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Literature summary for 3.5.1.23 extracted from

  • El Bawab, S.; Birbes, H.; Roddy, P.; Szulc, Z.M.; Bielawska, A.; Hannun, Y.A.
    Biochemical characterization of the reverse activity of rat brain ceramidase. A CoA-independent and fumonisin B1-insensitive ceramide synthase (2001), J. Biol. Chem., 276, 16758-16766.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin activates the ceramidase activity by 2.5fold at 8-10 mol%, inhibits the ceramide synthesis activity, mechanism Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol inhibits both ceramidase and ceramide synthesis activity Rattus norvegicus
C16-ceramide ceramide synthesis activity, 10 mol%, 50% inhibition Rattus norvegicus
cardiolipin inhibits the ceramide synthesis activity: total inhibition at 2.5-5 mol%, activates the ceramidase activity, mechanism Rattus norvegicus
CuCl2 ceramide synthesis activity, 1 mM, total inhibition Rattus norvegicus
dithiothreitol inhibits both ceramidase and ceramide synthesis activity Rattus norvegicus
L-erythro-sphingosine competitive inhibitor of ceramide synthesis activity Rattus norvegicus
lysophosphatidylcholine ceramide synthesis activity, 10 mol%, moderate inhibition Rattus norvegicus
additional information the ceramide synthesis activity is not inhibited in vitro and in cells by fuminosin B1, not inhibited by EDTA or ATP, both up to 10 mM Rattus norvegicus
myristaldehyde competitively inhibits the ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition Rattus norvegicus
palmitaldehyde ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition Rattus norvegicus
phosphatidic acid ceramide synthesis activity, 2.5-5 mol%, total inhibition Rattus norvegicus
phosphatidylcholine ceramide synthesis activity, 10 mol%, moderate inhibition Rattus norvegicus
phosphatidylglycerol ceramide synthesis activity, 10 mol%, moderate inhibition Rattus norvegicus
phosphatidylserine ceramide synthesis activity, 10 mol%, moderate inhibition Rattus norvegicus
sphingomyelin ceramide synthesis activity, 5 mol%, 50% inhibition Rattus norvegicus
ZnCl2 ceramide synthesis activity, 1 mM, total inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic data for the ceramide synthesis reaction, random sequential kinetic mechanism Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound, brain Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the ceramide synthesis activity is totally independent of stimulating cations Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
a ceramide + H2O = a carboxylate + sphingosine kinetic mechanism Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-erythro-dihydrosphingosine + fatty acid very low ceramide synthesis activity Rattus norvegicus ?
-
?
D-erythro-sphingosine + fatty acid highest ceramide synthesis activity with D-erythro-sphingosine Rattus norvegicus ?
-
?
D-threo-sphingosine + fatty acid low ceramide synthesis activity Rattus norvegicus ?
-
?
L-erythro-sphingosine + fatty acid low ceramide synthesis activity Rattus norvegicus ?
-
?
L-threo-sphingosine + fatty acid low ceramide synthesis activity Rattus norvegicus ?
-
?
additional information no substrate in the ceramide synthesis reaction: palmitoyl-CoA Rattus norvegicus ?
-
?
myristic acid + sphingosine highest ceramide synthesis activity with myristic acid Rattus norvegicus N-myristoylsphingosine + H2O
-
?
N-acylsphingosine + H2O CDase cleaves the N-acyl linkage of ceramide into sphingosine and free fatty acid, brain CDase is able to catalyze the reverse reaction, i.e. ceramide synthesis Rattus norvegicus a carboxylate + sphingosine
-
r
N-palmitoylsphingosine + H2O
-
Rattus norvegicus palmitic acid + sphingosine
-
r

Synonyms

Synonyms Comment Organism
CDase
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 ceramide synthesis activity, narrow optimum in the neutral range Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 very low ceramide synthesis activity below pH 5 or above pH 8 Rattus norvegicus
5.5 10 broad pH-range, ceramidase activity Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
additional information coenzyme A-independent mechanism Rattus norvegicus