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Literature summary for 3.5.1.23 extracted from
- Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P (2006), FASEB J., 20, 1813-1825.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | serum deprivation up-regulates both haCER2 mRNA and activity in recombinant HeLa cells, haCER2mRNA is also up-regulated in some tumors | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the gene encoding CER2 is located on chromosome 9, cloning from a liver cDNA library, functional expression of CER2 in HeLa cells showing 20fold increased enzyme activity in microsomes | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | high ectopic expression of haCER2 causes fragmentation of the Golgi complex and growth arrest in HeLa cells due to sphingosine accumulation, low ectopic expression increases sphingosine 1-phosphate level without sphingosine accumulation, promoting cell proliferation in serum-free medium, this proliferative effect is suppressed by dimethylsphingosine, an inhibitor of the S1P formation, or by RNAi -mediated inhibition of S1P1, a G-protein-coupled receptor for S1P, the RNAi-mediated down-regulation of haCER2 enhances the serum deprivation-induced growth arrest and apoptosis of HeLa cells, which is inhibited by addition of exogenous S1P, serum deprivation up-regulates both haCER2 mRNA and activity in recombinant HeLa cells, haCER2mRNA is also up-regulated in some tumors, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | CER2 | Homo sapiens | 5794 | - |
| membrane | transmembrane protein | Homo sapiens | 16020 | - |
| microsome | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythro-myristoyl-sphingosine + H2O | Homo sapiens | - |
myristate + sphingosine | - |
? |  |
| D-erythro-oleoyl-sphingosine + H2O | Homo sapiens | - |
oleate + sphingosine | - |
? | |
| D-erythro-palmitoyl-sphingosine + H2O | Homo sapiens | - |
palmitate + sphingosine | - |
? | |
| D-erythro-tetracosanoyl-sphingosine + H2O | Homo sapiens | i.e. D-e-C24:0-ceramide | tetracosanoate + sphingosine | - |
? | |
| additional information | Homo sapiens | CER2 catalyzes the hydrolysis of ceramides derived from both the de novo and sphingomyelin breakdown pathways, Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and sphingosine 1-phosphate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q5QJU3 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
| additional information | tissue expression analysis | Homo sapiens | - |
| placenta | high expression level of CER2 | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
sphingolipid level analysis by mass spectrometry, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythro-myristoyl-sphingosine + H2O | - |
Homo sapiens | myristate + sphingosine | - |
? | |
| D-erythro-oleoyl-sphingosine + H2O | - |
Homo sapiens | oleate + sphingosine | - |
? | |
| D-erythro-palmitoyl-sphingosine + H2O | - |
Homo sapiens | palmitate + sphingosine | - |
? | |
| D-erythro-tetracosanoyl-sphingosine + H2O | i.e. D-e-C24:0-ceramide | Homo sapiens | tetracosanoate + sphingosine | - |
? | |
| additional information | CER2 catalyzes the hydrolysis of ceramides derived from both the de novo and sphingomyelin breakdown pathways, Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and sphingosine 1-phosphate | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CER2 | - |
Homo sapiens |
| Golgi alkaline ceramidase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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