Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | ACTH/cAMP signaling stimulates the enzyme transcription and activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene ASAH1, expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a tetracycline-inducible ASAH1 short hairpin RNA H295R human adrenocortical stable cell line | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ceramide + H2O | Homo sapiens | - |
sphingosine + fatty acid | - |
? | |
ceramide + H2O | Homo sapiens | - |
carboxylate + sphingosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q13510 | gene ASAH1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Homo sapiens | - |
adrenocortical carcinoma cell line | - |
Homo sapiens | - |
NCI-H295R cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ceramide + H2O | - |
Homo sapiens | sphingosine + fatty acid | - |
? | |
ceramide + H2O | - |
Homo sapiens | carboxylate + sphingosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid ceramidase | - |
Homo sapiens |
ASAH1 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | ACTH/cAMP signaling stimulates acid ceramidase ASAH1 transcription and activity | up |
Homo sapiens | ACTH/cAMP signaling stimulates the enzyme transcription and activity | up |
General Information | Comment | Organism |
---|---|---|
malfunction | acid ceramidase ASAH1 knockdown leads to a decrease in cell proliferation with a concomitant reduction in the protein levels of BETA-catenin, proliferating cell nuclear antigen, and cyclin B2. ASAH1 silencing increases basal and cAMP-dependent cortisol and dehydroepiandrosterone secretion | Homo sapiens |
malfunction | a tetracycline-inducible ASAH1 short hairpin RNA H295R human adrenocortical stable cell line shows increased transcription of multiple steroidogenic genes, including Cytochrome P450 monooxygenase (CYP)17A1, CYP11B1/2, CYP21A2, steroidogenic acute regulatory protein, hormone-sensitive lipase, 18-kDa translocator protein, and the melanocortin-2 receptor. Induced gene expression positively correlates with enhanced histone H3 acetylation at target promoters. Repression of ASAH1 expression also induces the expression of members of the nuclear receptor nuclear receptor subfamily 4 family while concomitantly suppressing the expression of dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1. ASAH1 knockdown alters the expression of genes involved in sphingolipid metabolism and changes the cellular amounts of distinct sphingolipid species. Enzyme silencing increases basal and cAMP-dependent cortisol and dehydroepiandrosterone secretion, establishing ASAH1 as a pivotal regulator of steroidogenic capacity in the human adrenal cortex | Homo sapiens |
physiological function | acid ceramidase ASAH1 is a global regulator of steroidogenic capacity and adrenocortical gene expression | Homo sapiens |
physiological function | acid ceramidase directly regulates the intracellular balance of ceramide, sphingosine, and sphingosine-1-phosphate by catalyzing the hydrolysis of ceramide into sphingosine. The enzyme plays a role in glucocorticoid production and regulating steroidogenic capacity | Homo sapiens |