Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ceramide-1-phosphate | stimulates | Rattus norvegicus | |
sphingosine-1-phosphate | stimulates | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2S)-3-keto-dehydrosphingosine | mtCDase, weak inhibition | Rattus norvegicus | |
(2S)-3-keto-hexadecanoyl-ceramide | mtCDase, IC50: 0.6 mol% | Rattus norvegicus | |
(2S)-3-keto-sphinganine | mtCDase, IC50: 0.34 mol% | Rattus norvegicus | |
1-O-methyl-D-erythro-sphingosine | mtCDase, weak inhibition | Rattus norvegicus | |
C18-ceramide | mtCDase, competitive inhibition, IC50: 0.62 mol% | Rattus norvegicus | |
cis-D-erythro-sphingosine | mtCDase, weak inhibition | Rattus norvegicus | |
D-erythro-dehydrosphingosine | mtCDase, IC50: 0.25 mol% | Rattus norvegicus | |
D-erythro-sphinganine | mtCDase, IC50: 0.2 mol% | Rattus norvegicus | |
D-erythro-sphingosine | IC50: 0.04 mol%, mt-CDase is inhibited by all stereoisomers of sphingosine with IC50 ranging from 0.04 to 0.14 mol% | Rattus norvegicus | |
D-erythro-urea-C16-ceramide | mtCDase, competitive inhibition, IC50: 0.33 mol% | Rattus norvegicus | |
D-threo-ceramide | mt-CDase, IC50: 0.21 mol% | Rattus norvegicus | |
L-erythro-ceramide | mt-CDase, IC50: 0.26 mol% | Rattus norvegicus | |
L-threo-ceramide | mt-CDase, IC50: 0.11 mol% | Rattus norvegicus | |
additional information | not inhibited by N-methyl-hexadecanoyl-ceramide, 1-O-methyl-hexadecanoyl-ceramide, 3-O-methyl-hexadecanoyl-ceramide, cis-D-erythro-hexadecanoyl-ceramide, 3-O-methyl-D-erythro-sphingosine, requirements for inhibition of mtCDase | Rattus norvegicus | |
N,N-dimethyl-D-erythro-sphingosine | mtCDase, weak inhibition | Rattus norvegicus | |
N-methyl-D-erythro-sphingosine | mtCDase, IC50: 0.13 mol% | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythro-octadecanoyl-ceramide + H2O | mtCDase specifically hydrolyzes the D-erythro-isomer of ceramide, requirements for ceramide-enzyme interaction, ligand interaction with the enzyme occurs in a high affinity low specificity manner, in contrast to catalysis which is highly specific for D-erythro-ceramide but occurs with a lower affinity | Rattus norvegicus | D-erythro-sphingosine + palmitic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mt-CDase | mitochondrial ceramidase | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
assay at | Rattus norvegicus |