Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae enzyme-deficient doubleknockout strain.ypc1.ydc1 microsomes under control of the Gal1 promoter | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C362A | site-directed mutagenesis, inactive enzyme | Homo sapiens |
D352A | site-directed mutagenesis, inactive enzyme | Homo sapiens |
S258A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Homo sapiens |
S354A | site-directed mutagenesis, inactive enzyme | Homo sapiens |
S374A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
S396A | site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
S595A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
S729A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Homo sapiens | |
2-mercaptoethanol | - |
Rattus norvegicus | |
Cu2+ | - |
Homo sapiens | |
Cu2+ | - |
Rattus norvegicus | |
di-isopropyl fluorophosphate | the substrate protects against inhibition | Homo sapiens | |
di-isopropyl fluorophosphate | the substrate protects against inhibition | Rattus norvegicus | |
dithiothreitol | - |
Homo sapiens | |
dithiothreitol | - |
Rattus norvegicus | |
Fe2+ | - |
Homo sapiens | |
Fe2+ | - |
Rattus norvegicus | |
additional information | no inhibition by protease inhibitors such as aprotinin, PMSF, leupeptin, and pepstatin | Homo sapiens | |
additional information | no inhibition by protease inhibitors such as aprotinin, PMSF, leupeptin, and pepstatin | Rattus norvegicus | |
Zn2+ | - |
Homo sapiens | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | classical MichaelisMenten kinetics | Homo sapiens | |
0.06 | - |
D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide | pH 7.5, 37°C, recombinant enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Rattus norvegicus | |
Ca2+ | activates | Homo sapiens | |
Mg2+ | activates slightly | Rattus norvegicus | |
Mg2+ | activates slightly | Homo sapiens | |
Mn2+ | activates slightly | Rattus norvegicus | |
Mn2+ | activates slightly | Homo sapiens | |
Na+ | activates | Rattus norvegicus | |
Na+ | activates | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NR71 | - |
- |
no activity in Saccharomyces cerevisiae | - |
- |
- |
Rattus norvegicus | Q91XT9 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 20000fold from brain by anion exchange chromatography, affinity and hydrophobic interaction chromatography, and cation exchange chromatography, to homogeneity | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a ceramide + H2O = a carboxylate + sphingosine | catalytic mechanism | Rattus norvegicus | |
a ceramide + H2O = a carboxylate + sphingosine | the enzyme has a catalytic residue Ser354 within a very highly conserved hexapeptide sequence GDVSPN in the middle of a larger conserved sequence, namely NXGDVSPNXXGP/XXC, catalytic mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activities of wild-type and mutant enzymes, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O | - |
Homo sapiens | 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide | - |
? | |
D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O | - |
Rattus norvegicus | D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide | - |
? | |
D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O | - |
Homo sapiens | D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide | - |
? | |
D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O | - |
Homo sapiens | D-erythro-sphingosine + ? | - |
? | |
palmitoyl-sphingosine + H2O | - |
Rattus norvegicus | palmitate + sphingosine | - |
r | |
palmitoyl-sphingosine + H2O | - |
Homo sapiens | palmitate + sphingosine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CDase | - |
Rattus norvegicus |
CDase | - |
Homo sapiens |
neutral ceramidase | - |
Rattus norvegicus |
neutral ceramidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Homo sapiens |
7.5 | - |
assay at | Rattus norvegicus |