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Literature summary for 3.5.1.24 extracted from

  • Sridevi, N.; Srivastava, S.; Khan, B.M.; Prabhune, A.A.
    Characterization of the smallest dimeric bile salt hydrolase from a thermophile Brevibacillus sp (2009), Extremophiles, 13, 363-370.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
FeSO4 114.7% residual activity Brevibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-2-nitrobenzoic acid 65% residual activity Brevibacillus sp.
AgNO3 77.3% residual activity Brevibacillus sp.
BaCl2 78.4% residual activity Brevibacillus sp.
citraconic anhydride 33.3% residual activity Brevibacillus sp.
CoCl2 90.9% residual activity Brevibacillus sp.
CuSO4 0% residual activity Brevibacillus sp.
EDTA 80.69% residual activity Brevibacillus sp.
HgCl2 43.2% residual activity Brevibacillus sp.
MgSO4 87.5% residual activity Brevibacillus sp.
MnSO4 95.5% residual activity Brevibacillus sp.
N-bromosuccinimide 63.6% residual activity Brevibacillus sp.
NiSO4 92.0% residual activity Brevibacillus sp.
Phenylglyoxal 57.4% residual activity Brevibacillus sp.
Trinitrobenzenesulfonic acid 30.9% residual activity Brevibacillus sp.
Woodward's reagent K 53.6% residual activity Brevibacillus sp.
ZnSO4 76.1% residual activity Brevibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00308
-
glycodeoxycholic acid apparent value, calculated from a Lineweaver-Burk plot Brevibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
monomer, determined by SDS-PAGE Brevibacillus sp.
28200
-
monomer, determined by MALDI-TOF analysis Brevibacillus sp.
56000
-
native protein, determined by gel filtration Brevibacillus sp.

Organism

Organism UniProt Comment Textmining
Brevibacillus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity using Q-Sepharose chromatography Brevibacillus sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.03
-
purification step sonification Brevibacillus sp.
12.2
-
purification step first Q-Sepharose chromatograpgy Brevibacillus sp.
13.8
-
purification step second Q-Sepharose chromatograpgy Brevibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycochenodeoxycholic acid + H2O
-
Brevibacillus sp. 3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
-
?
glycocholic acid + H2O
-
Brevibacillus sp. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
-
?
glycodeoxycholic acid + H2O
-
Brevibacillus sp. 3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
?
taurochenodeoxycholic acid + H2O
-
Brevibacillus sp. chenodeoxycholate + taurine
-
?
taurocholic acid + H2O
-
Brevibacillus sp. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
?
taurodeoxycholic acid + H2O
-
Brevibacillus sp. 3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
?

Subunits

Subunits Comment Organism
homodimer
-
Brevibacillus sp.

Synonyms

Synonyms Comment Organism
bile salt hydrolase
-
Brevibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
activity assay Brevibacillus sp.
60
-
-
Brevibacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
632
-
glycodeoxycholic acid apparent value, calculated from a Lineweaver-Burk plot Brevibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
activity assay Brevibacillus sp.
9
-
-
Brevibacillus sp.