |
| additional information | the recombinant enzyme is more efficient in hydrolyzing glycoconjugated bile salts than tauroconjugated bile salts | Lactiplantibacillus plantarum BBE7 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Lactiplantibacillus plantarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bile salt hydrolase | - |
Lactiplantibacillus plantarum |
| BSH | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
recombinant His-tagged enzyme | Lactiplantibacillus plantarum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
50 | activity range, temperature profile overview | Lactiplantibacillus plantarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant His-tagged enzyme, pH 6.0, 60 min, 90% activity remaining | Lactiplantibacillus plantarum |
| 50 | - |
purified recombinant His-tagged enzyme, pH 6.0, 60 min, 20-30% activity remaining | Lactiplantibacillus plantarum |
| 60 | - |
purified recombinant His-tagged enzyme, pH 6.0, inactivation within 10 min | Lactiplantibacillus plantarum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 88.13 | - |
glycodeoxycholic acid | pH 6.0, 37°C, recombinant enzyme | Lactiplantibacillus plantarum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
recombinant His-tagged enzyme | Lactiplantibacillus plantarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 7.3 | activity range, pH profile overview | Lactiplantibacillus plantarum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
purified recombinant His-tagged enzyme, 37°C, 24 h, inactivation | Lactiplantibacillus plantarum |
| 5 | - |
purified recombinant His-tagged enzyme, 37°C, 24 h, 50% activity remaining | Lactiplantibacillus plantarum |
| 7 | 8 | purified recombinant His-tagged enzyme, 37°C, 24 h, stable at | Lactiplantibacillus plantarum |
| 9 | - |
purified recombinant His-tagged enzyme, 37°C, 24 h, 50% activity remaining | Lactiplantibacillus plantarum |
| 10 | - |
purified recombinant His-tagged enzyme, 37°C, 24 h, inactivation | Lactiplantibacillus plantarum |
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