Literature summary for 3.5.1.2 extracted from

Shah, L.; Nadeem, M.; Khan, J.; Zeyadi, M.; Zamzami, M.; Mohammed, K.
Recombinant L-glutaminase obtained from Geobacillus thermodenitrificans DSM-465 characterization and in silico elucidation of conserved structural domains (2019), RSC Adv., 9, 4258-4267 .

No PubMed abstract available

Search for more literature for 3.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus thermodenitrificans

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular modeling of structure and docking with the substrate and potential inhibitors	Geobacillus thermodenitrificans

Inhibitors

Inhibitors	Comment	Organism	Structure
CB-839	i.e. N-[5-[4-[6-[[2-[3-(trifluoromethoxy)phenyl]acetyl]amino]-3-pyridazinyl]butyl]-1,3,4-thiadiazol-2-yl]-2-pyridineacetamide, is the best inhibitor as designated by the binding free energy changes	Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.104	-	L-glutamine	pH 9.0, 70°C	Geobacillus thermodenitrificans

Organism

Organism	UniProt	Comment	Textmining
Geobacillus thermodenitrificans	A4IPX2	-	-
Geobacillus thermodenitrificans NG80-2	A4IPX2	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + H2O	-	Geobacillus thermodenitrificans	L-glutamate + NH3	-	?
L-glutamine + H2O	-	Geobacillus thermodenitrificans NG80-2	L-glutamate + NH3	-	?

Subunits

Subunits	Comment	Organism
?	x * 33000, SDS-PAGE	Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	-	Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Geobacillus thermodenitrificans

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Release 2023.1 (January 2023)