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Literature summary for 3.5.1.23 extracted from
- Purification and biochemical characterization of membrane-bound neutral ceramidase from camel brain (Camelus dromedarius) (2013), Int. J. Biochem. Mol. Biol., 4, 54-66.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Camelus dromedarius |  |
| Cu2+ | - |
Camelus dromedarius | |
| cysteine | - |
Camelus dromedarius | |
| GSH | - |
Camelus dromedarius | |
| GSSG | - |
Camelus dromedarius | |
| N-acetylcysteine | - |
Camelus dromedarius | |
| phosphatidic acid | complete inhibition | Camelus dromedarius | |
| phosphatidylcholine | complete inhibition | Camelus dromedarius | |
| phosphatidylserine | complete inhibition | Camelus dromedarius | |
| TMP | - |
Camelus dromedarius | |
| UMP | - |
Camelus dromedarius | |
| Zn2+ | - |
Camelus dromedarius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Camelus dromedarius | |
| 0.0498 | - |
C12-NBD-Cer | pH 7.5, 37°C | Camelus dromedarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Camelus dromedarius | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Camelus dromedarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE | Camelus dromedarius |
| 100000 | - |
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE | Camelus dromedarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelus dromedarius | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | deeglycosylation by glycosidase F treatment | Camelus dromedarius |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 2147.1fold from brain to homogeneity by anion exchange and hydrophobic interaction chromtography, gel filtration, and another step of anion exchange chromatography | Camelus dromedarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Camelus dromedarius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 230.6 | - |
purified enzyme, pH 7.5, 37°C | Camelus dromedarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| C12-NBD-Cer + H2O | the enzyme catalyzes also the reverse reaction of ceramide synthesis | Camelus dromedarius | NBD-dodecanoic acid + D-erythro-sphingosine | - |
r | |
| N-hexanoyl-D-erythro-sphingosine + H2O | - |
Camelus dromedarius | hexanoate + D-erythro-sphingosine | - |
? | |
| N-lauroyl-D-erythro-sphingosine + H2O | the enzyme catalyzes also the reverse reaction of ceramide synthesis | Camelus dromedarius | laurate + D-erythro-sphingosine | - |
r | |
| N-lignoceroyl-D-erythro-sphingosine + H2O | low activity | Camelus dromedarius | lignocerate + D-erythro-sphingosine | - |
? | |
| N-myristoyl-D-erythro-sphingosine + H2O | best substrate | Camelus dromedarius | myristate + D-erythro-sphingosine | - |
? | |
| N-palmitoyl-D-erythro-sphingosine + H2O | - |
Camelus dromedarius | palmitate + D-erythro-sphingosine | - |
? | |
| N-stearoyl-D-erythro-sphingosine + H2O | - |
Camelus dromedarius | stearate + D-erythro-sphingosine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE | Camelus dromedarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBCDase | - |
Camelus dromedarius |
| nCDase | - |
Camelus dromedarius |
| neutral ceramidase | - |
Camelus dromedarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Camelus dromedarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Camelus dromedarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | activity range, high activity at pH 6.0-8.0, profile overview | Camelus dromedarius |
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