Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cardiolipin | - |
Aspergillus oryzae RIB40 | |
additional information | phosphatidylethanolamine and phosphatidylcholine have little effect on the activity regardless of the concentration | Aspergillus oryzae RIB40 | |
phosphatidylglycerol | - |
Aspergillus oryzae RIB40 | |
phosphatidylserine | - |
Aspergillus oryzae RIB40 |
Cloned (Comment) | Organism |
---|---|
gene asahA, DNA and amino acid sequence determination and analysis, overexpression of HA- and His6-tagged neutral CDase in Aspergillus oryzae | Aspergillus oryzae RIB40 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 15% inhibition at 5 mM | Aspergillus oryzae RIB40 | |
Hg2+ | 50% inhibition at 5 mM | Aspergillus oryzae RIB40 | |
Mg2+ | 10% inhibition at 5 mM | Aspergillus oryzae RIB40 | |
Mn2+ | 25-30% inhibition at 5 mM | Aspergillus oryzae RIB40 | |
phosphate | 80% inhibition at 100 mM | Aspergillus oryzae RIB40 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Aspergillus oryzae RIB40 | |
0.00332 | - |
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl]-sphingosine | pH 4.0, 40°C | Aspergillus oryzae RIB40 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activate about 18% at 5 mM | Aspergillus oryzae RIB40 | |
additional information | Zn2+, and EDTA have little effect on the activity | Aspergillus oryzae RIB40 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
x * 97000, recombinant enzyme, SDS-PAGE | Aspergillus oryzae RIB40 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae RIB40 | Q2UN98 | gene asahA | - |
Purification (Comment) | Organism |
---|---|
recombinant HA- and His6-tagged AsahA 90fold from Aspergillus oryzae | Aspergillus oryzae RIB40 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
C24-ceramide + H2O | - |
Aspergillus oryzae RIB40 | C24 fatty acid + sphingosine | - |
r | |
hexadecanoyl-ceramide + H2O | i.e. C16-ceramide | Aspergillus oryzae RIB40 | hexadecanoate + sphingosine | - |
r | |
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl]-sphingosine + H2O | i.e. C12-NBD-ceramide | Aspergillus oryzae RIB40 | N-12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]laurate + sphingosine | - |
r | |
octadecanoyl-ceramide + H2O | i.e. C18-ceramide | Aspergillus oryzae RIB40 | octadecanoate + sphingosine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 97000, recombinant enzyme, SDS-PAGE | Aspergillus oryzae RIB40 |
Synonyms | Comment | Organism |
---|---|---|
AsahA | - |
Aspergillus oryzae RIB40 |
CDase | - |
Aspergillus oryzae RIB40 |
neutral CDase | - |
Aspergillus oryzae RIB40 |
neutral ceramidase | - |
Aspergillus oryzae RIB40 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
hydrolysis and synthesis reactions | Aspergillus oryzae RIB40 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
hydrolysis reaction | Aspergillus oryzae RIB40 |
5 | - |
synthesis reaction, with NBD-dodecanoic acid and sphingosine as substrates | Aspergillus oryzae RIB40 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | activity range of neutral CDase, profile overview | Aspergillus oryzae RIB40 |