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Literature summary for 3.5.1.23 extracted from
- Ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa (2007), J. Biol. Chem., 282, 6021-6030.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is induced by lipids | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cdase, the gene encoding the enzyme is adjacent to that encoding hemolytic phospholipase C, plcH, in the genome of Pseudomonas aeruginosa | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a CDase-null mutant via homologous recombination and gene disruption, CDase-deficient mutants lack any CDase activity, the hemolytic activity by the CDase mutants against human erythrocytes is significantly reduced compared with that by the wild-type | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Pseudomonas aeruginosa |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas aeruginosa | ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa | ? | - |
? | |
| additional information | Pseudomonas aeruginosa | ceramidase enhances cytotoxicity induced by hemolytic phospholipase C | ? | - |
? | |
| additional information | Pseudomonas aeruginosa AN17 | ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa | ? | - |
? | |
| additional information | Pseudomonas aeruginosa AN17 | ceramidase enhances cytotoxicity induced by hemolytic phospholipase C | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa | - |
gene cdase or PA0845 | - |
| Pseudomonas aeruginosa AN17 | - |
gene cdase or PA0845 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition | when the strain is cultured with sphingomyelin or phosphatidylcholine, production of the enzyme drastically increases, causing the increase of hemolytic activity in the cellfree culture supernatant. Ceramide and sphingosine are effective in promoting the production of ceramidase | Pseudomonas aeruginosa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O | C12 substrate chain length | Pseudomonas aeruginosa | ? | - |
? | |
| 4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O | C12 substrate chain length | Pseudomonas aeruginosa AN17 | ? | - |
? | |
| additional information | ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | ceramidase enhances cytotoxicity induced by hemolytic phospholipase C | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa | Pseudomonas aeruginosa AN17 | ? | - |
? | |
| additional information | ceramidase enhances cytotoxicity induced by hemolytic phospholipase C | Pseudomonas aeruginosa AN17 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDase | - |
Pseudomonas aeruginosa |
| neutral ceramidase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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