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Literature summary for 3.5.1.23 extracted from
- Expression, purification, and characterization of a recombinant neutral ceramidase from Mycobacterium tuberculosis (2010), Biosci. Biotechnol. Biochem., 74, 316-321.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Mycobacterium tuberculosis |  |
| EGTA | - |
Mycobacterium tuberculosis | |
| sodium taurodeoxycholate | activates the recombinant enzyme about 2fold, optimally at 1% | Mycobacterium tuberculosis | |
| Triton X-100 | activates the recombinant enzyme about 2fold, optimally at 0.05% | Mycobacterium tuberculosis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged CDase in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Mycobacterium tuberculosis | |
| Co2+ | - |
Mycobacterium tuberculosis | |
| Cu2+ | - |
Mycobacterium tuberculosis | |
| Mg2+ | - |
Mycobacterium tuberculosis | |
| Mn2+ | - |
Mycobacterium tuberculosis | |
| Ni2+ | - |
Mycobacterium tuberculosis | |
| Zn2+ | - |
Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics of hydrolysis, overview | Mycobacterium tuberculosis | |
| 0.0987 | - |
N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-sphingosine | pH 8.0, 37°C | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 71000 | - |
x * 71000, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ceramide + H2O | Mycobacterium tuberculosis | - |
sphingosine + fatty acid | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged CDase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ceramide + H2O | - |
Mycobacterium tuberculosis | sphingosine + fatty acid | - |
r | |
| ceramide + H2O | the recombinant enzyme shows a broad subsrate specificity with ceramides, it shows good activity with ceramides containing C6-C24 fatty acids, and preference of ceramides with monounsaturated fatty acids than saturated fatty acids, overview | Mycobacterium tuberculosis | sphingosine + fatty acid | - |
r | |
| N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-dihydrosphingosine + H2O | - |
Mycobacterium tuberculosis | 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoate + D-erythro-dihydrosphingosine | - |
r | |
| N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-phytosphingosine + H2O | - |
Mycobacterium tuberculosis | 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-phytosphingosine | - |
r | |
| N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-sphingosine + H2O | i.e. C12-NBD-Cer | Mycobacterium tuberculosis | 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-sphingosine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 71000, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDase | - |
Mycobacterium tuberculosis |
| neutral ceramidase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | recombinant enzyme, forward reaction | Mycobacterium tuberculosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | activity range, recombinant enzyme, forward reaction, profile overview | Mycobacterium tuberculosis |
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