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Literature summary for 3.5.1.20 extracted from

  • Zhu, T.; Fang, S.; Wang, W.; Wang, K.; Cui, Z.; Wang, C.
    The expression, purification and activity analysis of Francisella tularensis citrulline ureidase in Escherichia coli (2015), J. Appl. Biomed., 13, 189-194 .
No PubMed abstract available
Search for more literature for 3.5.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The soluble and active recombinant enzyme is expressed in the periplasmic space with the vector pET-22b Francisella tularensis subsp. tularensis

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Francisella tularensis subsp. tularensis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 SDS-PAGE Francisella tularensis subsp. tularensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-citrulline + H2O Francisella tularensis subsp. tularensis
-
 L-ornithine + CO2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Francisella tularensis subsp. tularensis Q5NHL7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme Francisella tularensis subsp. tularensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20 24 30°C, pH 6.5 Francisella tularensis subsp. tularensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-citrulline + H2O
-
 Francisella tularensis subsp. tularensis L-ornithine + CO2 + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Francisella tularensis subsp. tularensis

Synonyms

Synonyms Comment Organism
citrulline ureidase
-
 Francisella tularensis subsp. tularensis
CTU
-
 Francisella tularensis subsp. tularensis