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Literature summary for 3.5.1.23 extracted from

  • Gatt, S.
    Enzymatic hydrolysis of sphingolipids. I. Hydrolysis and synthesis of ceramides by an enzyme from rat brain (1966), J. Biol. Chem., 241, 3724-3730.
    View publication on PubMed
Search for more literature for 3.5.1.23

Activating Compound

Activating Compound Comment Organism Structure
sodium cholate
-
 Rattus norvegicus
Triton X-100
-
 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
fatty acids
-
 Rattus norvegicus
laureate at pH 5 Rattus norvegicus
palmitate at pH 5 Rattus norvegicus
sphingosine
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 sphingosine
-
 Rattus norvegicus
0.3
-
 ceramide
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
100fold Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-20°C, 1 year, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ceramide + H2O
-
 Rattus norvegicus sphingosine + fatty acid
-
 r
N-oleyldihydro-sphingosine + H2O
-
 Rattus norvegicus ?
-
 r
N-palmitoyldihydrosphingosine + H2O
-
 Rattus norvegicus ?
-
 r
N-palmitoylsphingosine + H2O
-
 Rattus norvegicus palmitic acid + sphingosine
-
 r
N-stearoylsphingosine + H2O
-
 Rattus norvegicus stearic acid + sphingosine
-
 r
sphingosine + fatty acid
-
 Rattus norvegicus ceramide
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Rattus norvegicus